ABSTRACTS OF ORIGINGAL ARTICLES
作者：
单位：
关键词：
中华医学杂志930646 Hepatitis B X antigen binding to p53 protein in the pathogenesis of primary hepatocellular carcinoma Zhu Minghua,et al.Department of Pathology,Fourth Military Medical University,Xi'an 710032
Much evidences have showed a close relation between HBV infection and the development of primary hepatocellular carcinoma (PHC).Molecular genetic study showed loss of heterozygosity in PHC and that point mutation of p53 exon 7 at codon 249 was important to PHC.But these changes of PHC associated with aflatoxin B1 and HBV were not apparent.Thirteen cases of PHC were examined to determine whether there were any telationship between HBxAg and p53 protein.HBV-DNA was integrated to PHC tissue in 9 of the 13 cases by southern blot;HBsAg,HBxAg or HBcAg was found in 8 by immunohistochemical staining.None had the point mutation of p53 exon 7 at codon 229,247 and 249 by PCR and restriction enzymes.Immunoprecipitation analyses were carried out to test whether anti-X antiobody was capable of precipitating p53 or anti-p53 could immunoprecipitate HBxAg polypeptides from HBV infected tissue.Specific band was detected at 53000 in 7 of the 13 cases as precipitating with anti-HBxAg and western blotting using anti-p53,and specific band was detected at 13000,17000 or 28000 in 6 of the 13 cases as precipitating with anti-p53 and western blotting using anti-HBxAg.These results revealed that the binding of HBxAg to p53 may be analogous to that observed in tumor antigen encoded from selected papovaviruses,and adenoviruses,and may be a crucial step in the development of PHC among most HBV infected patients.
, http://www.100md.com
Key words Genes,p53 Hepatilis B virus Genes,regulator hepatoma
(Original article on page 325)
Distribution of HBV in testicle tissue of patients with hepatitis B infection Lang Zhenwei et al.Beijing Youan Hospital,Beijing 100054
To study distribution of HBV in the testicle tissue,we used polyclonal antibody and ABC method in detecting autopsy testicle in 34 cases.The positive rate of HBsAg and HBcAg was 52.9% and 11.8% respectively.The positive cells of spermatogonium,primary spermatocytes,spermatid and sertoli cells were single or grouped in clusters form in the semimiferous tubule.Of the 6 cases studied with in situ hybridization,2 han HBV DNA in spematogenic cells in individual nucleus.The findings support morphologically the transmission of HBV via sexual contact.
, 百拇医药
Key words Hepatitis B virus Immun-oenzyme technics Testis
(Original article on page 329)
Molecular cloning and sequencing of HCV gene from human blood Tan Deming,et al.Department of Infectious Diseases,First Affiliated Hospital,Hunan Medical University,Changsha 410008
To clone and characterize hepatitis C virus strain in China,we extracted RNA from the pooled plasma of persons with HCV infection in Hunan,China.HCV gene was amplified and cloned by RT-PCR and gene recombinant techniques.A total of 2 307 nucleotides of the complementary DNA clones of HCV(HCV-Hun),including the clone of 5' noncoding region (311bp),core region (340bp),envelope region (397bp),NSI region (634bp),NS3 region (245bp),and NS5 region (380bp),NS3 region (245bp),and NS5 region (380bp) were isolated and identified.The homologies in nucleotide sequuence between HCV-Hun and HCV-US or HCV-J were 98.1% and 98.7% respectively in 5' noncoding region;92.6% and 97.4% in core region;74.3% and 88.7% in envelope region;83.5% and 91.0% in NS1 region;82.9% and 94.7% in NS3 region;and 74.8% and 90.1% in NS5 region.The similar results were seen in deduced amino acid sequence homologies between above HCV stains.These findinges indicate that HCV-Hun were more mimic to HCV-J than HCV-US,and there were considerable heterogeneity in hepatitis C virus genomes isolated from different areas of the world.
