Comparison of two embryo transfer methods in mice

    ZHAO Siª²Hai1,2, ZHENG Huaª²Dong1, CHU Yongª²Lie2, LIN Yan1, ZHANG Chunª²Fang1, CHEN Zhengª²Lan1, ZHU Hong1, YANG Pengª²Hui1, LIU Enª²Qi1, 3

    1Laboratory Animal Center, 2Department of Immunology and Microbiology, 3Key Laboratory of Ministry of Education of Environment and Genes Related to Diseases, Medical College, Xi¬ðan Jiaotong University, Xi¬ðan 710061, China

    ¡¾Abstract¡¿AIM: To promote the success rate of embryo transfer in mice by improving the traditional embryo transfer method. METHODS: Embryos were transferred to oviducts of 106 recipients by the traditional method (tearing the bursa open over the infundibulum of oviduct) or the improved method (puncturing a hole in bursa with embryo transfer pipette and blowing embrys into oviduct through the hole). ICR (n=31) and C57BL/6 (n=16) pseudopregnant mice were transferred embryos by the improved method and other ICR (n=39) and C57BL/6 (n=20) mice did by the traditional method. RESULTS: The improved method reduced the blooding of bursa and the followed inflammatory adherence between oviduct and body wall, so it could achieve a relatively higher success rate of embryo transfer. CONCLUSION: The improved embryo transfer method can raise the success rate of embryo transfer in mice. ......