Study on therapy of experimental autoimmune neuritis by bone marrow stromal cells

    XU Xin, SUN Bo, MU Liª²li, WANG Guangª²you, JIN Lianª²hong, LI Huª²lun*

    Department of Neurobiology, Harbin Medical University, Key Laboratory of Heilongjiang Province, Harbin 150086, China

    \[Abstract\] AIM: To investigate the therapeutic effect of bone marrow stromal cells (BMSCs) transplantation on experimental autoimmune neuritis (EAN) and study the possible mechanism. METHODS: EAN model was established by immunizing Lewis rats with P0180ª²199 peptide and complete Freund¡¯s adjuvant (CFA). In the therapy group, BMSCs (2¡Á106 cells/rat) were marked with fluorochrome PKH26 and injected into the rats¡¯ caudal vein 10 d after immunization. The therapeutic effect of BMSC transplantation on EAN rats was investigated by clinical assessment, immunohistochemical staining, and ELISA, respectively. RESULTS: The injected BMSCs could migrate to the demyelinated nerve tissues, and the demyelination and inflammatory infiltration was relieved. The infiltration of CD4+ and CD8+ T cells and the sera level of IFNª²¦Ã and TNFª²¦Á were decreased significantly(P<0.05), whereas ILª²4 level in the supernatant of cultured lymphocytes was increased (P<0.05). CONCLUSION: Certain therapeutic effect of BMSC transplantation on EAN was observed. BMSCs could reverse the disbalance of Th1/Th2 cells by regulating the cytokine expression and could inhibit the activation and proliferation of T cells. ......