, http://www.100md.com
Key word Hepatitis C virus Recombination,Genetic Cloning,Molecular Immunoenzyme technics
(Original article on page 332)
Hereditable characteristics of attenuated live hepatitis A virus H2 strain Chai Shoaai,et al.Virus Research Institute,Zhejiang Academy of Medical Sciences,Huangzhou 310013
The toxicity of attenuated hepatitis A virus H2 strain was not increased in testing KMB 17 cells of 5 consective generations of monkeys at 36℃.After injectio of attenuated live hepatitis A virus H2 strain in humans,no abnormality was noted.Serum hepatitis A antigen was positive after 38 children aged 3～9 years were given oral 106.5 TCKD50,whereas it was 100% positive in the group of same age after percutaneous vaccination.Noninfection of oral administration is and important hereditable characteristic differentiation from wild strains.
, 百拇医药
Key words Hepatitis A virus Vaccine Hereditable characteristics
(Original article on page 335)
Mapping of the transforming gene by fluorescence in situ hybridization He Hong,et al.Tumor Research Institute,Hunan Medical University,Changsha 410078.
A transforming gene of human nasopharynageal carcinoma was cloned from Chinese nasopharyngeal carcinoma cell line CNE2.This gene was different from a number of known oncogenes as was reported previously.The NPC transforming gene was 16kb in size,and the Alu negative 2.8kb fragment was cloned into pSP70 plasmid.Linearized Tx pSP70 recombinant plasmid was nick translated in the presence of biotin-11-dUTP,incubated with an excess of sonicated human DNA,and used as a probe for fluorescence in situ hybridization.Signals of hybridization were detected by successive treatments with FITC-labeled avidin.57% of grains were located on chromosome 8;51.8% of them were mapped to the 8q23→8q24.1 region of chromosome 8's.The Tx gene,therefore,could be assigned to the band 8q23→8q24.1.
, 百拇医药
Key words Nucleic acid hybridization Chromosome mapping Nasopharyngeal neoplasms
(Original article on page 338)
Distribution of extracellular viral antigen of epidemic hemorrhagic fever in human autopsy cases Liu Yanfang, et al.Depqrtment of Pathology,Fourth Military Medical University.Xi'an 710032
Using improved immunohistochemical methods,repeated PAP and repeated PAP+ABC,we found in the extracellular spaces of autopsy cases of epidemic hemorrhagic fever large amount of viral antigens.Two forms of antigens were found:diffuse soluble antigen and inclusion body like granular.The former was mainly G2 protein and the latter was mainly Nproteon.They were found in serum,lymph,CSF,edema fluid,fluids in renal,semenal tubules,alveolar and bronchial spaces,peptic,intestinal,pancreatic and thyroidal secretions.In most patients,the diffuse antigens were accompanied by IgG and C1q.A large amount of antigens,were excreted from the patients.
, http://www.100md.com
Key words Hemorrhagic fever,epidemics Body fluids Antigens,viral
(Original article on page 341)
Effect of rheum officianl on the proliferation of renal tubular cells in vitro Zheng Feng ,Li Leishi Jinling Hospital,Nanjing 210002
Rat renal tubules were isolated and grown in culture medium.The effects of rheum officinal (RO) on the.proliferation of renal tubular cells were investigated.In culture,The additon of the sera obtained from the rat fed with RO inhibited significantly the proliferation of renal tubular cells,and the effect become even more strong with the increase of the dosage or the long time of adminstration of RO to the rat.The ingredient RO——emodin added directly to culture medium supressed the proliferation of renal tubular cells in a dose dependent manner,as measured by the uptake of radiolabeled thymidine.This observation may help explain the beneficial effects of RO in the treatment of chronic renal failure.
