鼻渊枢滴鼻剂；_阿魏酸；_反相高效液相色谱法_

反相高效液相色谱法测定鼻渊枢滴鼻剂中阿魏酸的含量

http://www.100md.com 《时珍国医国药》 2007年第2期

鼻渊枢滴鼻剂；,阿魏酸；,反相高效液相色谱法,,鼻渊枢滴鼻剂；,阿魏酸；,反相高效液相色谱法,1仪器与试药,2方法与结果,3讨论,参考文献：

     摘要：目的建立以反相高效液相色谱法测定鼻渊枢滴鼻剂中阿魏酸含量的方法。方法采用反相高效液相色谱法，色谱柱为Diamonsil C18柱(200 mm×4.6 mm,5 μm)；甲醇水乙酸(35∶65∶1 V/V/V)为流动相；流速 1.0 ml/min；检测波长为 322 nm；外标法定量。结果阿魏酸在0.208~1.040 μg 范围内进样量X(μg)与峰面积Y呈良好的线性关系，Y=1 704 575.96 X-13 177.4(r=0.999 7，n=5)，平均回收率为97.12%，RSD=1.28%。结论反相高效液相色谱法测定滴鼻剂溶液中阿魏酸含量用于制剂质量控制，方法简便，灵敏，准确。

    关键词：鼻渊枢滴鼻剂；阿魏酸；反相高效液相色谱法

    Determination of the Content of Ferulic Acid in Biyuanshu Nose Drops by RPHPLC

    LI Chunhua,YAN Yuping,SONG Xiaoyu,FAN Jinghua

    (Traditional Chinese Medical School,Hebei Medical University, Shijiazhuang 050091,China; Graduate of Chinese Traditional Medical Department of Hebei Medical University in 2006, Shijiazhuang 050091, China)

    Abstract：ObjectiveTo establish the RPHPLC method for content determination of ferulic acid in Biyuanshu nose drops.MethodsThe assay was performed on a diamonsil C18 column by UV detection at the wavelength of 322 nm with methanolwateracetic acid (35∶65∶1)as the mobile phase at the flow rate of 1.0ml/min.ResultsThe content of ferulic acid was linear with its area over the range from 0.208 μg~1.04 μg ......

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