Detecion of sIgE, tryptase, ILª²8, ILª²12, and eotaxin in plasma of patients with pollinosis in summer or autumn and their clinical implications

    YAO Qiª²Jian, HE Shaoª²Heng, LIN Xiaoª²Ping

    Institute of Allergy & Inflammation, Medical College, Shantou University, Shantou 515031, China, Room of Allergy, PLA Center for Diagnosis and Treatment of Respiratory and Allergic Diseases, General Hospital of Shenyang Military Area Command, Shenyang 110016, China

    ¡¾Abstract¡¿ AIM£ºTo investigate the correlations between plasma levels of tryptase, interleukinª²8 (ILª²8), interleukinª²12 (ILª²12) and eotaxin in the patients with grass pollinosis in summer and autumn and their clinical significances. METHODS£ºA total of 37 patients with grass pollinosis and 23 healthy controls were recruited into the study. Plasma tryptase levels were determined by Unicap Tryptase fluoroimmunoassay. The levels of ILª²8, ILª²12 and eotaxin were detected by double sandwich ELISA method. RESULTS£ºThe plasma level of ILª²8 was significantly increased in the patients with pollinosis as compared with healthy controls (t=2.56, P<0.05). In contrast, the plasma level of ILª²12 was significantly decreased in the patients with pollinosis as compared with the healthy controls (P<0.05). But the levels of tryptase and eotaxin had no significant differences between the patients and the volunteers (P>0.05). The significant correlations were only between ILª²8 and ILª²12 (P<0.01) and between ILª²8 and eotaª²xin (P<0.05). CONLUSION: Occurrence of pollinosis is related to many inflammatory cells including mast cells and inflammatory mediators. The downª²regulation of Th1 cytokines may contribute to the pathogenesis of pollinosis. ......