反相高效液相色谱法测定夏枯草中两种三萜酸的含量
夏枯草；,乌索酸；,齐墩果酸；,反相高效液相色谱法,,夏枯草；,乌索酸；,齐墩果酸；,反相高效液相色谱法,1仪器与材料,2方法与结果,3讨论,参考文献：
摘要：目的建立同时测定夏枯草中乌索酸及齐墩果酸含量的方法。方法采用反相高效液相色谱法。色谱柱为 Nucleodur C18柱(4.6 mm×250 mm，5 μm)，流动相为甲醇:水(86∶14)，流速为0.8 ml/min，检测波长为210 nm，柱温25 ℃。结果乌索酸进样量在0.425 2 ～3.826 8 μg，齐墩果酸进样量在0.127 2 ～1.144 8 μg范围内，线性关系良好，乌索酸、齐墩果酸的加样平均回收率分别为98.0 %和98.7 %，RSD分别为1.0 %和1.4 %。结论方法简单准确，重现性好，适用于夏枯草药材的质量控制。关键词：夏枯草； 乌索酸； 齐墩果酸； 反相高效液相色谱法
Determination of Two Triterpene Acids in Prunella vulgaris L. by RP-HPLC
ZOU Sheng-Qin, JIANG Bai-quan, CHEN Wu
(School of Environmental Science and Engineering, Nanchang University, Nanchang 330029, China; Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Bioengineering Research Institute of Yichun University, Yichun 336000, China)
Abstract：ObjectiveTo develop a new method for simultaneous determination of ursolic acid and oleanolic acid in Prunella vulgaris L.MethodsThe high performance liquid chromatography was used.The column was Nucleodur C18 (4.6 mm×250 mm ......
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