[摘要] 目的：探讨抗氧化剂N-乙酰半胱氨酸(NAC)和川芎嗪对博莱霉素所致小鼠肺间质纤维化的治疗作用及机制。方法：博莱霉素(BLM)制成小鼠肺间质纤维化模型。各组动物处死后提取肺组织和支气管肺泡灌洗液(BALF),进行病理半定量分析,检测谷胱甘肽(GSH)和丙二醛(MDA)含量、BALF中转化生长因子-β(TGF-β)水平。结果： NAC和川芎嗪治疗组肺泡炎、纤维化病变均较模型组减轻，7 d时肺组织GSH量较模型组高(P<0.05), MDA含量较模型组低(P<0.05)，7、14 d时TGF-β蛋白水平较模型组低(P<0.05)。结论：NAC和川芎嗪可提高肺组织GSH含量，降低MDA生成，使肺泡炎和肺纤维化病变减轻。NAC和川芎嗪通过抗氧化作用，减少TGF-β产生，是其治疗肺纤维化的机制之一。

    [关键词] 肺纤维化;博莱霉素;N-乙酰半胱氨酸;川芎嗪;TGF-β;小鼠

    The Effect of N-acetylcysteine and Tetramethylpyrazine on Bleomycin-induced Pulmonary Fibrosis and TGF-β

    ZHANG Jin-hong, TU Ming-li, LEI Huai-din, LIU Wei-shun

    (Department of Respiratory diseases, Taihe Hospital, Yunyang Medical College, Shiyan, Hubei 442000, China)

    Abstact: Objective To investigate the therapeutic effect of N-acetylcysteine and tetramethylpyrazine in bleomycin-induced pulmonary fibrosis in mice and explore its mechanism.Methods Fifty male Kunming mice were randomly categorized into four groups:the bleomycin(BLM) group,the N-acetylcysteine(NAC) treated group, the Tetramethylpyrazine treated group and the control group.Pulmonary fibrosis model was induced by intratracheal instillation of single dose BLM 5 mg·kg-1 in mice. NAC and Tetramethylpyrazine treated group were given with NAC 80 mg·kg-1 and Tetramethylpyrazine 40 mg·kg-1 ......