, http://www.100md.com
Key words Rheum officinal Kidney tubules Cell culture
(Original article on page 343)
Experimental study of portal hemodynamic changes after hepatic artery embolization Luo Baoming,et al.Digestive Department,Memorial Hospital,Sun Yat-Sen University of Medical sciences.Guangzhou 510120
We studied portal hemodynamic changes byusing duplex Doppler in 10 pigs with hepatic artery embolization (HAM) and hepatic artery ligation (HAL) and in 5 with HAL only.Serum concentration of alkline phosphatase,ALT,direct and indirect bilirubin were determined simultaneously.Hepatic angiographyand hepatic wvacular cast were performed in the 6pigs,and pathological examination in others.Portal venous velocity (PVV) and protal blood flow (PBF) increased after HAE.ALP,ALT,direct indirect bilirubin slightly elevated (p>0.05) in the two groups after operation and most of them decreased subsequently within 7 days.Linear regression of PBF with ALP,ALT,direct and indirect bilirubin occurred in the experimental group.Liver necrosis was seen in all pigs of the experimental group and only one in the control group.Angiography and vascular cast showed that hepatic artery collaterals occurred at the protal hilus in the two groups and were more rich in the control group.Extrahepatic and intrahepatic arterial collaterals were seen from 1 to 3 weeks after HAE.The estimation of PVV and PBF by duplex Doppler may reflect the development of collaterals in directly and is helpful in assessing the effect of HAE.
, http://www.100md.com
Key words Hepatic artery Animals,experimental Hemodynamics
(Original article on page 346)
Eeperimental observation of hing portal venous resistance in dogs Wang Weimin,et al.Department of General Srugery,First Teaching Hopital,Beijing Medical University,Beijing 100034
To study the relationship between portal resistance and formation of protal hypertension,we designed a high portal systemic resistance model by injecting bletilla hyacinthina intl the intrahepatic protal venulae through the portal vein in 9 dogs.The protal pressure was measured and the protal hemodynamics studied by ultrasonic Doppler before hemodynamics studied by ultrasonic Doppler before operation,and the data were collected with re-laparotomy after 10,20days respecively.Liver function,portal collateral circulation and liver histology were also observed.Our results suggest that the portal high resistance could cause portal hypertension.The elevation of FPP was maintained and portal blood flow was increased constantly in different degrees.The veins of the abdominal wass nad esophagus were dialted slightly.Histological study of the liver showed fibrotic hyperplasia in the portal area.Liver function was not merkedly changed except elevation of ALP.
, http://www.100md.com
It seems that the combination of portal resistance and portal hyperdynamics are the necessary pathogenic factor of formation of portal hypertension.
Key words Hypertension portal Portal system Disease models,animal Liver cirrohsis
(Original article on page 349)
Pathogenesis of skin pathologic changes and ulceration Qian Husheng,et al.Department of Vascular Srugery,Renij Holpital,Shanghai Second Medical University,Shanghai 200001
, http://www.100md.com
Twenty-six patients with venous insufficiency and 15 normal controls were studied by local skin pathological and ultrastructure changes.Transcutaneous oxygen tension (Tcpo2) and tPA,PAI of venous blood were measured.Biopsies from the ulcerating and liposclerosis area showed pericapillary fibrins.The patients with severe venous disease resulting in liposclerosis or ulceration the gaiter area had Tcpo2 levels lower than the controls and patients with venous insufficiency but no skin changes.The more serious the skin changes in patients with venous insufficiency,the lower the fibrinolysis activity.We conclude that extravascular deposition of fibrins in patients with venous disease which block the diffusion and exchanges of oxygen between the blood and tissue is an important factor in the development of venous ulcerations.The dectease of fibrinolysis activity led to the decrease of removing pericapillary fibrins edposition,combined with extravascular fibrin caused venous ulceration.
