霍乱弧菌；噬菌体；序列分析_

霍乱弧菌新类型溶源性噬菌体基因组结构研究

http://www.100md.com 《中国公共卫生》 2007年第4期

霍乱弧菌；噬菌体；序列分析,,霍乱弧菌；噬菌体；序列分析,1材料与方法,2结果,3讨论,参考文献

     摘要：目的研究霍乱弧菌埃尔托型(EVC)新类型溶源性噬菌体(CTXφ)和核心区不含ctxAB基因的溶源性噬菌体(nct-CTXφ)基因组的结构。方法采用基因扩增、测序及序列生物信息学等方法进行观察与分析。结果在我国EVC中，存在仅携带古典型(class)nct-CTXφ、CTXφ或埃尔托型(ET)CTXφ基因组类型，并发现nct-CTXclassφ和CTXETφ共存、nct-CTXnewETφ和CTXclassφ共存的新类型。CTXφ和nct-CTXφ基因组zot基因3′末端，60bp序列中40%位点不同，推测的氨基酸序列中60%位点不同。class、ET和newET类型ig1、rstR和ig2基因序列同源性分别为770%～974%，104%～179%，96%～648%，RstR同源性92%～241%。CTXφ基因组均位于染色体TLC基因簇附近。上述不同类型EVC的tcpA、TcpA、ctxA、ctxB、CtxA和CtxB与GenBank中EVC和O139群产毒株的同源性分别>99%，99%，99%，98%，98%和96%。结论首次在EVC中发现nct-CTXclassφ和CTXETφ、nct-CTXnewETφ和CTXclassφ共存的新类型。

    关键词：霍乱弧菌；噬菌体；序列分析

    Study on structures of new types of CTXφ and nct-CTXφ genomes

    RUI Yongyu,KAN Biao,GAO Shouyi,et al.

    Department of Laboratory Medicine,Nanfang Hospital,Southern Medicine University(Guangzhou 510515,China)

    Abstract：Objective To study on structures of new types of CTXφ and nct-CTXφ genomes of EI Tor biotype vibrio cholerae.Methods PCR,sequencing and sequence analysis were conducted.Results Only nct-CTXclassφ,CTXclassφ,or CTXETφ genome type,nct-CTXclassφ and CTXETφ,nct-CTXnewETφ and CTXclassφ genomes coexisted types in EI Tor strains isolated from China were found.Different rates of last 60 bp of zot gene and Zot of CTXφ and nct-CTXφ were 40% and 60%.Identities of ig1,rstR,ig2 gene and RstR of class、ET and newET types were 77.0%～97.4%,10.4%～17.9%,9.6%～64.8%,and 9.2%～24.1% differentially.Different types of CTXφ genome were found near TLC cluster.Identities of tcpA,TcpA,ctxA,ctxB,CtxA,and CtxB of strains in this study with others toxic EVC and O139 VC were over 99%,99%,99%,98%,98%,and 96%.Conclusion nct-CTXclassφ and CTXETφ,nct-CTXnewETφ and CTXclassφ genomes coexisted types in EI Tor were found first. ......

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