砷；肝；8Nitroguanine；免疫组化；核酸损伤

砷暴露小鼠肝组织核酸损伤免疫组织化学观察

http://www.100md.com 《中国公共卫生》 2007年第4期

砷；肝；8Nitroguanine；免疫组化；核酸损伤,,砷；肝；8Nitroguanine；免疫组化；核酸损伤,1材料与方法,2结果,3讨论,参考文献

     摘要：目的为进一步研究砷对肝脏的毒性作用机制提供实验依据。方法昆明种小鼠40只，随机分为4组：1,2,4mg/L三氧化二砷染毒组和生理盐水组，连续染毒60d，取小鼠肝组织固定，用免疫组化方法观察肝脏组织细胞的8Nitroguanine表达。结果染砷组小鼠肝细胞出现抗8Nitroguanine免疫染色阳性反应，并随着砷暴露剂量的增加而呈现明显的剂量-反应关系。结论慢性低剂量砷暴露可诱发小鼠肝细胞8Nitroguanine的高表达，提示8硝基鸟嘌呤核苷(8Nitroguanine)可以作为砷对肝细胞毒性作用的早期敏感生物标志物。

    关键词：砷；肝；8Nitroguanine；免疫组化；核酸损伤

    Observation on nucleic acid damage of liver in mice exposed to arsenic in low dose by immunohistochemistry

    LI Qiujuan,YANG Guang,YE Jianxin,et al.

    Department of Hygiene,Dalian Medical University(Dalian 116027,China)Abstract： Objective To provide evidence for exploring the mechanism of arsenicinduced hepatoxicity.Methods Forty mice were diveded into 4 groups, e.g.,one controls and 3 experimental groups(1 mg/L As2O3,2 mg/L As2O3 and 4 mg/L AS2O3).As2O3 was administered for 60 days.Immunohistochemistry method was used to investigate 8Nitroguanine expression in liver tissue of mice.Results Expression of 8Nitroguanine was observed in the liver tissue of mice exposed to arsenic and there was relationship of doseresponse in expression of 8Nitroguanine with the increase arsenic dose.Conclusion Arsenic induced high expression of 8Nitroguanine in liver tissue of mice.8Nitroguanine might be an earlier sensitive bio-marker for arsenic toxicity to liver. ......

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