摘要：目的考察乙醇回流提取法提取黄连中盐酸小檗碱时主要影响因素。方法通过正交实验法确立试验体系，采用荧光分析法测定盐酸小檗碱含量。结果选择不同的提取条件盐酸小檗碱提取率有显著差异。结论 最优提取工艺是：50%乙醇溶液、8倍体积、85℃回流提取2次，每次2 h。

    关键词：黄连； 盐酸小檗碱； 提取工艺； 正交实验设计； 荧光分析法

    The Studies of Extraction Process of Berberine Hydrochloride in Coptis Chinensis Franch.

    WU Jia, TAN Gui-lian, YANG Hong

    (Yanjing Medical College, Capital University of Medical Sciences, Beijing,101300,China)

    Abstract：ObjectiveTo study the major influential factors of extraction by alcohol flowing on berberine hydrochloride in Coptis Chinensis Franch. MethodsTo establish the experiment system by orthogonal experimental design and adopt fluorometry to determine the content of berberine hydrochloride.ResultsWe observed the significant difference of the extraction rate of berberine hydrochloride under the different extraction conditions.Conclusion The optimum extraction process was achieved: the addition of 50% alcohol( 8 times raw herb in volume) into materials, refluxing and extracting for 2 times at 85℃. ......