紫外分光光度法；_决明子；_蒽醌____

决明子中蒽醌类成分的含量测定

http://www.100md.com 《时珍国医国药》 2007年第1期

紫外分光光度法；,决明子；,蒽醌,,紫外分光光度法；,决明子；,蒽醌,1仪器与材料,2方法与结果,3讨论,参考文献：

     摘要：目的建立决明子中蒽醌类成分的含量测定方法。方法采用聚酰胺吸附纯化样品，醋酸镁显色，紫外分光光度法测定。结果在4.6~18.4 μg/ml浓度范围内对照品1，8-二羟基蒽醌的吸收度与浓度线性关系良好，相关系数r＝0.999 8。平均回收率为101.0%，RSD＝2.48%(n＝9)。结论该法快速，灵敏，重现性好，能准确测定决明子中蒽醌类成分的含量。

    关键词：紫外分光光度法；决明子；蒽醌

    Determination of the Content of Anthraquinones in Semen Cassiae by Ultraviolet Spectrophotometry

    ZHANG Xiaomei, YANG Rongping, LI Na, WANG Binhao, LIANG Xuming

    ( Chongqing Academy of Chinese Materia Medica，Chongqing 400065, China)

    Abstract：ObjectiveTo estabish a method for the determination of the content of anthraquinones in Semen Cassiae.MethodsSample was purified by polyamide,colored with magnesium acetate and it's content was determined by ultraviolet spectrophotometry.ResultsThe absorbency and concentration of 1 ......

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