[摘要] 目的:探讨藤梨根正丁醇提取物对培养的人食管癌Eca-109细胞的生长抑制作用。方法:MTT比色法检测藤梨根正丁醇提取物在不同浓度(1 μg/ml、10 μg/ml、100 μg/ml)及不同时间(24 h、48 h、72 h)对Eca-109细胞生长抑制作用， TUNEL法检测其对癌细胞生长的诱导凋亡效应。结果:①藤梨根正丁醇提取物对人食管癌Eca-109细胞的生长抑制作用随着药物浓度的升高和作用时间的延长而增强。②提取物对瘤细胞有明显的凋亡效应。结论:藤梨根正丁醇提取物能有效抑制人食管癌Eca-109细胞生长。

    [关键词] 藤梨根/猕猴桃根；食管肿瘤；抑制；凋亡

    Inhibitory Effect of Extracts From Actinidia Arguta by N-butyl Alcohol on Human Carcinoma of Esophagus Cells

    ZHANG Li, GUO Hong-li, TIAN Lin, CAO Shu-fen， DU Chang-hai.

    (Department of Pathology, Renmin Hospital， Yunyang Medical College, Shiyan, Hubei 442000； Department of Pathology， Yunyang Medical College, Shiyan, Hubei 442000)

    Abstract:Objective To evaluate the inhibitory effect of extracts from Actinidia arguta by n-butyl alcohol on human carcinoma of esophagus cells(Eca-109).Methods MTT colormetric assay was used to examine the growth inhibition of extracts from Actinidia arguta by n-butyl alcohol on Eca-109 cells, TUNEL test was performed to observe the apoptosis induced by the extracts (1 μg/ml、10 μg/ml、100 μg/ml)on Eca-109 cells. Results ① The inhibitory effect of the extracts on Eca-109 cells was increased in a dose-and time-dependent manner. ②The extracts could significantly induce apoptosis of Eca-109 cells. Conclusion The extracts from Actinidia arguta by n-butyl alcohol could effectively inhibit Eca-109 cell growth. ......