志贺菌基因转移耐多药相关蛋白初步分析
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宋春花, 黄志刚, 郗园林, 张梅喜, 段广才
志贺菌;耐多药;蛋白质组学;双向电泳;质谱分析;差异表达,宋春花,郗园林,张梅喜,段广才,黄志刚,宋春花,通讯作者:,电话:,收稿日期:,接受日期:,Primaryanalysisonconjugationaltransfermultidrug
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宋春花, 郗园林, 张梅喜, 段广才, 郑州大学公共卫生学院流行病学教研室 河南省郑州市 450001
黄志刚, 河北工程大学医学院 河北省邯郸市 056002
宋春花, 在读博士, 主要从事分子流行病学研究.
通讯作者: 段广才, 450001, 河南省郑州市科技大道100号, 郑州大学公共卫生学院流行病学教研室. gcduan@public.zz.ha.cn
电话: 0371-66969270
收稿日期: 2006-11-07 接受日期: 2007-01-20
Primary analysis on conjugational transfer multidrug resistance-related proteins of Shigella flexneri strain
Chun-Hua Song, Zhi-Gang Huang, Yuan-Lin Xi, Mei-Xi Zhang, Guang-Cai Duan
Chun-Hua Song, Yuan-Lin Xi, Mei-Xi Zhang, Guang-Cai Duan, Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, He’nan Province, China
Zhi-Gang Huang, Medical College of Hebei Engineering University, Handan 056002, Hebei Province, China
Correspondence to: Medical College of Hebei Engineering University, Handan 056002, Hebei Province, China
Received: 2006-11-07 Accepted: 2007-01-20
AbstractAIM:To search for the new proteins related to multidrug resistance by comparing the proteomics of whole cellular proteins between sensitive strain and conjugational transfer anti-multidrug strain of Shigella flexneri.
METHODS: Clinical sensitive Shigella flexneri strain was transduced into anti-multidrug strain by conjugational transfer trials. Immobilized pH gradient (IPG) two-dimensional electrophoresis was adopted and the gels were analyzed by Image Master 2D Platinum software. Matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) were used to analyzed differential expression proteins.
RESULTS: Conjugational transfer anti-multidrug strain of Shigella flexneri was obtained successfully. It was found that there were 946±37 protein spots in the whole cellular protein 2-DE gels of sensitive strain and 1013±157 protein spots in that of conjugational transfer anti-multidrug strain. A total of 43 differential expression protein spots were found and 5 proteins related to multidrug resistance, including two new proteins (CRISPR-associated protein and heat shock protein chaperone Groel-Groes-Adp7), were identified based on peptide mass fingerprinting. ATP binding cassette transporter protein, cysteine synthase, predicted periplasmic or secreted lipoprotein protein were highly expressed in conjugational transfer anti-multidrug strain.
CONCLUSION: Some genes related to multidrug resistance of the donor can be transduced into sensitive strains and expressed highly. Meanwhile, the expression of some important cellular metabolic enzymes is up-regulated, and ATP binding cassette transporter protein plays an significant role in the mechanism of Shigella flexneri multidrug resistance.
Key Words: Shigella flexneri; Multidrug resistance; Proteomics; Two-dimensional electrophoresis; Mass spectrum analysis; Differential expression
Song CH, Huang ZG, Xi YL, Zhang MX, Duan GC ......
宋春花, 郗园林, 张梅喜, 段广才, 郑州大学公共卫生学院流行病学教研室 河南省郑州市 450001
黄志刚, 河北工程大学医学院 河北省邯郸市 056002
宋春花, 在读博士, 主要从事分子流行病学研究.
通讯作者: 段广才, 450001, 河南省郑州市科技大道100号, 郑州大学公共卫生学院流行病学教研室. gcduan@public.zz.ha.cn
电话: 0371-66969270
收稿日期: 2006-11-07 接受日期: 2007-01-20
Primary analysis on conjugational transfer multidrug resistance-related proteins of Shigella flexneri strain
Chun-Hua Song, Zhi-Gang Huang, Yuan-Lin Xi, Mei-Xi Zhang, Guang-Cai Duan
Chun-Hua Song, Yuan-Lin Xi, Mei-Xi Zhang, Guang-Cai Duan, Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, He’nan Province, China
Zhi-Gang Huang, Medical College of Hebei Engineering University, Handan 056002, Hebei Province, China
Correspondence to: Medical College of Hebei Engineering University, Handan 056002, Hebei Province, China
Received: 2006-11-07 Accepted: 2007-01-20
AbstractAIM:To search for the new proteins related to multidrug resistance by comparing the proteomics of whole cellular proteins between sensitive strain and conjugational transfer anti-multidrug strain of Shigella flexneri.
METHODS: Clinical sensitive Shigella flexneri strain was transduced into anti-multidrug strain by conjugational transfer trials. Immobilized pH gradient (IPG) two-dimensional electrophoresis was adopted and the gels were analyzed by Image Master 2D Platinum software. Matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) were used to analyzed differential expression proteins.
RESULTS: Conjugational transfer anti-multidrug strain of Shigella flexneri was obtained successfully. It was found that there were 946±37 protein spots in the whole cellular protein 2-DE gels of sensitive strain and 1013±157 protein spots in that of conjugational transfer anti-multidrug strain. A total of 43 differential expression protein spots were found and 5 proteins related to multidrug resistance, including two new proteins (CRISPR-associated protein and heat shock protein chaperone Groel-Groes-Adp7), were identified based on peptide mass fingerprinting. ATP binding cassette transporter protein, cysteine synthase, predicted periplasmic or secreted lipoprotein protein were highly expressed in conjugational transfer anti-multidrug strain.
CONCLUSION: Some genes related to multidrug resistance of the donor can be transduced into sensitive strains and expressed highly. Meanwhile, the expression of some important cellular metabolic enzymes is up-regulated, and ATP binding cassette transporter protein plays an significant role in the mechanism of Shigella flexneri multidrug resistance.
Key Words: Shigella flexneri; Multidrug resistance; Proteomics; Two-dimensional electrophoresis; Mass spectrum analysis; Differential expression
Song CH, Huang ZG, Xi YL, Zhang MX, Duan GC ......
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