健脾疏肝方对反流性食管炎大鼠模型食管组织病理及PCNA的影响
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李春婷, 沈水杰, 杜 斌, 滑永志, 蒋凤荣, 印 勇
健脾疏肝方;反流性食管炎;增殖细胞核抗原,李春婷,沈水杰,杜斌,滑永志,蒋凤荣,印勇,通讯作者:,Effectofliver-soothingandspleen-strengtheningdecoctiononesophagealpa
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李春婷, 沈水杰, 杜斌, 滑永志, 蒋凤荣, 印勇, 南京中医药大学中医内科教研室 江苏省南京市 210029
江苏省高校自然科学研究计划项目, No. 03KJB360100
通讯作者: 李春婷, 210029, 江苏省南京市汉中路282号61信箱, 南京中医药大学中医内科教研室. xt010503@sina.com
电话: 025-85811644
收稿日期: 2006-12-11 接受日期: 2007-01-05
Effect of liver-soothing and spleen-strengthening decoction on esophageal pathology and proliferating cell nuclear antigen expression in rats with reflux esophagitis
Chun-Ting Li, Shui-Jie Shen, Bin Du, Yong-Zhi Hua, Feng-Rong Jiang, Yong Yin
Chun-Ting Li, Shui-Jie Shen, Bin Du, Yong-Zhi Hua, Feng-Rong Jiang, Yong Yin,Department of Traditional Chinese Internal Medicine, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China
Supported bythe Natural Science Research Foundation of Jiangsu Provincial Higher Education, No. 03KJB360100
Correspondence to: Chun-Ting Li, Department of Traditional Chinese Internal Medicine, 282 Hanzhong Road, Nanjing 210029, Jiangsu Province, China. xt010503@sina.com
Received:2006-12-11 Accepted:2007-01-05
Abstract
AIM: To investigate the effect of liver-soothing and spleen-strengthening (LSSS) decoction on esophageal pathology and proliferating cell nuclear antigen (PCNA) expression in rats with reflux esophagitis.
METHODS: Partial pylori ligation plus cardiomyotomy was used to establish the rat models of reflux esophagitis. A total of 240 rats were divided into 7 groups, including normal group, model group, placebo group, positive control group, and Chinese medicine groups (high-, moderate-, and low-dose group). After treatment with the corresponding methods, HE staining was performed to observe the pathological changes of esophageal tissues, while the expression of PCNA was measured by immunohistochemistry.
RESULTS: LSSS decoction remarkably alleviated the esophageal pathological changes, the pathological score was significantly different between high-dose LSSS decoction group and model group. As compared with that in model group, the expression of PCNA was significantly decreased in LSSS decoction groups (low dose: 284.83 ± 30.49 vs 330.00 ± 35.35, P < 0.05; moderate dose: 239.67 ± 28.84 vs 330.00 ± 35.35, P < 0.01; high dose: 203.00 ± 25.29 vs 330.00 ± 35.35, P < 0.01). There was no marked difference between LSSS decoction groups and positive control group.
CONCLUSION: LSSS decoction may restrain the high expression of PCNA while alleviate the symptoms and esophageal inflammation of reflux esophagitis.
Key Words: Liver-soothing and spleen-strengthening decoction; Reflux esophagitis; Proliferating cell nuclear antigen
Li CT, Shen SJ, Du B, Hua YZ, Jiang FR, Yin Y. Effect of liver-soothing and spleen-strengthening decoction on esophageal pathology and proliferating cell nuclear antigen expression in rats with reflux esophagitis. Shijie Huaren Xiaohua Zazhi 2007;15(7):733-736
摘要 ......
李春婷, 沈水杰, 杜斌, 滑永志, 蒋凤荣, 印勇, 南京中医药大学中医内科教研室 江苏省南京市 210029
江苏省高校自然科学研究计划项目, No. 03KJB360100
通讯作者: 李春婷, 210029, 江苏省南京市汉中路282号61信箱, 南京中医药大学中医内科教研室. xt010503@sina.com
电话: 025-85811644
收稿日期: 2006-12-11 接受日期: 2007-01-05
Effect of liver-soothing and spleen-strengthening decoction on esophageal pathology and proliferating cell nuclear antigen expression in rats with reflux esophagitis
Chun-Ting Li, Shui-Jie Shen, Bin Du, Yong-Zhi Hua, Feng-Rong Jiang, Yong Yin
Chun-Ting Li, Shui-Jie Shen, Bin Du, Yong-Zhi Hua, Feng-Rong Jiang, Yong Yin,Department of Traditional Chinese Internal Medicine, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China
Supported bythe Natural Science Research Foundation of Jiangsu Provincial Higher Education, No. 03KJB360100
Correspondence to: Chun-Ting Li, Department of Traditional Chinese Internal Medicine, 282 Hanzhong Road, Nanjing 210029, Jiangsu Province, China. xt010503@sina.com
Received:2006-12-11 Accepted:2007-01-05
Abstract
AIM: To investigate the effect of liver-soothing and spleen-strengthening (LSSS) decoction on esophageal pathology and proliferating cell nuclear antigen (PCNA) expression in rats with reflux esophagitis.
METHODS: Partial pylori ligation plus cardiomyotomy was used to establish the rat models of reflux esophagitis. A total of 240 rats were divided into 7 groups, including normal group, model group, placebo group, positive control group, and Chinese medicine groups (high-, moderate-, and low-dose group). After treatment with the corresponding methods, HE staining was performed to observe the pathological changes of esophageal tissues, while the expression of PCNA was measured by immunohistochemistry.
RESULTS: LSSS decoction remarkably alleviated the esophageal pathological changes, the pathological score was significantly different between high-dose LSSS decoction group and model group. As compared with that in model group, the expression of PCNA was significantly decreased in LSSS decoction groups (low dose: 284.83 ± 30.49 vs 330.00 ± 35.35, P < 0.05; moderate dose: 239.67 ± 28.84 vs 330.00 ± 35.35, P < 0.01; high dose: 203.00 ± 25.29 vs 330.00 ± 35.35, P < 0.01). There was no marked difference between LSSS decoction groups and positive control group.
CONCLUSION: LSSS decoction may restrain the high expression of PCNA while alleviate the symptoms and esophageal inflammation of reflux esophagitis.
Key Words: Liver-soothing and spleen-strengthening decoction; Reflux esophagitis; Proliferating cell nuclear antigen
Li CT, Shen SJ, Du B, Hua YZ, Jiang FR, Yin Y. Effect of liver-soothing and spleen-strengthening decoction on esophageal pathology and proliferating cell nuclear antigen expression in rats with reflux esophagitis. Shijie Huaren Xiaohua Zazhi 2007;15(7):733-736
摘要 ......
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