谷胱甘肽转硫酶M1基因型与胃癌易感性的研究
胃肿瘤·基因型·流行病学,分子,,胃肿瘤·基因型·流行病学，分子,1材料与方法,2结果,3讨论,参考文献
【摘要】目的：探讨中国汉族人群GSTM1基因型与胃癌易患性的关系，为胃癌高危人群的确定和胃癌的一级预防提供分子生物学手段。方法：501例，分为胃癌(GC)组、慢性萎缩性胃炎(CAG)组、慢性浅表性胃炎(CSG)组、胃溃疡(GU)组、十二指肠溃疡(DU)组及正常对照组，采用统一的调查表，调查年龄、性别、学历、职业、吸烟史、饮酒史、饮食习惯、肿瘤家族史等。采用序列特异性引物的聚合酶链反应(PCRSSP)检测GSTM1基因型测定组的基因型频率，ELISA法测定Hp感染，病例对照研究方法进行流行病学分析。结果：单因素分析显示与胃癌有关联的危险因素为年龄、性别、文化程度、职业、HP感染、吸烟、GSTM1空白基因型，其中大蒜为保护性因素；多因素分析显示与胃癌有关联的危险因素分别为HP感染、GSTM1空白基因型，大蒜仍为保护性因素。HP感染与GSTM1空白基因型对胃癌的发生存在协同作用；吸烟与GSTM1空白基因型存在协同作用。结论：具有GSTM1基因型的个体发生胃癌的危险性增加，该基因型可以认为是胃癌易患性的标志。检测该基因型能够对胃癌高危人群的确定，对胃癌的早期发现和一级预防提供有益的线索。【关键词】胃肿瘤·基因型·流行病学，分子
The study of the association of GSTM1 gene polymorphism with susceptibility to gastric cancer
ZHOU Tao1,FAN Wei1, HAN Wei1,GAO Yanjing1,YUAN Mengbiao1,LI Junman1,LI Huiqing2,LI Yanqing1
1Department of Gastroenterology,Qilu Hospital of Shandong University(Jinan 250012,China)
2Institute of Basic Medicine,Shandong Academy of Medical Sciences(Jinan 250062,China)
【ABSTRACT】Objective:To explore the association of GSTM1 gene polymorphism with susceptibility to gastric cancer in Chinese Han nationality in order to provide molecular measure to definethe high risk population of gastric cancer and for primary prevention.Methods:All subjects have been divided into Gastric cancer(GC),chronic atrophic gastritis(CAG),chronic superficial gastritis(CSG),gastric ulcer,duodenal ulcer and control groups which had been diagnosed by means of endoscopical and histopathological observation.Data on lifestyle have been routinely collected by means of common questionnaire sheets.The questionnaire comprises questions on age,sex,education ,occupation,smoking and drinking,dietary habits and family history of cancer,et al.GSTM1 genotypes were detected by polymerase chain reaction of sequencespecific primers(PCRSSP).Hp infection was detected by enzymelinked immunosorbent assay (ELISA).The approach of case control was used by the analyses of risk fators.Results:The risk factors associated with the development of gastric cancer were age,sex ,education,occupation,helicobacter pylori infection,tobacco smoke,GSTM1 null genotype,garlic was protective factor.Multivariate analysis have shown that the risk factors were helicobacter pylori infection,GSTM1 null genotype,garlic was also a protective factor.There is synergistic action between helicobacter pylori infection and GSTM1 genotype,tobacco smoke and GSTM1 genotype in the development of gastric cancer.Conclusion:The GSTM1 null genotype,helicobacter pylori infection and tobacco smoke was associated with susceptibility to gastric cancer.Individuals with this genotype has risk associated with the development of gastric cancer,this genotype may be considered as a marker associated with susceptibility to gastric cancer,and detecting this susceptible genotype can provide the clue to define the high risk population of gastric cancer and for diagnosis in early stage and for primary prevention. ......
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