固相萃取；_高效液相色谱；_黄芪甲苷____

固相萃取高效液相色谱法测定黄芪中黄芪甲苷的含量

http://www.100md.com 《时珍国医国药》 2007年第1期

固相萃取；,高效液相色谱；,黄芪甲苷,,固相萃取；,高效液相色谱；,黄芪甲苷,1仪器与材料,2方法,3讨论,参考文献：

     摘要：目的采用固相萃取高效液相色谱方法分析黄芪中黄芪甲苷的含量。方法采用C18固相萃取小柱纯化黄芪提取物中的黄芪甲苷，反相高效液相色谱—紫外检测方法测定黄芪中黄芪甲苷含量。色谱柱为Zorbax SBC18柱(4.6 mm×150 mm，5 μm)，以乙腈水(32∶68)为流动相，流速1.0 ml/min，柱温30℃，检测波长200 nm。结果黄芪甲苷进样量在1.408～7.04 μg范围内与峰面积呈良好线性关系，r=0.999 9。平均回收率为101.5%。结论该方法操作简便，重现性好，可作为黄芪药材或其它含黄芪制剂中黄芪甲苷含量的分析方法。

    关键词：固相萃取；高效液相色谱；黄芪甲苷

    Determination of Astragaloside IV in Radix Astragali by SPEHPLC

    YANG Kedi， LI Hong， LONG Yunfei， ZHENG Zhihui， WAN Shungang

    (School of Chemistry and Chemical Engineering, Guangxi University，Nanning 530004，China)

    Abstract：ObjectiveTo establish SPEHPLC method for the determination of astragaloside IV in Radix Astragali. MethodsThe astragaloside IV in sample was purified with C18 solid phase column and its content was determined with HPLCUV method. The astragaloside IV was separated on a Zorbax SBC18 column (φ4.6 mm×150 mm, 5 μm) with acetonitrile-water (32∶68, v/v) as mobile phase, and the detective wavelength was set at 200nm. ResultsThe linear range was 1.408～7.04 μg (r=0.999 9). The average recovery of astragaloside IV was 101.5%. ConclusionThe method is simple, accurate and can be used for the quality control of Radix Astragali and its preparation. ......

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