【摘要】 目的 观察非选择性一氧化氮合酶抑制剂L硝基精氨酸(LNA)对大鼠局灶性脑缺血诱导的神经元凋亡的影响,探讨LNA对脑缺血的保护作用及其机制。方法 健康雄性SD大鼠，体重250～300 g，随机分为假手术组(SH组)、缺血组(IS组)、LNA治疗组(LNA组)。IS和LNA组按缺血后时间分为三个亚组：2 h、6 h、12 h。每组6只大鼠。LNA组每次按20 mg/kg,ip给药，每日2次，连续3 d。IS组给等容量的生理盐水。插线法制备大鼠大脑中动脉阻断模型。采用流式细胞仪(FCM)检测脑组织细胞凋亡率，Western blot法和免疫组化法检测Caspase3蛋白的表达，FCM和免疫组化法检测Bcl2和Bax蛋白的表达。结果 大鼠脑缺血后，细胞凋亡率、Caspase3和Bax蛋白表达明显升高，Bcl2蛋白表达差异无显著性，Bcl2/Bax降低，缺血12 h治疗3 d时,LNA组中细胞凋亡率、Caspase3和Bax蛋白表达显著低于相应的IS组，Bcl2蛋白表达、Bcl2 /Bax高于IS组，缺血2、6 h治疗3 d LNA组中细胞凋亡率、Caspase3、Bcl2、Bax蛋白表达和Bcl2/Bax与IS组比，差异无显著性。结论 缺血后期应用LNA对脑缺血有治疗作用，可能与降低Caspase3、增加Bcl2表达、降低Bax蛋白表达、调节Bcl2/Bax蛋白平衡有关。

    【关键词】 脑缺血；L硝基精氨酸；细胞凋亡

    Effect of NGnitroLarginine on neuronal apoptosis induced by focal cerebral ischemia in rats

    ZHANG Jianxin,ZHANG Huixin,LI Lanfang,et al.

    Hebei Academy of Medical Sciences,Shijiazhuang 050021

    【Abstract】 Objective By evaluating the effect of NGnitroLarginine (LNA) on neuronal apoptosis induced by transient focal cerebral ischemia in rats, to investigate the effect of LNA on cerebral ischemic injury and the possible mechanism.Methods Male SD rats (weighing 250～290 g) were randomly divided into 3 groups:sham operation group (SH),ischemic group (IS) and LNA group (LNA).IS and LNA group were further divided into 3 subgroups (n=6 each) according to the duration of ischemia 2 h,6 or 12 h.In LNA group,LNA (20 mg/kg,ip) was administrated,twice a day,for 3 consecutive days.Saline was administrated in IS group.The focal cerebral ischemic model (MCAO) with thread embolism of left middle cerebral artery was made.In sham operation group,the external carotid artery was surgically prepared for insertion of the filament,but the filament was not inserted.Rats were killed at the scheduled time.Apoptosis was detected by FCM.The expression of Caspase3 protein was observed by Western blot and immunohistochemistry.The expression of Bcl2 and Bax protein was measured by immunohistochemistry and FCM.Results After MCAO,the apoptotic rate of cells,the expression of Caspase3 and Bax protein was increased significantly.The ratio of Bcl2/Bax was decreased.The difference of Bcl2 protein was not significant.In LNA 12 h group,the percentage of apoptotic cells,the expression of Caspase3 and Bax protein was decreased.The expression of Bcl2 and the ratio of Bcl2/Bax was increased.There was no significant difference in the percentage of apoptotic cells,the expression of Caspase3,Bax,Bcl2 protein and Bcl2/Bax between LNA 2,6 h group and IS group.Conclusion LNA has beneficial effect on ischemic rat brain at ischemic later stage.The possible protective mechanism is that administration of LNA could reduce the expression of Caspase3 and Bax,protein,increase the expression of Bcl2 protein,and regulate the balance between Bcl2 and Bax protein. ...... 婵犵鈧啿鈧綊鎮樻径鎰畺闁靛ǹ鍎查煬顒€霉閿濆牊纭堕柡浣靛€濋獮宥団偓锝庡亝缁ㄦ氨绱掑☉姗嗘Ч缂佷緤濡囬幉鎾醇閻斿壊妲梺鍝勫暟婵數娑甸崼鏇炶摕闁靛／鍕暯闂佺懓鐡ㄩ悧鏃傜箔閸涱喗濮滈柦妯侯槸瀵潡鏌￠崒姘カ缂佽鲸绻堝畷锝夘敍濠靛棗骞嬮梺鍝勫閸ㄧ敻锝為敃鍌氱婵犲﹤鎳庨崑宥団偓娈垮枟濞叉﹢骞栭崨濠傜窞閺夊牃鏅滈崺鍌炴煃閵夛妇鐭庢繛鏉戞閹秵鎷呭ù瀣壕婵犲灚鎸剧粈澶愭偣閸ヮ剦妫戦柛瀣剁秮瀹曟瑧鎷犻懠顒佺秳濡ょ姷鍋炲ú妯恒€掗懜鐢碘枖閻庯綆鍋呴悡娆撴煟閵娿儱顏ら柍褜鍓氱划搴ㄥ极閹间焦鍤囬柟瀵稿Т椤ｅジ鏌嶉妷锔剧畺闁搞劊鍔戦崹鎯р堪閸愩劍鏋剧紓鍌氬暞閸ㄥ潡濡存径鎰伋婵犻潧饪撮崯蹇涙⒒閸屻倕鏋旈柍褜鍓ㄩ幏锟�


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