宫颈癌组织中HPVl6.18E6蛋白的表达及其与p53蛋白表达的关系
作者：张伟 李艳红 刘节 苏勤 赵建业
单位：第四军医大学唐都医院病理科西安 710038
关键词：宫颈肿瘤；乳头状瘤病毒；基因；p53
诊断病理学杂志000214摘 要：目的观察人乳头状瘤病毒(HPV)16型和18型早期蛋白E6及p53蛋白在宫颈癌(CCa)中的表达，并分析二者的关系。方法采用LSAB免疫组化法，检测多种宫颈病变中HPVl6.18E6蛋白的表达及CCa中p53蛋白的表达。标本包括正常宫颈组织40例，宫颈湿疣44例，宫颈上皮肉瘤变(CIN)Ⅰ～Ⅱ级45例，CINⅢ级41例，CCa52例。结果上述宫颈病变组织中HPVl6、18E6总阳性率分别为:2.0%，7.1%，22.2%，43.9%和67.3%，各病变组阳性率均显著高于正常宫颈组(P<0.01)；54例CCa中p53蛋白阳性者6例(11.5%)，均为鳞癌，其中3例伴有HPVl6.18E6阳性。结论HPVl6.18型感染与CCa及其癌前病变的发生关系密切，HPVl6.18E6单克隆抗体可作为检测HPVl6.18感染及CCa早期诊断的标记之一。本研究显示p53基因突变与HPV感染无显著相关性。
, http://www.100md.com
分类号：R737.33 文献标识码：A
文章编号：1007-8096(2000)02-0116-03
Expression of HPV16 and l8E6 protein in Cervical Carcinomas and its Correlation with p53 Alteration
Zhang Wei ,Li Yanhong ,Liu Jie
(Department of Pathology，Tangdu Hospital，Fourth Military Medical University，Xi’an 710038)
Abstract：Objective To investigate the expression of human papilloma virus(HPV)16 and l8E6，and p53 protein in human cervical carcinoma(CCa)，and to evaluate relationship between them. Methods LSAB immunohistochemistry was used to detect HPV 16 and 18E6 and p53 proteins in 52 cases of CCa，4l cervical intraepithelial neoplasia(CIN)Ⅲ，45 CIN Ⅰ～Ⅱ，44 cervical condyloma and 40 normal cervixes as control. Results The positive rates of HPV 16 and 18E6 in the above five groups were 67.3%，43.9%，22.2%，7.1%and 2.0%respectively，with a significant difference between the cervical lesions and normal cervix(P<0.01).Moreover，6 of the 54 cases of CCa presented high level of p53 protein，of 3 cases showing positivities of HPVl6 and 18E6 proteins.Conclusion HPV 16 and 18 infections are closely related with cervical condyloma，CIN and CCa.HPVl6 and 18 antibodies could be good indicators for the viruses infection and one of markers for early diagnosis of CCa.This study did not indicate a direct relationship between p53 mutation and HPVl6 and l8 infections.
