圣和散对脑胶质瘤细胞诱导分化的研究
圣和散；,脑胶质瘤；,分化,,圣和散；,脑胶质瘤；,分化,1材料与方法,2结果,3讨论,参考文献：
摘要：目的观察圣和散对脑胶质瘤C6细胞株体外生长及诱导分化的作用。方法用圣和散处理脑胶质瘤C6细胞，采用甲基噻唑基四唑(MTT) 比色法检测圣和散对C6 细胞增殖的抑制作用, 以免疫组化SABC法检测胶质纤维酸性蛋白(GFAP)和c-myc表达。结果圣和散可显著抑制 C6 细胞体外增殖，并呈现时间、剂量依赖性。在终浓度为5 mg/ml和10 mg/ml时,对 C6 增殖的抑制率达 (49.62±1.13) %和 (69.38±1.42) %，明显优于对照组(P<0.01)。免疫组化可见 SHP组较对照组 GFAP 表达明显增强，c-myc表达下降。结论圣和散对胶质瘤 C6 细胞系的增殖有明显的抑制作用，能通过诱导分化降低脑胶质瘤细胞恶性程度。关键词：圣和散； 脑胶质瘤； 分化
Inducing Differentiation Effect of Shenghe Powder on C6 Brain Glioma Cells
XIA Yuesheng, WANG Jianhua, WU Yongzhong, WANG Huan
(Fifth Staff Hospital of PLA,Shanxi 043801，China; Tangdu Hospital of Fourth Military Medical University, Xi'an, 710038, China; Shanxi TCM College, Taiyuan 030024, China)
Abstract：ObjectiveTo investigate the effects of Shenghe Powder(SHP) on the proliferation and inducing differentiation of brain glioma C6 cell line in vitro.MethodsBrain glioma C6 cell line was co-cultured with SHP.Methyl thiagoly tetragolium (MTT) was used to measure the level of the proliferation of C6 cultured with SHP.The expressions of glial fibrillary acidic protein (GFAP) and c-myc of C6 cells were detected by immunohistochemical SABC method. ResultsThe proliferation of C6 was obviously inhibited by SHP in a concentration- and time-dependent manner. The inhibitory rate was (49.62±1.13) % and (69.38±1.42) % in SHP at 5mg/ml and 10 mg/ml,compared with the control group, there was a significant difference(P<0. 01) .The expression of GFAP was obviously enhanced and c-myc decreased significantly in C6 cells cultured with SHP.ConclusionSHP can inhibit the proliferation of C6 and decrease malignant degree by inducing differentiation of the C6 cells . ......
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