卵巢分化型上皮肿瘤端粒酶Htrt基因、p53、C-erb-B2蛋白检测与比较
作者：卫建平 张波 沈容 李圣爱 侯琳
单位：卫建平(山西医科大学第二附属医院病理科 030001)；张波 沈容 李圣爱 侯琳(北京医科大学病理学系)
关键词：hTRT；C-erb-B2；p53；原位杂交；卵巢肿瘤
诊断病理学杂志000112摘 要：目的 探讨检测hTRT基因表达在卵巢分化型上皮源肿瘤中的价值。方法 用原位杂交检测75例卵巢良性(43例)、低度恶性(8例)、恶性上皮源肿瘤(24例)hTRT基因表达，同时用免疫组化检测p53，G-erb-B2蛋白表达。结果 75例肿瘤中，hTRT基因：良性肿瘤12%(5/43)；低度恶性肿瘤38%(3/8)；恶性肿瘤96%(23/24)，三者间存在着显著性差异(P0.05)。hTRT与p53，C-erb-B2的表达均无相关性(P>0.05)。结论 端粒酶hTRT基因在恶性卵巢分化型上皮源肿瘤中有显著性改变，其基因的表达较p53，C-erb-B2蛋白改变更为普遍，提示检测hTRT表达可作为恶性卵巢上皮性肿瘤诊断的指标。
, http://www.100md.com
分类号： R730.261 文献标识码：A
文章编号：1007-8096(2000)01-0033-03
Expression of telomerase hTRT in differentiated ovarian epithelial tumors—comparison with alteration of p53 and c-erb-B2
Wei Jianping,Zhang Bo,Shen Rong
(Department of Pathology,The Second/Affiliated Hospital, Shanxi Medical University, Taiyuan 030001)
Abstract：Objective To evaluate detection of telomerase hTRT expression in ovarian epithelial tumor. Methods Total 75 cases of ovarian epithelial tumors,including 43 cases of benign tumors,8 borderline and 24 malignant tumors,were analyzed with in situ hybridization and immunohistochemistry. Results Expression of hTRT was positively detected in 12% (5/43) be-nign, 38% (3/8) borderline and 96% (23/24) malignant tumors.A significant difference among the three groups was found (P0.05). Conclusions Expression of telomerase hTRT was significantly correlated with the malignancy of ovarian epithelial tumors, and more frequent positive than p53 and c-erb-B2.It is suggested that hTRT expression may be a good marker in diagnosis of malignant ovarian epithelial tumors.
, 百拇医药
Keywords：hTRT p53 c-erb-B2 In situ hybridization Ovarian tumor
参考文献：
[1]Meyerson M,Counter CM.hEST2 the putative human telomerase catalytic subunit gene is up-regulated during immortalization.Cell, 1997,90:785-95
[2]应建明，金燕燕，王衡，等.hTRT催化功能区基因克隆及其在肿瘤中的表达.中华病理学杂志，1999,28:85-88
[3]Counter CM,Hirte HW, Bacchetti S,et al. Telomerase activity in human overian carcinoma. Proc Natl Acad Sci USA,1994,91:2900-4
, 百拇医药
[4]Kyo S, Kanaya T, Ishikawa H,et al. Telomerase activity in gynecological tumors. Clin Cancer Res,1996,2:2023
[5]Kim NW,Piatyszek MA, Prowse KR,et al.Specific association of human telemerase activity with immortal cells and cancer.Science,1994,266:2011-5
[6]Shay JW, Bacchetti S. A survey d telemerase activay in human cancer.Eur J cancer,1997,33:787-91
[7]Kyo S, Kanaya T,Takakura M, et al.Expression of human telemerase sub-units in ovarian malignant,borderline and benign tumors.Int J Cancer,1999,80:804-9
, 百拇医药
[8]Kyo S,Kanaya T,Takakura M,et al. Human telomerase reverse transcrip-tase as a critical determinant of telemerase activity in normal and malig-nant endometrial fissues.Int J Cancer,1999,80:60-3
[9]Yokoyama Y,Takahashi Y,Shinohara A,et al. Telomerase activity in the fe-male reproductive tract and neoplasms. Gynecol Oncol,1998,68:145-9
[10]Wan M, Li WZ, Duggan BD,et al.Telomerase activity in benign and ma-lignant epithelial ovarian tumors.J Natl Cancer Inst,1997,89:437-41
[11]Oishi T,Kisawa J,Minagawa Y,et al.Alteration of telomerase activity associated with development and extension of epithelial ovarian cancer.Obstet Gynecol,1998,91:568-71
收稿日期：1999-04-08
