脂质体介导核因子-κB诱捕物寡聚脱氧核苷酸对重症急性胰腺炎大鼠胰腺炎症因子mRNA表达和胰腺损伤的影响
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钟荣德, 周 杰, 廖柳清, 符方勇, 李湘竑, 林艺雄
脂质体;核转录因子;重症急性胰腺炎;诱捕物;胰腺损伤;电泳迁移率变动分析;逆转录-聚合酶链反应钟荣德,周杰,廖柳清,符方勇,李湘竑,林艺雄.脂质体介导核因子-kB诱捕物寡聚脱氧核苷酸对重症急性胰腺炎大鼠胰腺炎症因子mRNA表达和胰腺损伤的影响. 世界华人消化杂志2007;15(8)813
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钟荣德, 周杰, 符方勇, 李湘竑, 林艺雄, 广州市南方医科大学南方医院肝胆血管外科 广东省广州市 510515
廖柳清, 增城市妇幼保健院 广东省广州市 511300
钟荣德, 医学硕士, 主要从事肝胆、胰脾系统疾病的临床及基础研究.广东省自然科学基金项目, No. 32859
通讯作者: 周杰, 510515, 广东省广州市广州大道北1838号, 广州市南方医科大学南方医院肝胆血管外科.
jacky@fimmu.edu.cn
电话: 020-61641701
收稿日期: 2006-09-28 接受日期: 2007-01-04
Effect of liposome-mediated nuclear factor κB decoy oligodeoxynucleotide on the mRNA expression of inflammatory factors and injury of pancreas in rats with severe acute pancreatitis
Rong-De Zhong, Jie Zhou, Liu-Qing Liao, Fang-Yong Fu, Xiang-Hong Li, Yi-Xiong Lin
Rong-De Zhong, Jie Zhou, Fang-Yong Fu, Xiang-Hong Li, Yi-Xiong Lin, Department of Hapetobiliary and Vascular Surgery, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China
Liu-Qing Liao, Zengcheng Hospital of Maternal and Child Health Care, Guangzhou 511300, Guangdong Province, China
Supported by the Natural Science Foundation of Guangdong Province, No. 32859
Correspondence to: Dr. Jie Zhou, Department of Hapetobiliary and Vascular Surgery, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China. jacky@fimmu.edu.cn
Received: 2006-09-28 Accepted: 2007-01-04
AbstractAIM:To investigate the influences of liposome-mediated nuclear factor kB (NF-kB) decoy oligodeoxynucleotide (ODN) on the NF-κB activation, inflammatory factor mRNA expression and pancreatic injury in rats with severe acute pancreatitis (SAP).
METHODS: Except for those in sham operation group, the rest rats were injected with sodium taurocholate to establish the model of SAP, and then intravenously injected with naked ODN, liposome/decoy ODN complexes, liposome/scrambled ODN complexes, and normal saline, respectively, 1 hour later. Four hours after injection, the NF-kB activity was analyzed by electrophoretic mobility shift assay (EMSA), and the mRNA expression of intercellular adhesion molecule-1 (ICAM-1), interleukin-1a (IL-1a), IL-2, tumor necrosis factor-a (TNF-a) and vascular cell adhesion molecule-1 (VCAM-1) were assessed by reverse transcription-polymerase chain reaction (RT-PCR). Meanwhile, the serum amylase level, pancreatic wet/dry weight ratio and myeloperoxidase (MPO) content were detected.
RESULTS: The NF-kB activity and its related inflammatory factors were observably inhibited in liposome/decoy ODN group as compared with those in normal saline group, liposome/scrambled ODN group and naked ODN group (NF-kB activation: P < 0.05; ICAM-1: 0.75 ± 0.13 vs 1.39 ± 0.15, 1.37 ± 0.16, 1.32 ± 0.17, all P < 0.05; IL-1a: 0.64 ± 0.09 vs 1.34 ± 0.20, 1.30 ± 0.14, 1.25 ± 0.20, all P < 0.05; IL-2: 0.23 ± 0.08 vs 0.74 ± 0.13, 0.71 ± 0.12, 0.69 ± 0.14, all P < 0.05; TNF-a: 0.41 ± 0.13 vs 1.30 ± 0.17, 1.26 ± 0.17, 1.23 ± 0.20, all P < 0.05; VCAM-1: 0 ......
