HGV／GBV-C与HCV混合感染者肝组织中相关病毒表达的免疫组化研究
作者：聂青和 胡大荣 谢青 李梦东 李玲 朱永红
单位：聂青和(第四军医大学唐都医院传染病科 西安 710038)；胡大荣(解放军肝病研究所；谢青(第三军医大学新桥医院病理科)；李梦东 李玲 朱永红(第三军医大学西南医院全军传染病专科中心)
关键词：庚型肝炎病毒；GB病毒C型；丙型肝炎病毒；混合感染；抗原；免疫组织化学
诊断病理学杂志000216摘 要：目的了解HGV/GBV-C与HCV混合感染者肝组织中HGV/GBV-C相关抗原的分布状况，探讨HGV/GBVC对肝脏的损害机制。方法以抗HGV/GBV-CNS5单克隆抗体或抗HCVNS3单克隆抗体为试剂，采用免疫组织化学方法检测肝炎病人肝组织中HGV/GBV-C、HCV相关抗原表达。结果56例肝炎病人肝组织中HGV/GBV-C相关抗原表达阳性率为26.79%(15/56)；HCVNS3抗原表达阳性率为39.29%(22/56)。HGV/GBV-CNS5抗原表达阳性信号主要位于肝细胞胞浆中，染色阳性细胞周围可见淋巴细胞浸润。结论肝细胞中存在HGV/GBV-C相关抗原表达，其编码产物可能作为一种靶抗原，诱发免疫病理反应，免疫损伤可能是其发病机制之一。
, http://www.100md.com
分类号：R512.6 文献标识码：A
文章编号：1007-8096(2000)02-0121-03
Immunohistochemical Studies of HGV/GBV-C and HCV-related Antigens in the Liver Tissues of the Patients Co-infected with Hepatitis C and G Viruses
Nie Qinghe ,Hu Darong ,Xie Qing
(Tangdu Hospital，Fourth Military medical University，Xi’an 710038)
Abstract：Objective To investigate the distribution of hepatitis G virus(HGV)/GB virus C (GBV-C)related antigens in the liver tissues of the patients co-infected with hepatitis C and G viruses and to discuss underlying mechanism of liver damages induced by HGV/GBV-C. Methods HGV/GBV-C or HCV-related antigens were detected with immunohistochemical methods，by using NS-5 and NS-3 monoclonal antibodies，in paraffin embedded liver tissues. Results Positive rate of HGV/GBV-C-related antigens was 26.79%(15/56)，and HCV NS3 39.29%(22/56)in 56 cases of the hepatitis.Expression of HGV/GBV-C NS5 antigen mostly limited in the cytoplasm of liver cells，which was surrounded by lymphocytic infiltration.Conclusion HGV/GBV-C related antigens are expressed in the liver cells.HGV/GBV-C encoded product may be a target antigen，which induces immune reactions.Tne immune damage could be one of the pathogeneses of the hepatitis.
, 百拇医药
Keywords：HGV GBV-C HCV Mixed infection Antigen Immunohistochemistry
参考文献：
[1]Miyakawa Y，Mayumi M.Hepatitis G virus-A true hepatitis virus or an accidental tourist?N Engl J Med,1997,336:795-796
[2]Bralet MP，Roudot-Thoraral F，Pawlotsky JM，et al.Histopathologic impact GB virus C infection on chronic hepatitis C.Gastroenterology，1997,112：188-192
[3]李 玲，聂青和，陈国致，等.庚型肝炎病毒抗体在不同人群中的检测及意义.实用肝脏病杂志，1998,3:9-11
, http://www.100md.com
[4]病毒性肝炎防治方案(试行).中华传染病杂志,1995,13:241-247
[5]Desmet VJ，Gerber M，Hoofnagle JH，et al.Classification of chronic hepatitis：diagnosis，grading and staging.Hepatology,1994,19:1513-1520
[6]聂青和.己型、庚型及其它新型肝炎病毒研究概况.国外医学流行病学传染病学分册,1997,24:19-23
[7]Pessoa MG，Terrault NA，Detmer J,et al.Quantitation of hepatitis G and C viruses in the liver：Evidence that hepatits G virus in not hepatotropic.Hepatology，1998,27:877-880
, 百拇医药
[8]聂青和，谢 青，胡大荣，等.庚型肝炎病毒感染者肝组织中庚型肝炎病毒相关抗原的表达.第三军医大学学报,1997,19:394
[9]Hiramatsu N，Hayashi N，Haruna Y，et al.Immunohistochemical detection of hepatitis C virus infected hepatocytes in chronic liver disease with monoclonal antibodies to core，envelope and NS3 regions of the hepatitis C virus genome.Hepatology，1992,16:306-310
[10]Tsutsumi M，Urashima S，Takada A，et al.Detection of antigens related to hepatitis C virus RNA encoding the NS5 region in the livers of patients with chronic type C hepatitis.Hepatology，1994,19:265-272
