黄芩；_蒺藜；_罗布麻；_淋巴细胞；_巨噬细胞_

11种中药对小鼠淋巴细胞活性及腹腔巨噬细胞功能的影响

http://www.100md.com 《时珍国医国药》 2007年第2期

黄芩；,蒺藜；,罗布麻；,淋巴细胞；,巨噬细胞,,黄芩；,蒺藜；,罗布麻；,淋巴细胞；,巨噬细胞,1材料,2方法,3结果,4讨论,参考文献：

     摘要：目的研究具有免疫调节作用的11种中药水提取物，对小鼠脾淋巴细胞增殖活性以及腹腔巨噬细胞吞噬功能的影响。方法采用体外检测不同浓度的11种中药水提取物对正常、ConA及LPS诱导的小鼠脾淋巴细胞增殖作用的影响，运用中性红法检测巨噬细胞的吞噬功能。结果黄芩水提取物对正常淋巴细胞的增殖活性无明显影响，对ConA及LPS诱导的脾淋巴细胞增殖活性有显著的抑制作用，其IC50分别为16.54 ，19.00 μg/ml；蒺藜及罗布麻水提取物能够显著促进巨噬细胞吞噬功能。结论黄芩水提取物对小鼠脾淋巴细胞增殖活性具有一定的调节作用，蒺藜及罗布麻对巨噬功能有一定的调节作用。

    关键词：黄芩；蒺藜；罗布麻；淋巴细胞；巨噬细胞

    Effect of 11 Traditional Chinese Herbs on Proliferation of Splenic Lymphocyte and Function Phagocytosis of Peritoneal Macrophage

    SHENG Juan，HONG Tie*

    (Department of Pharmacology, Pharmacy College of Jilin University, Changchun 130021, China; Academy of Traditional Chinese Medicine and Materia Medica of Jilin Province , Changchun 130021, China)

    Abstract：ObjectiveTo investigate the immune modulatory effect of water extract of 11 traditional Chinese herbs on the proliferation of splenic lymphocyte and function phagocytosis of peritoneal macrophage. Methods The pattern of concanavalin A (ConA) or lipopolysaccharide(LPS) induced proliferative response of murine lymphocytes was assessed. Cell proliferation was measured by the XTT (2,3bis[2 4nitro5sulfophenyl]-2H-tetrazolium5carboxanilide assay in the normal cells and the cells treated with ConA or LPS, then measured the proliferation after treated with water extract of 11 traditional Chinese herbs( The final concentration of water extract of 11 traditional Chinese herbs: 100.0 ......

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