, 百拇医药
Key words Varicose ulcer Fibrosis Skin pigmentation
(Original article on page 352)
Clinical significance of TSH measurement by immunouadiometric assay and TRH test Luo Guochun,et al.Department of Endocrinology,Chinses PLA General Hospital,Beijing 100853
The levels of serun TSH were measured by a sensitive immunoradiometric assay (IRMA) method and the responses of TSH to TRH stimulation were observed in three groups of hyperthyroidism,primary hypothyroidism and secondary hyperthyroidism,primary hypothyroidism and secondary hypothyroidism.The levels of serum TSH were found to be undetected in 98% (1/51) of the patients with hyperthyroidism,very high in 100% (35/35) of the patients with primary hypothyroidism and normal in 91% (30/33) of the patients with secondary hypothyroidism.TRH test showed no responses of TSH in all patients with hyperhtyroisdism,high responses in all patients with primary hypothyroidsim and blunt responses in 69% (11/16) of patients with secondaryhypothyroidsim.The results indicat that the measurement of serum TSH by IRMA is a sensitive index in the diagnosis of hyperthyroidism and primary hypothyroidism,but can not be differentiated the secondary hypothyroidism from normal condistons.TRh test may be helpful in the differential diagnosis between secondary hypothyroidism and normal conditions.
, 百拇医药
Key words Thyrotropin Immunoradiometric assay Thyrotropin releasing hormone Hyperthyroisdism Hypothyroidism
(Original article on page 355)
Six elements change during development of mormal fetus vertebrae Zheng Bin,et al.Department of Pediatric Orthopedics,Second Affiliated Hospital,China Medical University,Shenyang 110003
Six elements:calcium,iron,zinc,copper,manganese and magnesium were detected in the central epiphyses of first lumber vertabrae in 60 normal aborted fetrs,aging from 9 to 32 weeks,by method of AAS.Iron,copper,manganese and zinc reached to their high levels during 9～20 weeks,which were related tothe formation of cartilage vertabrae.Calcium and magnesium,showed a significant relationship,and then gradually got to their high value after 16 weeks after ossification and remained at a constant level during later period.The data indicated that different elements should be given in different stage of fetus development.
Key words Fetal organ maturity Lumbar vertebrae Trace clements
(Original article on page 358), http://www.100md.com
单位：
关键词：
中华医学杂志930646 Hepatitis B X antigen binding to p53 protein in the pathogenesis of primary hepatocellular carcinoma Zhu Minghua,et al.Department of Pathology,Fourth Military Medical University,Xi'an 710032
Much evidences have showed a close relation between HBV infection and the development of primary hepatocellular carcinoma (PHC).Molecular genetic study showed loss of heterozygosity in PHC and that point mutation of p53 exon 7 at codon 249 was important to PHC.But these changes of PHC associated with aflatoxin B1 and HBV were not apparent.Thirteen cases of PHC were examined to determine whether there were any telationship between HBxAg and p53 protein.HBV-DNA was integrated to PHC tissue in 9 of the 13 cases by southern blot;HBsAg,HBxAg or HBcAg was found in 8 by immunohistochemical staining.None had the point mutation of p53 exon 7 at codon 229,247 and 249 by PCR and restriction enzymes.Immunoprecipitation analyses were carried out to test whether anti-X antiobody was capable of precipitating p53 or anti-p53 could immunoprecipitate HBxAg polypeptides from HBV infected tissue.Specific band was detected at 53000 in 7 of the 13 cases as precipitating with anti-HBxAg and western blotting using anti-p53,and specific band was detected at 13000,17000 or 28000 in 6 of the 13 cases as precipitating with anti-p53 and western blotting using anti-HBxAg.These results revealed that the binding of HBxAg to p53 may be analogous to that observed in tumor antigen encoded from selected papovaviruses,and adenoviruses,and may be a crucial step in the development of PHC among most HBV infected patients.
