Preparation and preliminary application of monoclonal antibody against ¦Áª²zearalanol

    WEI Wanª²gui1, WANG Li1, BAI Yunª²feng, GONG Yi1, GU Ming2, ZHU Jian2, HAN Wei2, GUAN Rong2

    1Shanghai CASB Biotechnology CO., LTD, Chinese Academy of Sciences, Shanghai 200233, 2Shanghai Entryª²Exit Inspection and Quarantine Bureau of the Peoples Republic of China, Shanghai 200135, China

    \[Abstract\] AIM: To prepare monoclonal antibody (mAb) against ¦Áª²zearalanol (¦Áª²ZER) and develop an immunoassay for the detection of ¦Áª²ZER and its analogues residues in food derived from animal tissues. METHODS: ¦Áª²ZER was conjugated to BSA as immunogen to immunize BALB/c mice and mAb were prepared by hybridoma technique. mAb¡¯s characteristics (titer, Ig subclass, specificity and relative affinity) were identified by ELISA. Standard inhibitive cure was made and sensitivity of the mAb was identified. 37 samples derived from animal liver were detected for ¦Áª²ZER residues by competitive ELISA established in the study. RESULTS: 8 hybridoma cell lines stably secreting antiª²¦Áª²ZER mAb were obtained. The titer of one of them(4E5)was 5.142¡Á107.The Ig subclass was IgG1.The mAb was specific for ¦Áª²ZER and its analogues and had no crossª²reactivity with other compounds.8 positive results were found from the 37 samples derived from animal liver which were negative detected by HPLC. CONCLUSION: Antiª²¦Áª²ZER mAb has been prepared successfully. A rapid method using the mAb for detecting ¦Áª²ZER and its analogues residues in animal tissues has been established. ......