收稿日期：1999-12-22
修稿日期：2000-01-30, 百拇医药
单位：聂青和(第四军医大学唐都医院传染病科 西安 710038)；胡大荣(解放军肝病研究所；谢青(第三军医大学新桥医院病理科)；李梦东 李玲 朱永红(第三军医大学西南医院全军传染病专科中心)
关键词：庚型肝炎病毒；GB病毒C型；丙型肝炎病毒；混合感染；抗原；免疫组织化学
诊断病理学杂志000216摘 要：目的了解HGV/GBV-C与HCV混合感染者肝组织中HGV/GBV-C相关抗原的分布状况，探讨HGV/GBVC对肝脏的损害机制。方法以抗HGV/GBV-CNS5单克隆抗体或抗HCVNS3单克隆抗体为试剂，采用免疫组织化学方法检测肝炎病人肝组织中HGV/GBV-C、HCV相关抗原表达。结果56例肝炎病人肝组织中HGV/GBV-C相关抗原表达阳性率为26.79%(15/56)；HCVNS3抗原表达阳性率为39.29%(22/56)。HGV/GBV-CNS5抗原表达阳性信号主要位于肝细胞胞浆中，染色阳性细胞周围可见淋巴细胞浸润。结论肝细胞中存在HGV/GBV-C相关抗原表达，其编码产物可能作为一种靶抗原，诱发免疫病理反应，免疫损伤可能是其发病机制之一。
, http://www.100md.com
分类号：R512.6 文献标识码：A
文章编号：1007-8096(2000)02-0121-03
Immunohistochemical Studies of HGV/GBV-C and HCV-related Antigens in the Liver Tissues of the Patients Co-infected with Hepatitis C and G Viruses
Nie Qinghe ,Hu Darong ,Xie Qing
(Tangdu Hospital，Fourth Military medical University，Xi’an 710038)
Abstract：Objective To investigate the distribution of hepatitis G virus(HGV)/GB virus C (GBV-C)related antigens in the liver tissues of the patients co-infected with hepatitis C and G viruses and to discuss underlying mechanism of liver damages induced by HGV/GBV-C. Methods HGV/GBV-C or HCV-related antigens were detected with immunohistochemical methods，by using NS-5 and NS-3 monoclonal antibodies，in paraffin embedded liver tissues. Results Positive rate of HGV/GBV-C-related antigens was 26.79%(15/56)，and HCV NS3 39.29%(22/56)in 56 cases of the hepatitis.Expression of HGV/GBV-C NS5 antigen mostly limited in the cytoplasm of liver cells，which was surrounded by lymphocytic infiltration.Conclusion HGV/GBV-C related antigens are expressed in the liver cells.HGV/GBV-C encoded product may be a target antigen，which induces immune reactions.Tne immune damage could be one of the pathogeneses of the hepatitis.
, 百拇医药
Keywords：HGV GBV-C HCV Mixed infection Antigen Immunohistochemistry
参考文献：
[1]Miyakawa Y，Mayumi M.Hepatitis G virus-A true hepatitis virus or an accidental tourist?N Engl J Med,1997,336:795-796
[2]Bralet MP，Roudot-Thoraral F，Pawlotsky JM，et al.Histopathologic impact GB virus C infection on chronic hepatitis C.Gastroenterology，1997,112：188-192
[3]李 玲，聂青和，陈国致，等.庚型肝炎病毒抗体在不同人群中的检测及意义.实用肝脏病杂志，1998,3:9-11
, http://www.100md.com
[4]病毒性肝炎防治方案(试行).中华传染病杂志,1995,13:241-247
[5]Desmet VJ，Gerber M，Hoofnagle JH，et al.Classification of chronic hepatitis：diagnosis，grading and staging.Hepatology,1994,19:1513-1520
[6]聂青和.己型、庚型及其它新型肝炎病毒研究概况.国外医学流行病学传染病学分册,1997,24:19-23
[7]Pessoa MG，Terrault NA，Detmer J,et al.Quantitation of hepatitis G and C viruses in the liver：Evidence that hepatits G virus in not hepatotropic.Hepatology，1998,27:877-880
, 百拇医药
[8]聂青和，谢 青，胡大荣，等.庚型肝炎病毒感染者肝组织中庚型肝炎病毒相关抗原的表达.第三军医大学学报,1997,19:394
[9]Hiramatsu N，Hayashi N，Haruna Y，et al.Immunohistochemical detection of hepatitis C virus infected hepatocytes in chronic liver disease with monoclonal antibodies to core，envelope and NS3 regions of the hepatitis C virus genome.Hepatology，1992,16:306-310
[10]Tsutsumi M，Urashima S，Takada A，et al.Detection of antigens related to hepatitis C virus RNA encoding the NS5 region in the livers of patients with chronic type C hepatitis.Hepatology，1994,19:265-272
收稿日期：1999-12-22
修稿日期：2000-01-30, 百拇医药