乙型肝炎；HBVDNA；前S1抗原____

乙肝小三阳血清HBVDNA定量检测及其与前S1关系的研究

http://www.100md.com 《实用医技杂志》 2007年第6期

乙型肝炎；HBVDNA；前S1抗原,,],乙型肝炎；HBVDNA；前S1抗原,1材料与方法,2结果,3讨论,参考文献：

     [摘要] 目的：检测乙型肝炎小三阳患者HBVDNA含量，并与前S1抗原(PreS1)进行比较分析。方法：收集351例乙型肝炎小三阳患者血清和30例正常体检者血清，用ELISA方法检测前S1抗原，用荧光定量PCR方法定量检测HBVDNA。结果：HBsAg、抗HBe、抗HBc阳性(小三阳)患者HBVDNA检出率为62.7%(4.5×106 copies/ml)，前S1抗原检出率为72.9%。结论:HBeAg转阴者也存在病毒复制，HBVDNA与前S1抗原相关性较好，对于乙型肝炎患者在常规血清学标志物检测的基础上对HBVDNA数量进行动态检测有助于临床诊断、疗效观察及预后判断。

    [关键词] 乙型肝炎；HBVDNA；前S1抗原

    Study the Relationship of HBVDNA Quantitatively Detection and PreS1 for the Patients of Hepatitis B with Positive of HBsAg、AntiHbe and AntiHBc

    LI Qiang,ZHANG Jianyuan,PANG Xueyun,LIU Ruiping

    (The Second Clinical Medical College of Guangzhou University of Traditional Chinese Medicine,Guangzhou，Guangdong 510120,China)

    Abstract：Objective To determine hepatitis B virusDNA(HBVDNA) and pre S1 antigen in patients with hepatitis B and expolore their different clinical values.Methods 351 positive serum of HBsAg,antiHBe,antiHBc of hepatitis B and 30 healthy man wrer collected.PreS1 antigen and HBV markets were detected with ELISA and HBVDNA with fluorescent quantitative PCR(FQPCR) respectively.Results The positive rates of HBVDNA and PreS1 antigen were 62.7%(4.5×106 copies/ml)、72.9% respective in patients with hepatitis B of which HBsAg、antiHBe、antiHBc were positive.Conclusion There were replication of virus in patients with HBeAg negative,The serum levels of HBVDNA of patients with hepatitis B closely correlate PreS1.HBVDNA with fluorescent quantitative PCR for the patients with hepatitis B is helpful to clinical diagnosis、clinical evalution and prognosis. ......

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