甲状腺肿瘤；_寡核苷酸类_反义；_端粒酶逆转录酶_

端粒酶逆转录酶反义寡核苷酸对甲状腺癌细胞凋亡的影响

http://www.100md.com 《第四军医大学学报》 2007年第7期

甲状腺肿瘤；,寡核苷酸类,反义；,端粒酶逆转录酶,,甲状腺肿瘤；寡核苷酸类，反义；端粒酶逆转录酶,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effect of antisense oligonucleotides encoding telomerase reverse transcriptase on thyroid carcinoma cell apoptosis

    SHI Mei, YI Jun, LING Rui, GONG WeiDong, FU Li

    1Department of Radiotherapy

    2Department of General Surgery, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    3Department of Interventional Radiology, Tangdu Hospital, Fourth Military Medical University, Xian 710038, China

    【Abstract】 AIM: To investigate the effect of antisense oligonucleotides (ASODN) encoding telomerase reverse transcriptase (TERT) mRNA on thyroid carcinoma (TC) cell apoptosis. METHODS: The ASODN and sense oligonucleotides (SODN) encoding TERT were delivered respectively to the TC cells in vitro. The effect of ASODN encoding TERT mRNA on TC cell apoptosis was detected by transmission electron microscope, Hoechest 33258 staining and flow cytometry. RESULTS: Transmission electron microscope showed ASODN could induce typical apoptotic manifestations of TC cells; Hoechest 33258 staining showed ASODN could increase the number of apoptotic cells from 52±16 to 178±32; flow cytometry revealed that the apoptosis rates of TC cells treated by 5 μmol/L ASODN, SODN, or nothing were 10.4%, 1.6% and 0, respectively (P<0.05). CONCLUSION: ASODN complementary to TERT mRNA at some concentration can sequencespecifically enhance TC cell apoptosis. ......

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