桔梗；_蛋白质；_同工酶；_聚丙烯酰胺凝胶电脉_

河南不同产地与年限桔梗的聚丙烯酰胺凝胶电泳分析

http://www.100md.com 《时珍国医国药》 2007年第1期

桔梗；,蛋白质；,同工酶；,聚丙烯酰胺凝胶电脉,,桔梗；,蛋白质；,同工酶；,聚丙烯酰胺凝胶电脉,1仪器与材料,2方法与结果,3讨论,参考文献：

     摘要：目的鉴定河南省内不同产地与年限的桔梗药材。方法利用垂直平板聚丙烯酰胺凝胶电泳技术，作蛋白质、过氧化物酶同工酶、酯酶同工酶分析。结果各样品的蛋白质电泳图谱基本一致，同工酶酶谱有共有带，但酶带数量与活性强度却有差异。结论 PAGE分析快速、灵敏、技术简单，可用于桔梗药材的鉴定与质量评价。

    关键词：桔梗；蛋白质；同工酶；聚丙烯酰胺凝胶电脉

    PAGE Analysis for Radix Platycodon Grandiflorum in Various Ages from Different Places of Henan

    LI Xifeng, XUE Qiuping, DONG Chengming, XU Min

    (Henan College of TCM, Zhengzhou 450008,China)

    Abstract：ObjectiveTo distinguish Radix Platycodon grandiflorum in various ages from different places.MethodsThe protein, peroxidase isozyme and esterase isozyme of Radix Platycodon grandiflorum were studied by means of polyacrylamide gel electrophoresis.ResultsThere was little difference to a certain extent on their protein PAGE;the zymogram of the peroxidase isozyme and esterase isozyme of different Radix Platycodon grandiflorum consisted of common bands,but they had more or less difference in the number of isozymic bands and in their activity intensity. ConclusionPAGE has many advantages such as fast,high sensitivity and simple operation.It can be used for identification and quality evaluation of Radix Platycodon grandiflorum. ......

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