Therapeutic effect of invigorating kidney on postmenopausal osteoporosis in rats

    WEI Yuª²Fang1, WANG Changª²Hai1, LIU Yuª²Lin2, ZHANG Shanª²Hong3, LIU Ya4, LI Feng 1

    1Department of Traditional Chinese Medicine, 3Department of Senile Diseases, 4Outpatient Department, Xijing Hospital, 2School of Basic Medicine, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To study the therapeutic effect of invigorating the kidney on postmenopausal osteoporosis in rats. METHODS: Thirtyª²two healthy female SD rats were randomly separated into 4 groups: normal group, model group, western medicine group, kidneyª²invigorating group, 8 rats in each group. The postmenopausal osteoporosis model was established in the latter 3 groups by ovariectomy. One week later, rats in western medicine group were drenched with 50 mg/L nilestriol, 5 mL/week, and with 5 mL isotonic sodium chloride in other time; ones in kidneyª²invigorating group were drenched with traditional Chinese medicine 200 g/L for tonifying the kidney, 5 mL/d, equivalent volume and concentration of sodium chloride was drenched every day in the same time to normal group and model group. Eight weeks later, blood from all the rats was harvested by removing eyeball, and left femoral bone was also taken. Bone mineral density (BMD) in femoral bone was measured by DPXª²Plus, and serum levels of E2 was detected by ELISA. RESULTS: In model group compared with other groups, BMD: normal vs model (P<0.01); western medicine vs model (P<0.01); kidneyª² invigorating vs model (P<0.01); E2: normal vs model (P<0.01)£» western medicine vs model (P<0.01)£» kidneyª²invigorating vs model (P<0.01); normal vs western medicine (P<0.01); normal vs kidneyª²invigorating (P<0.01). CONCLUSION: Invigorating the kidney with traditional Chinese medicine can increase BMD, serum E2 in ovariectomized rat models, thus exerting the therapeutic effect on postmenopausal osteoporosis. ......