宫颈肿瘤；癌基因；HCCR2基因_

人子宫颈癌基因HCCR2真核表达载体的构建及鉴定

http://www.100md.com 《第四军医大学学报》 2007年第3期

宫颈肿瘤；癌基因；HCCR2基因,,宫颈肿瘤；癌基因；HCCR2基因,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Construction and identification of recombinant eukaryotic expression vector of human cervical cancer oncogene HCCR2

    GUO Jun, FANG DianChun，JIANG Feng，YANG ShiMing

    Research Center of Gastroenterology， Southwest Hospital, Third Military Medical University, Chongqing 400038, China,Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xian 710038, China

    【Abstract】 AIM： To clone and construct the recombinant eukarotic plasmid of human cervical cancer oncogene HCCR2 and provide the premise for its application to study the mutual relation between HCCR2 and P53 in gastric cancer. METHODS: Total RNA was isolated from hepatocellular carcinoma cell line HepG2. Subsequently, a 1kb fragment of HCCR2 encoding region was amplified by RTPCR and cloned into Promega TEasy vector. After confirmed by DNA sequencing, the fulllength encoding region of HCCR2 was amplified by PCR and cloned into pIRES2EGFP. The recombinant eukaryotic expression vector was confimed by DNA sequencing. RESULTS: A 1 kb fragment and a 0.9 kb fragment of HCCR2 was successfully amplified by RTPCR and PCR respectively. DNA sequencing suggested that both HCCR2/T and pIRES2EGFP/HCCR2(+) possesses correct read frame and nucleotide sequence of HCCR2. CONCLUSION: The pIRES2EGFP/HCCR2(+) was successfully constructed and this laid a solid foundation for uncovering the carcinogenic mechanism of HCCR2. ......

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