人类关节滑膜细胞表达晚期糖基化终产物受体
作者：侯凡凡 蒋建平 张训
单位：510515广州，第一军医大学南方医院肾内科 中国人民解放军肾病中心
关键词：关节滑膜细胞；晚期糖基化终产物；受体
中华肾脏病杂志000307摘 要：目的进一步探讨晚期糖基化终产物修饰的β2微球蛋白(AGE-β2m)对关节固有细胞的生物学作用，确定人类关节滑膜细胞是否表达对AGE特异的受体。方法分离、培养人关节A型和B型滑膜细胞，用免疫组织化学法及流式细胞仪法分别观察滑膜细胞表面AGE受体1(AGE-R1)、AGE受体2(AGE-R2)、AGE受体3(ACE-R3)及AGE受体(RAGE)的表达，用逆转录-聚合酶链反应(RT-PCR)技术检测滑膜细胞内各种AGE受体的mRNA水平。结果A、B两型细胞均表达RAGE和AGE-R3，两种受体均分布于细胞表面，RT-PCR示滑膜A、B两型细胞内存在RAGE和AGE-R3mRNA。两型滑膜细胞均不表达AGE-R1和AGE-R2。结论人类关节滑膜A、B两型细胞表达RAGE和AGE-R3，提示这类关节固有细胞参与了AGE的代谢，透析相关性淀粉样变时也可能成为AGE病理生物效应的靶细胞。
, 百拇医药
Expression of advanced glycation end products receptors on human joint synovial cells
HOU Fanfan,JIANG Jianping,ZHANG Xun
(Division of Nephrology , Nanfang Hospital , Guangzhou 510515, China)
Abstract：Objective To determine the expression of advanced glycation end products(AGE) binding proteins on human joint synovial cells for elucidating the pathobiological effects of β2m modified with AGE(AGE-β2m) on joint resident cells. Methods Type A and type B synovial cells were isolated and cultured in vitro. The expression of AGE receptor 1 (AGE-R1), AGE receptor 2 (AGE-R2), AGE receptor 3 (AGE-R3) and 35 KD receptor for AGE(RAGE) on synoviocytes were detected by immunofluorescent staining using specific antibodies and flow cytometric analyses, mRNA of AGE receptors was examined by RT-PCR techniques. Results RAGE and AGE-R3, but not AGE-R1 and AGE-R2, were constitutively expressed on the membrane surface of both type A and type B synovial cells. These two types of synovial cell also expressed mRNA of RAGE and AGE-R3. Conclusion Human joint synovial cells express specific AGE binding proteins, RAGE and AGE-R3, suggesting that these cells may be involved in AGE metabolism and might be the target of the biological effects of AGEs in dialysis-related amyloidosis.
, 百拇医药
Keywords：Synovial cells; Advanced glycation end products; Receptor
基金项目：国家自然科学基金资助项目(39970341)
参考文献：
[1]侯凡凡，张训.透析相关性淀粉样变诊断与治疗的进展.中华内科杂志，1997，36：135-137.
[2]Miyata T, Oda O, Inagi R, et al. β2-microglobulin modified with advanced glycation end products is a major component of hemodialysis-associated amyloidosis. J Clin Invest, 1993, 29: 1243-1247.
, 百拇医药
[3]Miyata T, Inagi R, Iida Y, et al. Involvement of β2-microglobulin modified with advanced glycation end products in the pathogenesis of haemodialysis-associated amyloidosis : induction of human monocyte chemotaxis and macrophage secretion of tumor necrosis factor-α and interleukin 1. J Clin Invest, 1994, 93: 521-528.
[4]Garbar C, Jadoul M, Noel H, et al. Histological characteristics of sternoclavicular β2-microglobulin amyloidosis and clues for its histogenesis. Kidney Int, 1999, 55: 1983-1990.
