石榴根皮等9种中药体外抗人芽囊原虫的效果观察
人芽囊原虫；,石榴根皮；,胡黄连；,杀虫效果；,形态,,人芽囊原虫；,石榴根皮；,胡黄连；,杀虫效果；,形态,1材料,2方法,3结果,4讨论,参考文献：
摘要：目的探讨石榴根皮等9种中草药杀人芽囊原虫的效果。方法采用不同浓度中草药水提液进行体外抗人芽囊原虫实验，并用光镜观察作用后人芽囊原虫的形态变化。结果随着药物作用时间的延长和药物浓度的增加，石榴根皮、胡黄连、黄连、苦楝皮对人芽囊原虫有不同程度的凋亡现象，这些药物对人芽囊原虫的MIC值分别为 0.1，0.6，0.8，0.8 g/ml，光镜观察虫体表膜凹陷，剥脱，破裂。低浓度胡黄连药物作用虫体后均转变成包囊，而白头翁、南瓜子、使君子、槟榔、苦参对人芽囊原虫均无作用。结论 石榴根皮、胡黄连可直接作用于虫体表膜，具有较强的杀人芽囊原虫的作用。关键词：人芽囊原虫； 石榴根皮； 胡黄连； 杀虫效果； 形态
The Trichomnacidal Effect of Nine Chinese Herbs on the Blastocystis hominis
SU Shuilian, CHEN Guifeng*, ZHANG Ruiqi, XIE Qiongjun, LIAO Hua
(Gannan Medical College, Ganzhou, Jiangxi Province 341000,China)
Abstract：ObjectiveTo study the effect of Psidium guajaual and other eight Chinese herbs on the Blastocysitis hominis. MethodsTrchomonacidal effect of Chinese herbs was investigated using various concentration of Chinese herbs water extract against Blastocysitis hominis in vitro.The morphological changes of the Blastocysitis hominis were observed by light microscope.ResultsWith prolongation of the time of drug action and increase of its concentration, Psidium guajaul, Picrorhra scrophulariitlora Pennell, Coptis chiennsis Franch and Melia azedarach Linn have the effect of apoptosis to Blastocystis hominis. Their lowest concentrations were respectively 0.1,0.4,0.6,0.8g/ml.We can observe the cell membrance hollowed,exfoliated and ruptured. The Blastocystis hominis change into cyst with low concentration of Picrohica scrophulariitlora Pennell. And Pulsatilla chinenensis Bunge Regel, Cucurita moschata Ouch, Quisgualis indical, Areca actechata Duch and Sophora flavescens Aic have no effect to Blastocystis hominis.ConclusionPsidium guajaual and Picrorhica scrophulariitlora Pennell destroyed cell membrane directly and exhibited effective trichomonacidal Blastocystis hominis action. ......
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