修稿日期：1999-06-15, http://www.100md.com
单位：卫建平(山西医科大学第二附属医院病理科 030001)；张波 沈容 李圣爱 侯琳(北京医科大学病理学系)
关键词：hTRT；C-erb-B2；p53；原位杂交；卵巢肿瘤
诊断病理学杂志000112摘 要：目的 探讨检测hTRT基因表达在卵巢分化型上皮源肿瘤中的价值。方法 用原位杂交检测75例卵巢良性(43例)、低度恶性(8例)、恶性上皮源肿瘤(24例)hTRT基因表达，同时用免疫组化检测p53，G-erb-B2蛋白表达。结果 75例肿瘤中，hTRT基因：良性肿瘤12%(5/43)；低度恶性肿瘤38%(3/8)；恶性肿瘤96%(23/24)，三者间存在着显著性差异(P0.05)。hTRT与p53，C-erb-B2的表达均无相关性(P>0.05)。结论 端粒酶hTRT基因在恶性卵巢分化型上皮源肿瘤中有显著性改变，其基因的表达较p53，C-erb-B2蛋白改变更为普遍，提示检测hTRT表达可作为恶性卵巢上皮性肿瘤诊断的指标。
, http://www.100md.com
分类号： R730.261 文献标识码：A
文章编号：1007-8096(2000)01-0033-03
Expression of telomerase hTRT in differentiated ovarian epithelial tumors—comparison with alteration of p53 and c-erb-B2
Wei Jianping,Zhang Bo,Shen Rong
(Department of Pathology,The Second/Affiliated Hospital, Shanxi Medical University, Taiyuan 030001)
Abstract：Objective To evaluate detection of telomerase hTRT expression in ovarian epithelial tumor. Methods Total 75 cases of ovarian epithelial tumors,including 43 cases of benign tumors,8 borderline and 24 malignant tumors,were analyzed with in situ hybridization and immunohistochemistry. Results Expression of hTRT was positively detected in 12% (5/43) be-nign, 38% (3/8) borderline and 96% (23/24) malignant tumors.A significant difference among the three groups was found (P0.05). Conclusions Expression of telomerase hTRT was significantly correlated with the malignancy of ovarian epithelial tumors, and more frequent positive than p53 and c-erb-B2.It is suggested that hTRT expression may be a good marker in diagnosis of malignant ovarian epithelial tumors.
, 百拇医药
Keywords：hTRT p53 c-erb-B2 In situ hybridization Ovarian tumor
参考文献：
[1]Meyerson M,Counter CM.hEST2 the putative human telomerase catalytic subunit gene is up-regulated during immortalization.Cell, 1997,90:785-95
[2]应建明，金燕燕，王衡，等.hTRT催化功能区基因克隆及其在肿瘤中的表达.中华病理学杂志，1999,28:85-88
[3]Counter CM,Hirte HW, Bacchetti S,et al. Telomerase activity in human overian carcinoma. Proc Natl Acad Sci USA,1994,91:2900-4
, 百拇医药
[4]Kyo S, Kanaya T, Ishikawa H,et al. Telomerase activity in gynecological tumors. Clin Cancer Res,1996,2:2023
[5]Kim NW,Piatyszek MA, Prowse KR,et al.Specific association of human telemerase activity with immortal cells and cancer.Science,1994,266:2011-5
[6]Shay JW, Bacchetti S. A survey d telemerase activay in human cancer.Eur J cancer,1997,33:787-91
[7]Kyo S, Kanaya T,Takakura M, et al.Expression of human telemerase sub-units in ovarian malignant,borderline and benign tumors.Int J Cancer,1999,80:804-9
, 百拇医药
[8]Kyo S,Kanaya T,Takakura M,et al. Human telomerase reverse transcrip-tase as a critical determinant of telemerase activity in normal and malig-nant endometrial fissues.Int J Cancer,1999,80:60-3
[9]Yokoyama Y,Takahashi Y,Shinohara A,et al. Telomerase activity in the fe-male reproductive tract and neoplasms. Gynecol Oncol,1998,68:145-9
[10]Wan M, Li WZ, Duggan BD,et al.Telomerase activity in benign and ma-lignant epithelial ovarian tumors.J Natl Cancer Inst,1997,89:437-41
[11]Oishi T,Kisawa J,Minagawa Y,et al.Alteration of telomerase activity associated with development and extension of epithelial ovarian cancer.Obstet Gynecol,1998,91:568-71
收稿日期：1999-04-08
修稿日期：1999-06-15, http://www.100md.com