大葱提取液抗人胃癌作用的细胞周期特点
大葱；胃肿瘤；分化；凋亡；细胞周期,,大葱；胃肿瘤；分化；凋亡；细胞周期,1材料与方法,2结果,3讨论,参考文献
【摘要】目的观察大葱提取液抗胃癌作用的细胞周期特点。方法将大葱提取液作用于体外培养的人胃癌细胞MGC803后，采用电镜观察MGC803细胞超微结构变化;应用流式细胞仪分析MGC803细胞DNA含量及周期分布，检测分化与凋亡。结果(1)透射电镜下观察大葱作用后的癌细胞,细胞膜完整，出现分化和凋亡的形态学变化。(2)流式细胞仪检测显示:1.25%浓度大葱提取液作用24h、48h、72h、96h ,凋亡率分别为0.6%、1.6%、6.1%、13.5%，二倍体比例分别为0%、0.3%、35.8%、93.9%;1.25%、2.5%、5.0%、10.0%浓度大葱提取液作用24 h，凋亡率分别为0.6%、4.1%、6.2%、24.9%，二倍体比例分别为0%、10.6%、34.7%、72.2%。大葱液低浓度组以诱导分化作用为主，高浓度组诱导分化同时，可诱导细胞凋亡。诱导凋亡作用呈剂量和时间依赖性。实验组细胞随大葱液浓度和作用时间增加，二倍体细胞比例逐渐增多，异倍体细胞比例逐渐减少，细胞凋亡率逐渐增加。DNA含量出现二倍体峰和亚二倍体峰～凋亡峰。大葱提取液抑制胃癌细胞生长增殖、诱导细胞凋亡可发生在G0/G1期、S期和G2/M期，无周期特异性。结论大葱提取液能明显抑制体外培养的胃癌细胞生长增殖，既可诱导胃癌细胞分化，又可诱导胃癌细胞凋亡，抗癌作用无周期特异性。【关键词】大葱；胃肿瘤；分化；凋亡；细胞周期
Cell cyclical characteristic of anti-human gastric cancer cell line MGC803 of extracts of Allium Fistulosum.L (AF)TAN Hong,CHANG Lili,GAO Fugui Health Supervision of Admistration in Hebei Province, Shijiazhuang050071,China
【Abstract】ObjectiveTo study the Cell cyclical characteristic of antigastric cancer of extracts of AF.MethodsAfter treatment MGC803 with extracts of AF,the following parameters are examined：observing the ultrastructure of MGC803 with electron microscope；cell DNA contents,cell cycle distribution,apoptosis and differentiation of MGC803 are tested by flow cytometry.Results(1) With transmission electron electron microscope,MGC803 cell membrane keeps completed,cell ultrastructure changes as typical apoptosis and differentiation.(2) The results of flow cytometry indicate:① After being treated with 1.25% concentration of AF,apoptotic rates of MGC803 are 0.61%,1.6 1%,6.08%,13.54% respectively ......
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