Immunoactivity analysis of album pollen allergen

    ZHANG Jie, SUN Xiuª²Zhen, CHEN Xi, FENG Xiangª²Li, LIU Yanª²Qin

    Department of Pulmonary Medicine, Second Hospital, Medical College, Xi¬ðan Jiaotong University Xi¬ðan 710004, China

    ¡¾Abstract¡¿ AIM: To analyse protein components in album pollen and evaluate their allergenicity and immunogenicity. METHODS: The components of protein allergen were collected from the crude extract of chenopodium album pollen with Sephacryl Sª²200 HR gel filtration for removing the impurities. Molecular weights of the components were tested through SDSª²PAGE. Then the reaction rates of IgE and IgG in asthmatic patients serum to chenopodium album pollen were counted by immunoblotting. RESULTS: Twelve bands of protein were obtained through SDSª²PAGE. The molecular weight of each band was 92, 63.1, 61, 52, 45, 43, 39.7, 38.9, 34, 31.6, 28.4 and 18.5~12 ku. Immunobloting identified 4 protein bands recognized by sIgE, molecular weights being 92, 34, 31.6 and 18.5~12 ku, which reacted with 70%, 50%, 80% and 90% of asthmatic patient serum. Eight protein bands with molecular weights being 92, 61, 52, 45, 43, 39.7, 31.6 and 18.5~12 ku showed sIgG binding capacity, which reacted with 80%, 40%, 10%, 20%, 80%, 10%, 10% and 100% of asthmatic patient serum. CONCLUSION: Album pollen comprises 12 major protein components. Components with strong allergenicity and immunogenicity are the proteins with molecular weight of 18.5~12 and 92 ku. Proteins with molecular weight of 34 and 31.6 ku have strong allergenicity, and weak immunogenicity or not. Proteins with molecular weight of 61,43,45,52 and 39.7 ku possess certain immunogenicity, but no allergenicity. ......