Antioxidation of Lycium barbarum extracts

    YANG Xueª²Kang£¬ HAI Chunª²Xu£¬ LIANG Xin£¬ ZHAO Haiª²Long£¬ BAI Hua

    Department of Toxicology£¬ School of Military Preventive Medicine£¬ Fourth Military Medical University£¬ Xi¬ðan 710033£¬ China

    ¡¾Abstract¡¿ AIM£º To investigate the antioxidative activity of the ethanol and water extracts of Lycium barbarum and their doseª²effect relationship. METHODS£º The Lycium barbarum extracts were provided by our laboratory. Spragueª²Dawley rats were killed and then the livers were harvested to isolate the microsomes by calcium precipitation. The oxidantª²scavenging potential of the water and alcohol extracts was measured using the following chemiluminescence systems: CoCl2/EDTAª²induced luminol chemiluminescence method and xanthineª²xanthine oxidaseª²luminol method. Two types of lipid peroxidation of rat liver microsomes models were used to determine the antioxidation of Lycium barbarum extracts. Then the content of malondialdehyde (MDA) was tested by colorimetry for analyzing the antioxidation of Lycium barbarum extracts in the 2 models. RESULTS£º In the 2 chemiluminescence systems, compared with control group, the inhibition rate of ¡¤OH and O2-¡¤ by different dosages of the 2 extracts significantly increased(P<0.01) ......