2型糖尿病患者载脂蛋白B基因型频率分布的初步探讨
作者：郑景晨 俞 康 沈飞霞
单位：325000 温州医学院附属第一医院内医科所
关键词：2型糖尿病载脂蛋白B基因聚合酶链反应-限制性片段长度多态性高脂血症
浙江临床医学000104摘 要：目的：研究载脂蛋白B(ApoB)基因多态性在本地区2型糖尿病患者中的分布及其与血脂的关系。方法：运用聚合酶链反应一限制性片段长度多态性(PCR—RFLP)方法检测41例2型糖尿病患者及60例正常对照者的ApoB基因型。结果：两组X+和E-等位基因频率分别为0.085，0.017和0.11，0.033(p<0.01)。糖尿病组血脂总胆固醇(CH)、甘油三脂(TG)、低密度脂蛋白胆固醇(LDL-ch)水平均高于对照组(p<0.01)；而高密度脂蛋白胆固醇(HDL-ch)水平低于对照组(p<0.05)。结论：ApoB的X+和E-等位基因可能是2型糖尿病和高脂血症的一个危险因子。
, 百拇医药
Preliminary Exploration on Relationship between the Polymorphism of Apolipoprotein B(ApoB)Gene and Type 2 Diabetes Mellitus(D M)
Zheng Jingchen
Abstract：Objective: To investigate the relationship getween the poiymorphism of apolipaproteinB (ApoB) gene and type 2 diabetes mellitus (DM) and its serum lipid changes. Methods: The ApoB genetype in 41 type 2 diabetes mellitus and 60 normal control subjects were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The frequencies of X+ and E- alleles in these two groups were respectively 0.085,0. 017 and 0.11, 0. 033 (P<0.01) . The levels of senan cholesterol (CH), triglyceride (TG) and low density lipoprotein-cholesterol (LDL-ch) in type 2 DM patients were significantly higher than control subjects (p<0.01), and the levels of high density lopoprotein-cholesterol (HDL-ch) were lower than control (P<0.05). Conclusion: The results suggest that X+ and E- alleles probably serve as risk factors for type 2 DM and hyperlipidemias.
, 百拇医药
Keywords：Type 2 diabetes mellitus Apolipoprotein B gene PCR-RFLP Hyperlipidemia
参考文献：
[1]叶平，王士雯，陈保生，等.载脂蛋白B基因多态性与冠心病关系的研究.中华心血管杂志，1994，22：119.
[2]俞 康，吴建波，沈志坚，等.再生障碍性贫血患者的载脂蛋白B，E基因多态性分析.中华血液学杂志，1998，19：538.
[3]Houlston RS，Snowden C，Laker MF，et al.Variation in the apolipoprotein B gene and development of type 2 diabetes mellims.DisMarkers，1991，9：87.
, http://www.100md.com
[4]郭立新，杨香玖，张颖.载脂蛋白B基因多态性与Ⅱ型糖尿病并冠心病关系的研究.中华内分泌代谢杂志，1997，13：137.
[5]Hansen TA，Nordestgarrd BG，Gerdes LU，et al.Variation of apolipoportein B gene is associated with myocardial infarction and lipoprotein levels in Danes.Atherosclerosis，1991，89：69.
[6]Humphries SE.DNA polymorphisms of the apolipoprotein genes their use in the investigation of the genetic component of hypefiipidaemia and atherosclerosis.Atherosclerosis，1988，72：89., 百拇医药
单位：325000 温州医学院附属第一医院内医科所
关键词：2型糖尿病载脂蛋白B基因聚合酶链反应-限制性片段长度多态性高脂血症
浙江临床医学000104摘 要：目的：研究载脂蛋白B(ApoB)基因多态性在本地区2型糖尿病患者中的分布及其与血脂的关系。方法：运用聚合酶链反应一限制性片段长度多态性(PCR—RFLP)方法检测41例2型糖尿病患者及60例正常对照者的ApoB基因型。结果：两组X+和E-等位基因频率分别为0.085，0.017和0.11，0.033(p<0.01)。糖尿病组血脂总胆固醇(CH)、甘油三脂(TG)、低密度脂蛋白胆固醇(LDL-ch)水平均高于对照组(p<0.01)；而高密度脂蛋白胆固醇(HDL-ch)水平低于对照组(p<0.05)。结论：ApoB的X+和E-等位基因可能是2型糖尿病和高脂血症的一个危险因子。
, 百拇医药
Preliminary Exploration on Relationship between the Polymorphism of Apolipoprotein B(ApoB)Gene and Type 2 Diabetes Mellitus(D M)
Zheng Jingchen
Abstract：Objective: To investigate the relationship getween the poiymorphism of apolipaproteinB (ApoB) gene and type 2 diabetes mellitus (DM) and its serum lipid changes. Methods: The ApoB genetype in 41 type 2 diabetes mellitus and 60 normal control subjects were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The frequencies of X+ and E- alleles in these two groups were respectively 0.085,0. 017 and 0.11, 0. 033 (P<0.01) . The levels of senan cholesterol (CH), triglyceride (TG) and low density lipoprotein-cholesterol (LDL-ch) in type 2 DM patients were significantly higher than control subjects (p<0.01), and the levels of high density lopoprotein-cholesterol (HDL-ch) were lower than control (P<0.05). Conclusion: The results suggest that X+ and E- alleles probably serve as risk factors for type 2 DM and hyperlipidemias.
, 百拇医药
Keywords：Type 2 diabetes mellitus Apolipoprotein B gene PCR-RFLP Hyperlipidemia
参考文献：
[1]叶平，王士雯，陈保生，等.载脂蛋白B基因多态性与冠心病关系的研究.中华心血管杂志，1994，22：119.
[2]俞 康，吴建波，沈志坚，等.再生障碍性贫血患者的载脂蛋白B，E基因多态性分析.中华血液学杂志，1998，19：538.
[3]Houlston RS，Snowden C，Laker MF，et al.Variation in the apolipoprotein B gene and development of type 2 diabetes mellims.DisMarkers，1991，9：87.
, http://www.100md.com
[4]郭立新，杨香玖，张颖.载脂蛋白B基因多态性与Ⅱ型糖尿病并冠心病关系的研究.中华内分泌代谢杂志，1997，13：137.
[5]Hansen TA，Nordestgarrd BG，Gerdes LU，et al.Variation of apolipoportein B gene is associated with myocardial infarction and lipoprotein levels in Danes.Atherosclerosis，1991，89：69.
[6]Humphries SE.DNA polymorphisms of the apolipoprotein genes their use in the investigation of the genetic component of hypefiipidaemia and atherosclerosis.Atherosclerosis，1988，72：89., 百拇医药