颗粒膜蛋白的表达与狼疮肾炎的关系研究
作者：董光富 叶任高 李幼姬 汪涛 余学清
单位：510080广州，中山医科大学附属第一医院肾内科卫生部肾脏病临床研究重点实验室
关键词：狼疮肾炎；免疫组织化学；颗粒膜蛋白
中华肾脏病杂志000308摘 要：目的了解血浆黏附分子颗粒膜蛋白(GMP-140)水平及其在肾组织的表达与狼疮肾炎(LN)活动、肾脏病理及肾功能损害的关系。方法用酶联免疫吸附法(ELISA)测定血浆GMP-140水平，并用免疫组织化学染色法，观察正常肾和狼疮肾组织GMP-140表达。结果LN患者较正常人以及活动LN较非活动LN，血GMP-140水平显著升高；LN肾组织GMP-140表达较正常肾组织明显增强，且狼疮肾组织GMP-140+细胞数与血浆GMP-140水平和狼疮肾活动指数明显正相关，与肾脏病理和功能损害有一定相关关系。结论GMP-140在介导LN中起重要作用，其血浆水平或肾组织表达水平可作为反映LN活动和进行性肾损害的指标。
, http://www.100md.com
Relationship between the expression of GMP-140 and lupus nephritis
DONG Guangfu,YE Rengao,LI Youji
(Department of Nephology, Key Clinical Kidney Research Institute of Ministry of Health, First Affiliated Hospital , Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China)
Abstract：Objective To investigate the plasma level and in situ expression of granular membrane protein(GMP-140) in lupus nephritis (LN), and their correlations with the renal injuries. Methods Plasma GMP-140 was measured with ELISA in LN patients and normal control. GMP-140 expression in the kidney was measured by a microwave-based immunohistochemistry. Results There was a significant elevation of plasma GMP-140 level and marked up-regulation of GMP-140 expression in renal tissue of LN patients as compared to the normal control. The elevated level of plasma GMP-140 and its increased expression in were strongly correlated with LN activity, renal function and histological changes. Conclusion GMP-140 may play an important role in the mediation of LN pathogenesis and its plasma level and expression in renal tissue may be used as an index of the disease activity and progressive renal injuries.
, 百拇医药
Keywords：Lupus nephritis; Immunohistochemistry; Granular membrane protein
基金项目：广东省中医药管理局基金资助项目(98375)
参考文献：
[1]谢存，叶任高，张晓林，等.一种狼疮性肾炎的临床活动性指数.中华肾脏病杂志，1997，13：206-208.
[2]William GC. Pathogenesis of glomerular damage in glomerulonephritis. Nephrol Dial Transplant, 1998, 13 Suppl 1: 10-18.
[3]Canton AD. Adhesion molecules in renal disease. Kidney Int 1995, 48: 1687-1692.
, http://www.100md.com
[4]Mcever RP. GMP-140: a receptor for neutrophils and monocytes on aetivated platelets and endothelium. J Cell Biochem, 1991, 45: 156-161.
[5]Oda T, Kimura M. Hishida A. et al. Cell-to-cell interaetion is required to induce proteinuria in situ inmmne complex glomerulonephritis. J Lab Clin Med, 1998, 132: 112-123.
[6]Jeffrey LB. Platelets in glomerular desease. Nephron, 1997, 77: 378-393.
[7]Giuseppe D'A. Tubulointerstitial damage in glomerular diseases: its role in the progression of the renal damage. Nephrol Dial Transplant, 1998, 13 Suppl 1: 80-85.
[8]曾丽霞.叶任高.李幼姬，等.狼疮性肾炎小管间质病理改变的临床意义.中华内科杂志，1998，37：536-539.
收稿日期：1999-05-11, 百拇医药
单位：510080广州，中山医科大学附属第一医院肾内科卫生部肾脏病临床研究重点实验室
关键词：狼疮肾炎；免疫组织化学；颗粒膜蛋白
中华肾脏病杂志000308摘 要：目的了解血浆黏附分子颗粒膜蛋白(GMP-140)水平及其在肾组织的表达与狼疮肾炎(LN)活动、肾脏病理及肾功能损害的关系。方法用酶联免疫吸附法(ELISA)测定血浆GMP-140水平，并用免疫组织化学染色法，观察正常肾和狼疮肾组织GMP-140表达。结果LN患者较正常人以及活动LN较非活动LN，血GMP-140水平显著升高；LN肾组织GMP-140表达较正常肾组织明显增强，且狼疮肾组织GMP-140+细胞数与血浆GMP-140水平和狼疮肾活动指数明显正相关，与肾脏病理和功能损害有一定相关关系。结论GMP-140在介导LN中起重要作用，其血浆水平或肾组织表达水平可作为反映LN活动和进行性肾损害的指标。
, http://www.100md.com
Relationship between the expression of GMP-140 and lupus nephritis
DONG Guangfu,YE Rengao,LI Youji
(Department of Nephology, Key Clinical Kidney Research Institute of Ministry of Health, First Affiliated Hospital , Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China)
Abstract：Objective To investigate the plasma level and in situ expression of granular membrane protein(GMP-140) in lupus nephritis (LN), and their correlations with the renal injuries. Methods Plasma GMP-140 was measured with ELISA in LN patients and normal control. GMP-140 expression in the kidney was measured by a microwave-based immunohistochemistry. Results There was a significant elevation of plasma GMP-140 level and marked up-regulation of GMP-140 expression in renal tissue of LN patients as compared to the normal control. The elevated level of plasma GMP-140 and its increased expression in were strongly correlated with LN activity, renal function and histological changes. Conclusion GMP-140 may play an important role in the mediation of LN pathogenesis and its plasma level and expression in renal tissue may be used as an index of the disease activity and progressive renal injuries.
, 百拇医药
Keywords：Lupus nephritis; Immunohistochemistry; Granular membrane protein
基金项目：广东省中医药管理局基金资助项目(98375)
参考文献：
[1]谢存，叶任高，张晓林，等.一种狼疮性肾炎的临床活动性指数.中华肾脏病杂志，1997，13：206-208.
[2]William GC. Pathogenesis of glomerular damage in glomerulonephritis. Nephrol Dial Transplant, 1998, 13 Suppl 1: 10-18.
[3]Canton AD. Adhesion molecules in renal disease. Kidney Int 1995, 48: 1687-1692.
, http://www.100md.com
[4]Mcever RP. GMP-140: a receptor for neutrophils and monocytes on aetivated platelets and endothelium. J Cell Biochem, 1991, 45: 156-161.
[5]Oda T, Kimura M. Hishida A. et al. Cell-to-cell interaetion is required to induce proteinuria in situ inmmne complex glomerulonephritis. J Lab Clin Med, 1998, 132: 112-123.
[6]Jeffrey LB. Platelets in glomerular desease. Nephron, 1997, 77: 378-393.
[7]Giuseppe D'A. Tubulointerstitial damage in glomerular diseases: its role in the progression of the renal damage. Nephrol Dial Transplant, 1998, 13 Suppl 1: 80-85.
[8]曾丽霞.叶任高.李幼姬，等.狼疮性肾炎小管间质病理改变的临床意义.中华内科杂志，1998，37：536-539.
收稿日期：1999-05-11, 百拇医药