Apoptosis induction of human cervical cancer Ca Ski cells by hILª²24 gene

    SHI Hua, CHENG Haiª²En, ZHANG Yi, GUO Fengª²Jin, YI Faª²Ping, MA Yongª²Ping, SONG Fangª²Zhou

    Department of Biochemistry and Molecular Biology, Key Laboratory of Clinical Laboratory Diagnostics of Ministry of Education, Chongqing 400016, China

    ¡¾Abstract¡¿ AIM: To investigate the hILª²24 gene inducing apoptosis of human cervical cancer Ca Ski cells and its possible mechanism. METHODS: Ca Ski cells were transfected with hILª²24 eukaryotic expression vector pcDNA3.1(+)ª²hILª²24 by lipofectamine2000. The effect of hILª²24 on the transcription of HPV E6 gene was analyzed by RTª²PCR. The effect of hILª²24 on P53 protein was analyzed by Western Blot. Apoptosis of Ca Ski cells induced by hILª²24 gene was analyzed by FACS. RESULTS: Transcription level of HPV E6 gene decreased significantly, the amount of P53 protein increased significantly and the apoptosis rate of Ca Ski cells increased significantly after hILª²24 transfection. CONCLUSION: HILª²24 gene can inhibit the transcription of HPV E6 gene, recover the activity of P53 protein, then induce the apoptosis of Ca Ski cells. ......