钟荣德, 周杰, 符方勇, 李湘竑, 林艺雄, 广州市南方医科大学南方医院肝胆血管外科 广东省广州市 510515
廖柳清, 增城市妇幼保健院 广东省广州市 511300
钟荣德, 医学硕士, 主要从事肝胆、胰脾系统疾病的临床及基础研究.广东省自然科学基金项目, No. 32859
通讯作者: 周杰, 510515, 广东省广州市广州大道北1838号, 广州市南方医科大学南方医院肝胆血管外科.
jacky@fimmu.edu.cn
电话: 020-61641701
收稿日期: 2006-09-28 接受日期: 2007-01-04
Effect of liposome-mediated nuclear factor κB decoy oligodeoxynucleotide on the mRNA expression of inflammatory factors and injury of pancreas in rats with severe acute pancreatitis
Rong-De Zhong, Jie Zhou, Liu-Qing Liao, Fang-Yong Fu, Xiang-Hong Li, Yi-Xiong Lin
Rong-De Zhong, Jie Zhou, Fang-Yong Fu, Xiang-Hong Li, Yi-Xiong Lin, Department of Hapetobiliary and Vascular Surgery, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China
Liu-Qing Liao, Zengcheng Hospital of Maternal and Child Health Care, Guangzhou 511300, Guangdong Province, China
Supported by the Natural Science Foundation of Guangdong Province, No. 32859
Correspondence to: Dr. Jie Zhou, Department of Hapetobiliary and Vascular Surgery, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China. jacky@fimmu.edu.cn
Received: 2006-09-28 Accepted: 2007-01-04
AbstractAIM:To investigate the influences of liposome-mediated nuclear factor kB (NF-kB) decoy oligodeoxynucleotide (ODN) on the NF-κB activation, inflammatory factor mRNA expression and pancreatic injury in rats with severe acute pancreatitis (SAP).
METHODS: Except for those in sham operation group, the rest rats were injected with sodium taurocholate to establish the model of SAP, and then intravenously injected with naked ODN, liposome/decoy ODN complexes, liposome/scrambled ODN complexes, and normal saline, respectively, 1 hour later. Four hours after injection, the NF-kB activity was analyzed by electrophoretic mobility shift assay (EMSA), and the mRNA expression of intercellular adhesion molecule-1 (ICAM-1), interleukin-1a (IL-1a), IL-2, tumor necrosis factor-a (TNF-a) and vascular cell adhesion molecule-1 (VCAM-1) were assessed by reverse transcription-polymerase chain reaction (RT-PCR). Meanwhile, the serum amylase level, pancreatic wet/dry weight ratio and myeloperoxidase (MPO) content were detected.
RESULTS: The NF-kB activity and its related inflammatory factors were observably inhibited in liposome/decoy ODN group as compared with those in normal saline group, liposome/scrambled ODN group and naked ODN group (NF-kB activation: P < 0.05; ICAM-1: 0.75 ± 0.13 vs 1.39 ± 0.15, 1.37 ± 0.16, 1.32 ± 0.17, all P < 0.05; IL-1a: 0.64 ± 0.09 vs 1.34 ± 0.20, 1.30 ± 0.14, 1.25 ± 0.20, all P < 0.05; IL-2: 0.23 ± 0.08 vs 0.74 ± 0.13, 0.71 ± 0.12, 0.69 ± 0.14, all P < 0.05; TNF-a: 0.41 ± 0.13 vs 1.30 ± 0.17, 1.26 ± 0.17, 1.23 ± 0.20, all P < 0.05; VCAM-1: 0 ......
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