, 百拇医药
[5]Miyata T, Hori O, Zhang JH, et al. The receptor for advanced glycation end products(RAGE) is a central mediator of the interaction of AGE-β2 microglobulin with human mononuclear phagocytes via an oxidant-sensitive pathway. J Clin Invest, 1996, 98: 1088-1094.
[6]Chomcznski P, Jacchi N. Single-step method of RNA isolation by acid guanidium-thicyanae-phenol-chloroform extraction. Anal Biochem, 1987, 162: 156-159.
[7]Li J J, Dickson D, Hof PR, et al. Receptors for advanced glycosylation end products in human brain: role in brain homeostasis. Mol Med, 1998, 4: 46-60.
, 百拇医药
[8]Neeper M, Schmidt AM, Brett J, et al. Cloning and expression of a cell surface receptor for advanced glycosylation end products of proteins. J Biol Chem, 1992, 267: 14998-15004.
[9]罗政良，张端莲.软骨、骨与关节.见：成金忠，主编.组织学.第2版.北京：人民卫生出版社，1993.334-336.
[10]Vlassara H. Protein glycation in the kidney; role in diabetes and aging. Kidney Int, 1996, 1795-1799.
[11]黄宇峰，林善锬，程梅芬.聚合酶链反应检测糖化终末产物受体基因并调查其在大鼠务器官的分布.中华肾脏病杂志，1996，12：11-14.
, 百拇医药
[12]Owen WF Jr, Hou FF. Stuart RO, et al. β2-microglobulin modified with advanced glycation end products modulates collagen synthesis by human fibroblasts. Kidney Int, 1998, 53: 1365-1373.
[13]侯凡凡，张训.糖基化终产物修饰的β2微球蛋白在透析相关性淀粉样变发病机制中的作用.中华内科杂志，1998，37：560-562.
[14]Schmidt AM, Hori O, Brett J, et al. Cellular receptors for advanced glycation end products. Implications for induction of oxidant stress and cellular dysfunction in the pathogenesis of vascular lesions. Arterioscler Thromb, 1994, 14: 1521-152.
收稿日期：2000-01-08, 百拇医药
单位：510515广州，第一军医大学南方医院肾内科 中国人民解放军肾病中心
关键词：关节滑膜细胞；晚期糖基化终产物；受体
中华肾脏病杂志000307摘 要：目的进一步探讨晚期糖基化终产物修饰的β2微球蛋白(AGE-β2m)对关节固有细胞的生物学作用，确定人类关节滑膜细胞是否表达对AGE特异的受体。方法分离、培养人关节A型和B型滑膜细胞，用免疫组织化学法及流式细胞仪法分别观察滑膜细胞表面AGE受体1(AGE-R1)、AGE受体2(AGE-R2)、AGE受体3(ACE-R3)及AGE受体(RAGE)的表达，用逆转录-聚合酶链反应(RT-PCR)技术检测滑膜细胞内各种AGE受体的mRNA水平。结果A、B两型细胞均表达RAGE和AGE-R3，两种受体均分布于细胞表面，RT-PCR示滑膜A、B两型细胞内存在RAGE和AGE-R3mRNA。两型滑膜细胞均不表达AGE-R1和AGE-R2。结论人类关节滑膜A、B两型细胞表达RAGE和AGE-R3，提示这类关节固有细胞参与了AGE的代谢，透析相关性淀粉样变时也可能成为AGE病理生物效应的靶细胞。
, 百拇医药
Expression of advanced glycation end products receptors on human joint synovial cells
HOU Fanfan,JIANG Jianping,ZHANG Xun
(Division of Nephrology , Nanfang Hospital , Guangzhou 510515, China)
Abstract：Objective To determine the expression of advanced glycation end products(AGE) binding proteins on human joint synovial cells for elucidating the pathobiological effects of β2m modified with AGE(AGE-β2m) on joint resident cells. Methods Type A and type B synovial cells were isolated and cultured in vitro. The expression of AGE receptor 1 (AGE-R1), AGE receptor 2 (AGE-R2), AGE receptor 3 (AGE-R3) and 35 KD receptor for AGE(RAGE) on synoviocytes were detected by immunofluorescent staining using specific antibodies and flow cytometric analyses, mRNA of AGE receptors was examined by RT-PCR techniques. Results RAGE and AGE-R3, but not AGE-R1 and AGE-R2, were constitutively expressed on the membrane surface of both type A and type B synovial cells. These two types of synovial cell also expressed mRNA of RAGE and AGE-R3. Conclusion Human joint synovial cells express specific AGE binding proteins, RAGE and AGE-R3, suggesting that these cells may be involved in AGE metabolism and might be the target of the biological effects of AGEs in dialysis-related amyloidosis.
