食品中单增李斯特菌PCR检测方法建立与评价
食品；单核细胞增生李斯特菌；聚合酶链式反应,,食品；单核细胞增生李斯特菌；聚合酶链式反应,1材料与方法,2结果,3讨论,参考文献
摘要： 目的 建立单核细胞增生李斯特菌(Listeria monocytogenes，LM)快速、敏感、特异的PCR诊断方法。方法 采用聚合酶链式反应技术(PCR)特异性扩增单核细胞增生李斯特菌溶血素基因(hlyA)，并评价该方法的特异性与敏感性。结果 在706bp处出现nuc基因的目的片断，只有单增李斯特菌的目的片段获得扩增， 其他菌种扩增均呈阴性；该方法可以检测到33ng/L的DNA。结论 PCR方法比传统细菌检测方法更特异、快速、灵敏和简便，为食品中单增李斯特菌的快速检测提供了新的手段。关键词：食品；单核细胞增生李斯特菌；聚合酶链式反应
Establishment of PCR method for detection of listeria monocytogenes in food
LIU Guihua, QIAO Feng, HUANG Xin，et al
Jilin Center for Disease Control and Prevention (Changchun 130062 China)Abstract： Objective To establish a rapid,sensitive and specific polymerase chain reaction (PCR) method for detection of Listeria monocytogenes (LM).Methods Polymerase chain reaction (PCR) technique was used to amplifiy the hlyA gene of listeria monocytogenes of food specifically ,value the specificity and sensitivity of this method.Results The target gene nuc was detected at 706bp ,and only the target gene of Listeria monocytogenes was amplified while the other bacteria was not detected;this method could also detect the DNA of 3.3ng/L.Conclusion The PCR method is more concise,rapid ,sensitive and more specific than conventional culture method for detection of Listeria monocytogenes in food. This method is useful for the investigation of listeria in food ......
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