RP-HPLC测定小儿腹泻外敷凝胶中丁香酚的含量 (pdf)

    【摘要】 目的 建立小儿腹泻外敷凝胶中丁香酚的含量测定方法。 方法 采用高效液相色谱法，色谱柱：Lichrospher C18柱，流动相：甲醇-水(65：35)，紫外检测波长：280nm，流速：1.0ml/min。 结果 丁香酚在0.108～1.724μg范围内线性关系良好(r=0.9999)；平均回收率为100.4%，RSD=1.84%。 结论 本方法简便、准确，可用于该制剂的质量控制。

    【关键词】 小儿腹泻外敷凝胶；高效液相色谱；丁香酚

    Determination of eugenol in Xiao’er Fuxie Waifu gel by RP-HPLC

    YUAN Hong-yu，Ou Ning，GE Qiao-xiang.The First Affiliated Hospital of Nanjing Medical University， Nanjing 210029,China

    【Abstract】 Objective To establish the determination of eugenol in Xiao’er Fuxie Waifu gel. Methods RP-HPLC method was developed with Lichrospher-C18 analysis column using methyl alcohol-water (65：35) as a mobile phase. The detection wavelength was 280 nm and flow rate was 1.0ml/min. Results The linear range of eugenol was from 0.108 to 1.724 μg (r= 0.9999). The average recovery was 100.4% with RSD= 1.84%. Conclusion The method is convenient and accurate. It can be used for the quality control of Xiao’er Fuxie Waifu gel.

    【Key words】 Xiao’er Fuxie Waifu gel； HPLC； eugenol

    小儿腹泻外敷凝胶是以丁桂散为基本方加味吴茱萸、胡椒等药材 ......