HL60细胞；PBK_TOPK；细胞分化；佛波醇酯类

PBK/TOPK在TPA诱导的人HL60细胞分化中的表达及意义

http://www.100md.com 《第四军医大学学报》 2007年第4期

HL60细胞；PBK,TOPK；细胞分化；佛波醇酯类,,HL60细胞；PBK,TOPK；细胞分化；佛波醇酯类,【关键词】HL60细胞；PBK,TOPK；细胞分化；佛波醇酯类,0引言,1材料和方法,2结果,3讨论,【参考文献

     Expression and significance of PBK/TOPK in differentiation of human HL60 leukemic cells induced by TPA

    LIU YuHong1, LIU YanXiang1, HUANG GaoSheng2, WANG WenQing2, ZHANG WeiPing2, WANG JuanHong2, LI YuanFei2, WANG Lu2, HE FuRong2, WANG Di2, WANG Zhe2

    1Department of Hematology, Affiliated Hospital, Yanan University, Yanan 716000, China, 2Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To investigate the expression and significance of PBK/TOPK in the differentiation of human HL60 leukemic cells induced by TPA. METHODS: After induction of TPA, WrightGiemsa staining was performed to observe the morphological changes of HL60 cells and flow cytometry was used to observe the cell cycles and the expressions of CD11b, CD14, CD13, CD33, respectively. Expression of PBK/TOPK was detected by Western Blot. RESULTS: After the treatment with 5.1 nmol/L TPA for 3 d, the number of NBT positive cells and the expressions of CD11b, CD13 and CD14 increased, and the expression of PBK/TOPK decreased. CONCLUSION: TPA can inhibit the proliferation of HL60 cells and induce the differentiation of HL60 cells into the granulocytic or monocytic lineage. During the process, PBK/TOPK expression is downregulated and the expression of PhoPBK/TOPK is upregulated. ......

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