锰对人脐静脉内皮细胞及半胱天冬酶表达影响
锰;人脐静脉内皮细胞;凋亡;半胱天冬酶3(caspase3);半胱天冬酶8(caspase8),,锰;人脐静脉内皮细胞;凋亡;半胱天冬酶3(caspase3);半胱天冬酶8(caspase8),1材料与方法,2结果,3讨论,参考文献
摘要： 目的 探讨锰对人脐静脉内皮细胞系HUVEC-304细胞生长周期及半胱天冬酶3(caspas3)、半胱天冬酶8(caspase8)表达的影响。方法 以100～800μmoml/L)的氯化锰分别处理HUVEC-304细胞24～72h后，四甲基偶氮噻唑蓝(MTT)法检测EVC-304细胞的生长活性；流式细胞仪(FCM)检测细胞凋亡情况；蛋白印迹法(Western blot)检测HUVEC-304细胞在，氯化锰半数抑制浓度(IC50)400μmol/L作用24h时caspase8、caspase3的表达。结果 氯化锰可呈时间及剂量依赖性抑制HUVEC-304细胞的增殖和诱导细胞凋亡,抑制率为2234%～9094%，凋亡率为1020%～9873%。氯化锰24hIC50约为400μmol/L，在此浓度下，HUVEC-304细胞caspase8、caspase3表达显著增高,(P<005)。结论 氯化锰能够抑制HUVEC-304细胞的增殖，呈明显的时效和量效关系。caspase8、caspase3表达增高在细胞增殖和凋亡中起重要的作用，可能是其作用机制之一。关键词： 锰;人脐静脉内皮细胞;凋亡;半胱天冬酶3(caspase3);半胱天冬酶8(caspase8)
Effect of manganese on growth of HUVEC-304 cells and expression of caspase
GUO Xiaopeng,HUANG Guoxiang,WU Qing,et al.
Department of Preventive Medicin ,Medical College,Wuhan University of Science and Technology,(Wuhan 430080,China)
Abstract： Objective To investigate the effect of manganese on growth of HUVEC-304 cells and the expression of caspase8、caspase3.Methods HUVEC304 cells were given with various concentrations of MnCl2.The inhibitory ratio was measured by MTT assay.Apoptosis was observed by flow cytometry(FCM).The expression of caspase3 and caspase8 was measured by Western blot.Results MTT results revealed that manganese chloride (from 100 to 800μmol/L) might suppress the proliferation of HUVEC-304 cells(inhibitory ratio were 22.34%～90.94%) and induce apoptosis (apoptosis ratio were 10.20%～98.73%) in dose and time-dependent manner.The result of Western Blot showed that manganese inhibited caspase3 and caspase8 expression (P<0.05).Conclusion Manganese can inhibit proliferation of HUVEC-304 cells with time-and dose-dependent manner.These may be one of important mechanisms that manganese suppressed the proliferation of HUVEC-304 cells and induced apoptosis. ......
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