, http://www.100md.com
Key words Genes,p53 Hepatilis B virus Genes,regulator hepatoma
(Original article on page 325)
Distribution of HBV in testicle tissue of patients with hepatitis B infection Lang Zhenwei et al.Beijing Youan Hospital,Beijing 100054
To study distribution of HBV in the testicle tissue,we used polyclonal antibody and ABC method in detecting autopsy testicle in 34 cases.The positive rate of HBsAg and HBcAg was 52.9% and 11.8% respectively.The positive cells of spermatogonium,primary spermatocytes,spermatid and sertoli cells were single or grouped in clusters form in the semimiferous tubule.Of the 6 cases studied with in situ hybridization,2 han HBV DNA in spematogenic cells in individual nucleus.The findings support morphologically the transmission of HBV via sexual contact.
, 百拇医药
Key words Hepatitis B virus Immun-oenzyme technics Testis
(Original article on page 329)
Molecular cloning and sequencing of HCV gene from human blood Tan Deming,et al.Department of Infectious Diseases,First Affiliated Hospital,Hunan Medical University,Changsha 410008
To clone and characterize hepatitis C virus strain in China,we extracted RNA from the pooled plasma of persons with HCV infection in Hunan,China.HCV gene was amplified and cloned by RT-PCR and gene recombinant techniques.A total of 2 307 nucleotides of the complementary DNA clones of HCV(HCV-Hun),including the clone of 5' noncoding region (311bp),core region (340bp),envelope region (397bp),NSI region (634bp),NS3 region (245bp),and NS5 region (380bp),NS3 region (245bp),and NS5 region (380bp) were isolated and identified.The homologies in nucleotide sequuence between HCV-Hun and HCV-US or HCV-J were 98.1% and 98.7% respectively in 5' noncoding region;92.6% and 97.4% in core region;74.3% and 88.7% in envelope region;83.5% and 91.0% in NS1 region;82.9% and 94.7% in NS3 region;and 74.8% and 90.1% in NS5 region.The similar results were seen in deduced amino acid sequence homologies between above HCV stains.These findinges indicate that HCV-Hun were more mimic to HCV-J than HCV-US,and there were considerable heterogeneity in hepatitis C virus genomes isolated from different areas of the world.
, http://www.100md.com
Key word Hepatitis C virus Recombination,Genetic Cloning,Molecular Immunoenzyme technics
(Original article on page 332)
Hereditable characteristics of attenuated live hepatitis A virus H2 strain Chai Shoaai,et al.Virus Research Institute,Zhejiang Academy of Medical Sciences,Huangzhou 310013
The toxicity of attenuated hepatitis A virus H2 strain was not increased in testing KMB 17 cells of 5 consective generations of monkeys at 36℃.After injectio of attenuated live hepatitis A virus H2 strain in humans,no abnormality was noted.Serum hepatitis A antigen was positive after 38 children aged 3～9 years were given oral 106.5 TCKD50,whereas it was 100% positive in the group of same age after percutaneous vaccination.Noninfection of oral administration is and important hereditable characteristic differentiation from wild strains.
, 百拇医药
Key words Hepatitis A virus Vaccine Hereditable characteristics
(Original article on page 335)
Mapping of the transforming gene by fluorescence in situ hybridization He Hong,et al.Tumor Research Institute,Hunan Medical University,Changsha 410078.
A transforming gene of human nasopharynageal carcinoma was cloned from Chinese nasopharyngeal carcinoma cell line CNE2.This gene was different from a number of known oncogenes as was reported previously.The NPC transforming gene was 16kb in size,and the Alu negative 2.8kb fragment was cloned into pSP70 plasmid.Linearized Tx pSP70 recombinant plasmid was nick translated in the presence of biotin-11-dUTP,incubated with an excess of sonicated human DNA,and used as a probe for fluorescence in situ hybridization.Signals of hybridization were detected by successive treatments with FITC-labeled avidin.57% of grains were located on chromosome 8;51.8% of them were mapped to the 8q23→8q24.1 region of chromosome 8's.The Tx gene,therefore,could be assigned to the band 8q23→8q24.1.