, 百拇医药
Keywords：Synovial cells; Advanced glycation end products; Receptor
基金项目：国家自然科学基金资助项目(39970341)
参考文献：
[1]侯凡凡，张训.透析相关性淀粉样变诊断与治疗的进展.中华内科杂志，1997，36：135-137.
[2]Miyata T, Oda O, Inagi R, et al. β2-microglobulin modified with advanced glycation end products is a major component of hemodialysis-associated amyloidosis. J Clin Invest, 1993, 29: 1243-1247.
, 百拇医药
[3]Miyata T, Inagi R, Iida Y, et al. Involvement of β2-microglobulin modified with advanced glycation end products in the pathogenesis of haemodialysis-associated amyloidosis : induction of human monocyte chemotaxis and macrophage secretion of tumor necrosis factor-α and interleukin 1. J Clin Invest, 1994, 93: 521-528.
[4]Garbar C, Jadoul M, Noel H, et al. Histological characteristics of sternoclavicular β2-microglobulin amyloidosis and clues for its histogenesis. Kidney Int, 1999, 55: 1983-1990.
, 百拇医药
[5]Miyata T, Hori O, Zhang JH, et al. The receptor for advanced glycation end products(RAGE) is a central mediator of the interaction of AGE-β2 microglobulin with human mononuclear phagocytes via an oxidant-sensitive pathway. J Clin Invest, 1996, 98: 1088-1094.
[6]Chomcznski P, Jacchi N. Single-step method of RNA isolation by acid guanidium-thicyanae-phenol-chloroform extraction. Anal Biochem, 1987, 162: 156-159.
[7]Li J J, Dickson D, Hof PR, et al. Receptors for advanced glycosylation end products in human brain: role in brain homeostasis. Mol Med, 1998, 4: 46-60.
, 百拇医药
[8]Neeper M, Schmidt AM, Brett J, et al. Cloning and expression of a cell surface receptor for advanced glycosylation end products of proteins. J Biol Chem, 1992, 267: 14998-15004.
[9]罗政良，张端莲.软骨、骨与关节.见：成金忠，主编.组织学.第2版.北京：人民卫生出版社，1993.334-336.
[10]Vlassara H. Protein glycation in the kidney; role in diabetes and aging. Kidney Int, 1996, 1795-1799.
[11]黄宇峰，林善锬，程梅芬.聚合酶链反应检测糖化终末产物受体基因并调查其在大鼠务器官的分布.中华肾脏病杂志，1996，12：11-14.
, 百拇医药
[12]Owen WF Jr, Hou FF. Stuart RO, et al. β2-microglobulin modified with advanced glycation end products modulates collagen synthesis by human fibroblasts. Kidney Int, 1998, 53: 1365-1373.
[13]侯凡凡，张训.糖基化终产物修饰的β2微球蛋白在透析相关性淀粉样变发病机制中的作用.中华内科杂志，1998，37：560-562.
[14]Schmidt AM, Hori O, Brett J, et al. Cellular receptors for advanced glycation end products. Implications for induction of oxidant stress and cellular dysfunction in the pathogenesis of vascular lesions. Arterioscler Thromb, 1994, 14: 1521-152.
收稿日期：2000-01-08, 百拇医药