活血化瘀注射液I号预处理与缺血预处理改善肝缺血再灌注损伤
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毕旭东, 郭 伟, 赵 晶, 赵连根, 付晓光
肝脏缺血再灌注损伤;缺血预处理;肿瘤坏死因子-a;活血化瘀注射液I号;大鼠毕旭东,郭伟,赵晶,赵连根,付晓光.活血化瘀注射液I号预处理与缺血预处理改善肝缺血再灌注损伤. 世界华人消化杂志2007;15(8)831-837,毕旭东
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毕旭东, 付晓光, 辽宁医学院附属第一医院肝胆外科 辽宁省锦州市 121001
郭伟, 山东省肿瘤医院放射线科 山东省济南市 250117
赵晶, 辽宁医学院组织胚胎学教研室 辽宁省锦州市 121001
赵连根, 天津市中西医结合急腹症研究所 天津市 300100
毕旭东, 2000年天津医科大学博士, 副教授, 主要从事肝胆外科临床和基础研究.
通讯作者: 毕旭东, 121001, 辽宁省锦州市人民街5段2号, 辽宁医学院附属第一医院肝胆外科. bizhaojing@sohu.com
电话: 0416-4197248
收稿日期: 2006-09-28 接受日期: 2007-01-04
Effects of Huoxue Huayu injection I pretreatment and ischemic preconditioning on liver ischemia and reperfusion injury in rats
Xu-Dong Bi, Wei Guo, Jing Zhao, Lian-Gen Zhao, Xiao-Guang Fu
Xu-Dong Bi, Xiao-Guang Fu, Department of Hepatobiliary Surgery, the First Affiliated Hospital of Liaoning Medical College, Jinzhou 121001, Liaoning Province, China
Wei Guo, Department of Radiology, Shandong Tumor Hospital, Ji’nan 250117, Shandong Province, China
Jing Zhao, Department of Histoembryology, Liaoning Medical College, Jinzhou 121001, Liaoning Province, China
Lian-Gen Zhao, Tianjin Integrated Traditional Chinese and Western Medicine Institute of Acute Abdomen, Tianjin 300100, China
Correspondence to: Xu-Dong Bi, Department of Hepatobiliary Surgery, the First Affiliated Hospital of Liaoning Medical College, 2 Renmin Street 5 Section, Jinzhou 121001, Liaoning Province, China. bizhaojing@sohu.com
Received: 2006-09-28 Accepted: 2007-01-04
AbstractAIM:To investigate the effects of Huoxue Huayu injection I (HHI-I) pretreatment and ischemic preconditioning (IP) on hepatic ischemia and reperfusion (I/R) injury in rats and compare their efficacity.
METHODS: Eighty healthy adult male Sprague-Dawley rats were randomly and averagely divided into 4 groups: sham operation (SO) group, I/R group, ischemia preconditioning (IP) group, and HHI-I pretreatment group. After partial hepatic I/R model was made, the rats of each group were sacrificed to determine serum alanine aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), tissue malondialdehyde (MDA) and superoxide dismutase (SOD) level 1, 3, 6 and 24 hours after reperfusion, respectively. Tissue tumor necrosis factor alpha (TNF-a) and intercellular adhesion molecule-1 (ICAM-1) mRNA levels were determined by reverse transcription-polymerase chain reaction after 1 hour of reperfusion. Liver histology was observed after 3 hours of reperfusion.
RESULTS: The activities of ALT, AST, LDH, the level of MDA, and the expression of TNF-a, ICAM-1 mRNA in I/R, IP, and HHI-I group were significantly higher than those in SO group, but they were markedly lower in IP and HHI-I group than those in I/R group. The activities of ALT, AST, LDH in HHI-I group were significantly lower than those in IP group (ALT: 2378.8 ± 303.4 nkat/L vs 2840.6 ± 248.4 nkat/L; AST: 2887.2 ± 270.1 nkat/L vs 4567.6 ± 275.1 nkat/L; LDH: 10550.4 ± 710.1 nkat/L vs 12164.1 ± 735.1 nkat/L; all P < 0 ......
毕旭东, 付晓光, 辽宁医学院附属第一医院肝胆外科 辽宁省锦州市 121001
郭伟, 山东省肿瘤医院放射线科 山东省济南市 250117
赵晶, 辽宁医学院组织胚胎学教研室 辽宁省锦州市 121001
赵连根, 天津市中西医结合急腹症研究所 天津市 300100
毕旭东, 2000年天津医科大学博士, 副教授, 主要从事肝胆外科临床和基础研究.
通讯作者: 毕旭东, 121001, 辽宁省锦州市人民街5段2号, 辽宁医学院附属第一医院肝胆外科. bizhaojing@sohu.com
电话: 0416-4197248
收稿日期: 2006-09-28 接受日期: 2007-01-04
Effects of Huoxue Huayu injection I pretreatment and ischemic preconditioning on liver ischemia and reperfusion injury in rats
Xu-Dong Bi, Wei Guo, Jing Zhao, Lian-Gen Zhao, Xiao-Guang Fu
Xu-Dong Bi, Xiao-Guang Fu, Department of Hepatobiliary Surgery, the First Affiliated Hospital of Liaoning Medical College, Jinzhou 121001, Liaoning Province, China
Wei Guo, Department of Radiology, Shandong Tumor Hospital, Ji’nan 250117, Shandong Province, China
Jing Zhao, Department of Histoembryology, Liaoning Medical College, Jinzhou 121001, Liaoning Province, China
Lian-Gen Zhao, Tianjin Integrated Traditional Chinese and Western Medicine Institute of Acute Abdomen, Tianjin 300100, China
Correspondence to: Xu-Dong Bi, Department of Hepatobiliary Surgery, the First Affiliated Hospital of Liaoning Medical College, 2 Renmin Street 5 Section, Jinzhou 121001, Liaoning Province, China. bizhaojing@sohu.com
Received: 2006-09-28 Accepted: 2007-01-04
AbstractAIM:To investigate the effects of Huoxue Huayu injection I (HHI-I) pretreatment and ischemic preconditioning (IP) on hepatic ischemia and reperfusion (I/R) injury in rats and compare their efficacity.
METHODS: Eighty healthy adult male Sprague-Dawley rats were randomly and averagely divided into 4 groups: sham operation (SO) group, I/R group, ischemia preconditioning (IP) group, and HHI-I pretreatment group. After partial hepatic I/R model was made, the rats of each group were sacrificed to determine serum alanine aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), tissue malondialdehyde (MDA) and superoxide dismutase (SOD) level 1, 3, 6 and 24 hours after reperfusion, respectively. Tissue tumor necrosis factor alpha (TNF-a) and intercellular adhesion molecule-1 (ICAM-1) mRNA levels were determined by reverse transcription-polymerase chain reaction after 1 hour of reperfusion. Liver histology was observed after 3 hours of reperfusion.
RESULTS: The activities of ALT, AST, LDH, the level of MDA, and the expression of TNF-a, ICAM-1 mRNA in I/R, IP, and HHI-I group were significantly higher than those in SO group, but they were markedly lower in IP and HHI-I group than those in I/R group. The activities of ALT, AST, LDH in HHI-I group were significantly lower than those in IP group (ALT: 2378.8 ± 303.4 nkat/L vs 2840.6 ± 248.4 nkat/L; AST: 2887.2 ± 270.1 nkat/L vs 4567.6 ± 275.1 nkat/L; LDH: 10550.4 ± 710.1 nkat/L vs 12164.1 ± 735.1 nkat/L; all P < 0 ......
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