, http://www.100md.com
Keywords：Cervical tumors Papilloma virus Gene p53
参考文献：
[1]Howley pM. Role of the human papilomavirus in human cancer. Cancer Res,1991,51:5019-5022
[2]Stonbridge Euman. Tumor suppressor genes. Annul Rev Genet, 1990,24:615
[3]Harris CC. p53: At the Crossroads of Molecullar carcinogenesis and Risk Assessment. Science, 1993,262:1980-1981
, http://www.100md.com
[4]Munger K, Phelps WC, Bubb V. The E6 and E7 gene of human papillovirus type 16 together are necessary sufficient for transformation ofprimary human keratinocytes.Jvirol.1989,63:4417-21
[5]MirandaT，Paola M，John J，et al.HPV-18E6 mediated inhitition of p53 DNA binding activity is independent of E6 induced degradation.Oncogene.1995,10:261-268
[6]Mark S，Lechner,Laimonis A，Laimins.Inhibition of p53 DNA binding by human papillovirus E6 protein.Journal of virology.1994,68:4262-4273
[7]Smith ML,Bortnick RA,Sheikh MS,et al.Chromatin relaxation by overex pression of mutant p53，HPVl6-E6，or cyclin G transgenes.ExpCell Res.1998,242:235-243
收稿日期：1999-10-09, http://www.100md.com
单位：第四军医大学唐都医院病理科西安 710038
关键词：宫颈肿瘤；乳头状瘤病毒；基因；p53
诊断病理学杂志000214摘 要：目的观察人乳头状瘤病毒(HPV)16型和18型早期蛋白E6及p53蛋白在宫颈癌(CCa)中的表达，并分析二者的关系。方法采用LSAB免疫组化法，检测多种宫颈病变中HPVl6.18E6蛋白的表达及CCa中p53蛋白的表达。标本包括正常宫颈组织40例，宫颈湿疣44例，宫颈上皮肉瘤变(CIN)Ⅰ～Ⅱ级45例，CINⅢ级41例，CCa52例。结果上述宫颈病变组织中HPVl6、18E6总阳性率分别为:2.0%，7.1%，22.2%，43.9%和67.3%，各病变组阳性率均显著高于正常宫颈组(P<0.01)；54例CCa中p53蛋白阳性者6例(11.5%)，均为鳞癌，其中3例伴有HPVl6.18E6阳性。结论HPVl6.18型感染与CCa及其癌前病变的发生关系密切，HPVl6.18E6单克隆抗体可作为检测HPVl6.18感染及CCa早期诊断的标记之一。本研究显示p53基因突变与HPV感染无显著相关性。
, http://www.100md.com
分类号：R737.33 文献标识码：A
文章编号：1007-8096(2000)02-0116-03
Expression of HPV16 and l8E6 protein in Cervical Carcinomas and its Correlation with p53 Alteration
Zhang Wei ,Li Yanhong ,Liu Jie
(Department of Pathology，Tangdu Hospital，Fourth Military Medical University，Xi’an 710038)
Abstract：Objective To investigate the expression of human papilloma virus(HPV)16 and l8E6，and p53 protein in human cervical carcinoma(CCa)，and to evaluate relationship between them. Methods LSAB immunohistochemistry was used to detect HPV 16 and 18E6 and p53 proteins in 52 cases of CCa，4l cervical intraepithelial neoplasia(CIN)Ⅲ，45 CIN Ⅰ～Ⅱ，44 cervical condyloma and 40 normal cervixes as control. Results The positive rates of HPV 16 and 18E6 in the above five groups were 67.3%，43.9%，22.2%，7.1%and 2.0%respectively，with a significant difference between the cervical lesions and normal cervix(P<0.01).Moreover，6 of the 54 cases of CCa presented high level of p53 protein，of 3 cases showing positivities of HPVl6 and 18E6 proteins.Conclusion HPV 16 and 18 infections are closely related with cervical condyloma，CIN and CCa.HPVl6 and 18 antibodies could be good indicators for the viruses infection and one of markers for early diagnosis of CCa.This study did not indicate a direct relationship between p53 mutation and HPVl6 and l8 infections.
, http://www.100md.com
Keywords：Cervical tumors Papilloma virus Gene p53
参考文献：
[1]Howley pM. Role of the human papilomavirus in human cancer. Cancer Res,1991,51:5019-5022
[2]Stonbridge Euman. Tumor suppressor genes. Annul Rev Genet, 1990,24:615
[3]Harris CC. p53: At the Crossroads of Molecullar carcinogenesis and Risk Assessment. Science, 1993,262:1980-1981
, http://www.100md.com
[4]Munger K, Phelps WC, Bubb V. The E6 and E7 gene of human papillovirus type 16 together are necessary sufficient for transformation ofprimary human keratinocytes.Jvirol.1989,63:4417-21
[5]MirandaT，Paola M，John J，et al.HPV-18E6 mediated inhitition of p53 DNA binding activity is independent of E6 induced degradation.Oncogene.1995,10:261-268
[6]Mark S，Lechner,Laimonis A，Laimins.Inhibition of p53 DNA binding by human papillovirus E6 protein.Journal of virology.1994,68:4262-4273
[7]Smith ML,Bortnick RA,Sheikh MS,et al.Chromatin relaxation by overex pression of mutant p53，HPVl6-E6，or cyclin G transgenes.ExpCell Res.1998,242:235-243
收稿日期：1999-10-09, http://www.100md.com