人用H5N1禽流感疫苗免疫动物效果观察
禽流感疫苗；免疫动物;中和抗体；效力试验,,禽流感疫苗；免疫动物;中和抗体；效力试验,1材料与方法,2结果,3讨论,参考文献
摘要： 目的 将用禽流感毒种R1203株按3种生产工艺生产的疫苗进行动物接种,观察疫苗的免疫效果。方法 用不同剂量的3种工艺生产的禽流感疫苗，分别免疫大鼠和家兔，肌肉接种2针(隔14d)，首针后14和28d静脉采血，测定接种1针和2针后，动物血清中血凝抑制抗体和中和抗体的产生情况。结果 大鼠的抗体反应较家兔敏感；大鼠和家兔在疫苗接种1针后14d,都能产生血抑抗体,滴度可达1∶40,但中和抗体滴度都很低,几乎测不到；接种2针后,血抑抗体或中和抗体反应都显著高于第1针；裂解-2苗接种大鼠和家兔后的抗体反应普遍高于另2种疫苗,且家兔量-效反应明显。结论 裂解-2疫苗优于其他2种；中和抗体能正确反应疫苗的质量；H5N1禽流感疫苗的效力试验可用于免疫家兔测定中和抗体和临床前研究的动物试验，用15μg血凝素(HA)接种家兔2针，血清中和抗体滴度达1:40或以上。关键词： 禽流感疫苗；免疫动物;中和抗体；效力试验
Observation on effect of avian influenza (H5N1) vaccine with immunization animals
ZHU Zhiyong,DING Xiaohang,ZHU Hanping,et al.
Key Lab of Vaccine against Hemorrhagic Fever with Renal Syndrome, Center for Disease Control and Prevention, Zhejiang Province (Hangzhou 310009, China)
Abstract： Objective The vaccines produced according to the three techniques with the virus seeds R1203 of H5N1 avian influenza were used to immunize animals and to observe the effect.Methods Rats and rabbits were immunized with the different dose of the three vaccines of avian influenza (H5N1) by an intramuscular injection twice (interval 14 days). Blood was collected intravenously in the day of 14 and 28. The hemagglutination inhibition antibody and neutralization antibody occurred in the serum of the animals after the first and second inoculation.Results The antibody reaction in the rats was more sensitive than the rabbits. The rats and rabbits both could produce hemagglutination inhibition antibody in 14th day with the titer of the antibody reaching at 1:40 while the titer of the neutralization antibody was very low and hardly detected. The second inoculation were significantly higher than the first inoculation. The antibody reaction after inoculation of the split2 vaccine in the rats and rabbits were significantly stronger than that inoculated with another two vaccines. The quantumeffect reaction was distinct in the rabbits. Conclusion The production technique of split2 vaccine was better than the other two. The neutralization antibody can accurately reflect the quality of vaccines. The efficiency test should be added in vaccines research and production with the method of immune rabbits. It is suggested to immunize rabbits twice with 12μg HA with the titer of the seraneutralization antibody reaching at 1:40 or over in animal test before clinical trial. ......
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