丁香；_总黄酮；_可见光分光光度法；_紫外分光光度法

可见光及紫外光分光光度法测定丁香总黄酮含量的研究

http://www.100md.com 《时珍国医国药》 2007年第2期

丁香；,总黄酮；,可见光分光光度法；,紫外分光光度法,,丁香；,总黄酮；,可见光分光光度法；,紫外分光光度法,1仪器与试剂,2方法,3结果,4结论,参考文献：

     摘要：目的研究可见光及紫外分光光度法测定丁香总黄酮含量的方法。方法可见光分光光度法是以芦丁为参照品，硝酸铝作显色剂，在510 nm波长测定总黄酮含量。紫外分光光度法是以芦丁为参照品，在359 nm波长测定总黄酮含量。结果可见光法平均回收率是99.46% , 紫外光法是99.13%。RSD分别是1.15 % 和0.90%。结论紫外分光光度法是一种较理想的测定方法。

    关键词：丁香；总黄酮；可见光分光光度法；紫外分光光度法

    Study on the Determination of Flavonoid in Eugenia caryophyllata Thunb. by VIS and UV Spectrophotometry

    LI Yu, LING Weizhong, YE Yong， LI Meidi, LAN Fangcun, XIAO Ailing, ZHENG Kangwei， HUANG Guochao, HUANG Yi

    (Department of Chemical Engineering, Guangdong Industrial Technological College,Guangzhou 510300,China; School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275,China; Zhanjiang Admixture Factory,Zhanjiang 524018,China)

    Abstract：ObjectiveTo determine total flavonoid content in Eugenia caryophyllata Thunb. by VIS and UV Spectrophotometry.MethodsThe contrast sample of VIS Spectrophotometry was rutin, the revealing agent was Al(NO3)3 and the analyzed wavelength was 510 nm. The contrast sample of UV Spectrophotometry was rutin and the analyzed wavelength was 359 nm.ResultsThe average recoveries of VIS and UV Spectrophotometry were 99.46% and 99.13%.The RSD were 1.15 % and 0.90%.ConclusionUV Spectrophotometry is a gooel method for detection of total flavonoid content in Eugenia caryophyllata Thunb. . ......

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