摘要 目的：研究COL4A2基因多态性与中国农村男性骨密度关系。方法：采用双能X线吸收仪对367名男性研究对象进行腰椎及股骨扫描，应用PCR-限制性片段长度多态性(PCR-RFLP)方法检测COL4A2基因Gly683Ala多态位点基因型，用广义线性模型分析此位点多态性与腰椎2~4及股骨骨密度关系。结果：在调整环境危险因素前后，均未显示COL4A2基因Gly683Ala多态性与股骨颈及腰椎2~4骨密度有显著相关性(P＝0.328、0.813)。结论：在中国农村男性人群中COL4A2基因Gly683Ala多态性与股骨颈及腰椎2~4骨密度可能无相关性。

    关键词 Ⅳ型胶原α2链(COL4A2)；多态性；相关性；骨矿物质密度(BMD)

    The Relationship Between Gly683Ala Polymorphism of COL4A2 Gene and Bone Mineral Density in Chinese Rural Men

    WU Di, HONG Xiumei, YANG Tianpeng，LI Zhiping, ZANG Tonghua

    (Anhui Province Institute of Biomedicine, Anhui Medical University, Hefei 230032,China)

    Abstract Objective：In order to investigate the association of polymorphism of collagen type Ⅳ alpha 2 gene (COL4A2) with Bone Mineral Density (BMD) in Chinese rural men. Methods：The bone mineral density was measured at the lumbar spine and femur using dual-energy X-ray absorptiometry in 367 study subjects. PCR-restriction fragment length polymorphism (PCR-RFLP) was used to examine Gly683Ala polymorphism of COL4A2 gene. Generalized linear model was used to analyse the association of this polymorphism with the bone mineral density of the lumbar spine (L2-4) and femur. Results：Without or with adjustment for environmental covariates, no significant correlation was found between the Ala683Ala polymorphism of the COL4A2 gene and BMD, including femoral neck, lumbar spine (L2-4) (P=0.328、0.813 respectively).Conclusion：There is no significant relationship between the Gly683Ala polymorphism of the COL4A2 gene and BMD of femoral neck and lumbar spine (2-4) in Chinese rural men. ......