Establishment of an orthotopic tracheal transplantation model with inbred mice

    ZHAO Jinª²Bo , LI Xiaoª²Fei, LIU Kun, ZHOU Yongª²An, LIANG Zhiª²Bo, CHENG Qingª²Shu

    Deparment of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China

    ¡¾Abstract¡¿ AIM£º To establish an orthotopic tracheal transplantation model with inbred mice. METHODS: Weightª²matched inbred mice with different germlines were randomly assigned to 2 experimental groups: A,C57BL/6(donator) ¡ú BALB/c(receptor)(n=10 pairs); B, BALB/c(donator) ¡úBALB/c(receptor)(n=10 pairs). Weightª²matched Kunming(KM) mice were assigned as a control group C: KM(donator)¡ú KM(receptor) (n=10 pairs).The orthotopic tracheal transplantations were performed according to the designed groups and the transplanted trachea was coated with the anterior cervical muscles for revascularization.Tracheal graft rejection was monitored by daily clinical airway assessment, histological examination using HE staining and scanning electron microscopy. RESULTS£º All the mice in group A and B were living until they were sacrificed, while 6 mice kept alive in group C. Tracheal autografts in group A and C had notable rejection reaction,but group B did not.In the 3 groups, the epithelial scores were 1.094¡À0.120,2.872¡À0.059 and 0.995¡À0.444; the numbers of lymphocyte were (20.18¡À1.31) /HPF, <5 /HPF and( 22.55¡À4.87) /HPF; the ratios of anuclear chondrocyte/osteocyte were (27.22¡À1.09)%, (10.60¡À1.01)% and (31.40¡À6.43)%. Various cellular indexes such as the epithelial score, the number of lymphocyte and the ratio of anuclear chondrocyte/osteocyte in group A and C showed marked statistical difference when compared with those of group B, but no statistical difference was found when group A was compared with group C. CONCLUSION£º A C57BL/6(donator) ¡ú BALB/c(receptor)orthotopic tracheal transplantation model has been established successfully.Various pathophysiological changes in the tracheal transplantation would well be reflected in both the inbred and closed population mice.The inbred model seems to be more stable than the closed population. ......