胆管肿瘤；转化生长因子β1；免疫组织化学_

转化生长因子β1在胆管癌组织中的表达及意义

http://www.100md.com 《滨州医学院学报》 2006年第6期

胆管肿瘤；转化生长因子β1；免疫组织化学,,胆管肿瘤；转化生长因子β1；免疫组织化学,1材料和方法,2结果,3讨论,参考文献

     【摘要】目的研究转化生长因子β1(TGFβ1)在胆管癌组织中的表达，探讨它与胆管癌生物学行为及预后的关系。方法应用免疫组织化学SP法检测47例胆管癌及癌旁正常胆管组织中TGFβ1的表达，分析其表达与胆管癌临床病理特征的关系，结果进行统计学处理。结果 TGFβ1阳性表达主要定位于胆管癌细胞胞浆，偶有胞膜、胞核着色。47例胆管癌组织中TGFβ1阳性表达36例(766%)，较癌旁正常胆管组织高(P<0001)。TGFβ1表达与胆管癌临床分期及是否有淋巴结、肝转移有关(P<001，005，005)，与组织学分级无关(P>005)。结论 TGFβ1可能参与了胆管癌的发生、发展。随着胆管癌临床分期的增高而TGFβ1表达增高，其基因缺失可能是胆管癌细胞侵袭性生长转移的重要分子机制，检测TGFβ1表达有助于判断胆管癌的恶性程度及预后。

    【关键词】胆管肿瘤；转化生长因子β1；免疫组织化学

    Expression of TGFβ1 in cholangiocarcinoma tissues and its biological significance

    ZHANG Jianyu HU Zongling ZHANG Bingyuan et al

    People's Hospital of Ju County，276500

    【Abstract】 Objective To study the expression of TGFβ1 in cholangiocarcinoma tissues and its biological behavior.Methods TGFβ1 was detected by immunohistochemical methods in 47 paraffinembedded specimens of cholangiocarcinoma and the control tissues near to the tumor. Results Expression of TGFβ1 was significantly higher in cholangiocarcinoma than that of control tissues near to the tumor(P<0001).There was significantly relation between TGFβ1 expression and clinical stages,lymph node and liver metastasis(P<001,0.05,0.05),and no difference in histological grade. Conclusion Smad4 and TGFβ1,TGFβRⅡ may play an important role in the occurrence and development of cholangiocarcinoma. Loss of Smad4 and TGFβRⅡgene may be one of the significant molecular biological mechanism. ......

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