, 百拇医药
Key words Nucleic acid hybridization Chromosome mapping Nasopharyngeal neoplasms
(Original article on page 338)
Distribution of extracellular viral antigen of epidemic hemorrhagic fever in human autopsy cases Liu Yanfang, et al.Depqrtment of Pathology,Fourth Military Medical University.Xi'an 710032
Using improved immunohistochemical methods,repeated PAP and repeated PAP+ABC,we found in the extracellular spaces of autopsy cases of epidemic hemorrhagic fever large amount of viral antigens.Two forms of antigens were found:diffuse soluble antigen and inclusion body like granular.The former was mainly G2 protein and the latter was mainly Nproteon.They were found in serum,lymph,CSF,edema fluid,fluids in renal,semenal tubules,alveolar and bronchial spaces,peptic,intestinal,pancreatic and thyroidal secretions.In most patients,the diffuse antigens were accompanied by IgG and C1q.A large amount of antigens,were excreted from the patients.
, http://www.100md.com
Key words Hemorrhagic fever,epidemics Body fluids Antigens,viral
(Original article on page 341)
Effect of rheum officianl on the proliferation of renal tubular cells in vitro Zheng Feng ,Li Leishi Jinling Hospital,Nanjing 210002
Rat renal tubules were isolated and grown in culture medium.The effects of rheum officinal (RO) on the.proliferation of renal tubular cells were investigated.In culture,The additon of the sera obtained from the rat fed with RO inhibited significantly the proliferation of renal tubular cells,and the effect become even more strong with the increase of the dosage or the long time of adminstration of RO to the rat.The ingredient RO——emodin added directly to culture medium supressed the proliferation of renal tubular cells in a dose dependent manner,as measured by the uptake of radiolabeled thymidine.This observation may help explain the beneficial effects of RO in the treatment of chronic renal failure.
, http://www.100md.com
Key words Rheum officinal Kidney tubules Cell culture
(Original article on page 343)
Experimental study of portal hemodynamic changes after hepatic artery embolization Luo Baoming,et al.Digestive Department,Memorial Hospital,Sun Yat-Sen University of Medical sciences.Guangzhou 510120
We studied portal hemodynamic changes byusing duplex Doppler in 10 pigs with hepatic artery embolization (HAM) and hepatic artery ligation (HAL) and in 5 with HAL only.Serum concentration of alkline phosphatase,ALT,direct and indirect bilirubin were determined simultaneously.Hepatic angiographyand hepatic wvacular cast were performed in the 6pigs,and pathological examination in others.Portal venous velocity (PVV) and protal blood flow (PBF) increased after HAE.ALP,ALT,direct indirect bilirubin slightly elevated (p>0.05) in the two groups after operation and most of them decreased subsequently within 7 days.Linear regression of PBF with ALP,ALT,direct and indirect bilirubin occurred in the experimental group.Liver necrosis was seen in all pigs of the experimental group and only one in the control group.Angiography and vascular cast showed that hepatic artery collaterals occurred at the protal hilus in the two groups and were more rich in the control group.Extrahepatic and intrahepatic arterial collaterals were seen from 1 to 3 weeks after HAE.The estimation of PVV and PBF by duplex Doppler may reflect the development of collaterals in directly and is helpful in assessing the effect of HAE.
, http://www.100md.com
Key words Hepatic artery Animals,experimental Hemodynamics
(Original article on page 346)
Eeperimental observation of hing portal venous resistance in dogs Wang Weimin,et al.Department of General Srugery,First Teaching Hopital,Beijing Medical University,Beijing 100034
To study the relationship between portal resistance and formation of protal hypertension,we designed a high portal systemic resistance model by injecting bletilla hyacinthina intl the intrahepatic protal venulae through the portal vein in 9 dogs.The protal pressure was measured and the protal hemodynamics studied by ultrasonic Doppler before hemodynamics studied by ultrasonic Doppler before operation,and the data were collected with re-laparotomy after 10,20days respecively.Liver function,portal collateral circulation and liver histology were also observed.Our results suggest that the portal high resistance could cause portal hypertension.The elevation of FPP was maintained and portal blood flow was increased constantly in different degrees.The veins of the abdominal wass nad esophagus were dialted slightly.Histological study of the liver showed fibrotic hyperplasia in the portal area.Liver function was not merkedly changed except elevation of ALP.
, http://www.100md.com
It seems that the combination of portal resistance and portal hyperdynamics are the necessary pathogenic factor of formation of portal hypertension.
Key words Hypertension portal Portal system Disease models,animal Liver cirrohsis
(Original article on page 349)
Pathogenesis of skin pathologic changes and ulceration Qian Husheng,et al.Department of Vascular Srugery,Renij Holpital,Shanghai Second Medical University,Shanghai 200001
, http://www.100md.com
Twenty-six patients with venous insufficiency and 15 normal controls were studied by local skin pathological and ultrastructure changes.Transcutaneous oxygen tension (Tcpo2) and tPA,PAI of venous blood were measured.Biopsies from the ulcerating and liposclerosis area showed pericapillary fibrins.The patients with severe venous disease resulting in liposclerosis or ulceration the gaiter area had Tcpo2 levels lower than the controls and patients with venous insufficiency but no skin changes.The more serious the skin changes in patients with venous insufficiency,the lower the fibrinolysis activity.We conclude that extravascular deposition of fibrins in patients with venous disease which block the diffusion and exchanges of oxygen between the blood and tissue is an important factor in the development of venous ulcerations.The dectease of fibrinolysis activity led to the decrease of removing pericapillary fibrins edposition,combined with extravascular fibrin caused venous ulceration.
, 百拇医药
Key words Varicose ulcer Fibrosis Skin pigmentation
(Original article on page 352)
Clinical significance of TSH measurement by immunouadiometric assay and TRH test Luo Guochun,et al.Department of Endocrinology,Chinses PLA General Hospital,Beijing 100853
The levels of serun TSH were measured by a sensitive immunoradiometric assay (IRMA) method and the responses of TSH to TRH stimulation were observed in three groups of hyperthyroidism,primary hypothyroidism and secondary hyperthyroidism,primary hypothyroidism and secondary hypothyroidism.The levels of serum TSH were found to be undetected in 98% (1/51) of the patients with hyperthyroidism,very high in 100% (35/35) of the patients with primary hypothyroidism and normal in 91% (30/33) of the patients with secondary hypothyroidism.TRH test showed no responses of TSH in all patients with hyperhtyroisdism,high responses in all patients with primary hypothyroidsim and blunt responses in 69% (11/16) of patients with secondaryhypothyroidsim.The results indicat that the measurement of serum TSH by IRMA is a sensitive index in the diagnosis of hyperthyroidism and primary hypothyroidism,but can not be differentiated the secondary hypothyroidism from normal condistons.TRh test may be helpful in the differential diagnosis between secondary hypothyroidism and normal conditions.
, 百拇医药
Key words Thyrotropin Immunoradiometric assay Thyrotropin releasing hormone Hyperthyroisdism Hypothyroidism
(Original article on page 355)
Six elements change during development of mormal fetus vertebrae Zheng Bin,et al.Department of Pediatric Orthopedics,Second Affiliated Hospital,China Medical University,Shenyang 110003
Six elements:calcium,iron,zinc,copper,manganese and magnesium were detected in the central epiphyses of first lumber vertabrae in 60 normal aborted fetrs,aging from 9 to 32 weeks,by method of AAS.Iron,copper,manganese and zinc reached to their high levels during 9～20 weeks,which were related tothe formation of cartilage vertabrae.Calcium and magnesium,showed a significant relationship,and then gradually got to their high value after 16 weeks after ossification and remained at a constant level during later period.The data indicated that different elements should be given in different stage of fetus development.
Key words Fetal organ maturity Lumbar vertebrae Trace clements
(Original article on page 358), http://www.100md.com