哺乳动物心脏的缝管连接
作者：邵素霞 张雷
单位：石家庄河北师范大学实验中心 050016， 河北医科大学组胚教研室 河北 050017
关键词：
解剖科学进展000414摘要 缝管连接(gap junction)是相邻细胞质膜的特 化结构，允许相邻细胞间的离子和小分子代谢物质直接进行交换。在心肌组织中缝管连接具有重要的生理功能。本文综述了心肌组织中缝管连接的分布、超微结构、连接蛋白的种类和分子结构及其在发育中的变化，并对其研究方法进行了较详细的阐述。
参考文献
1，Warner Anne. The gap junction. Journal of Cell Science, 1988, 89: 1
2，成令忠主编.组织学，第二版.北京：人民卫生出版社，1993,188～190
, 百拇医药
3，Forbes MS, Sperelakis N. Intercalated discs of mammalian heart: A review of structure and founction. Tissue Cell, 1985, 17(5): 605
4，Mcrnutt NS, Weinstein RS. The ultrastructure of the nexus. A correlaed thin section and freeze-cleave study. J cell Biol, 1970, 47: 666
5，Mcuntt NS, Weinstein RS. Membrane ultrastructure at ammalian intercellular junctions. In Progress in Biophysics and Molecular Biology, 1973, 26: 45
, 百拇医药
6，Zampighi G. On gap junction structure. J Cell Biol, 1986, 86: 190
7，Zhang Lei, Keisuke Ina, Hirokazu Kitamuram, et al. The intercalated disc of monkey myocardial cell and purkinje fibers as revealed by scanning electromicroscepy. Arch Histol Cytol, 1996, 59(5): 453
8，Severs NJ, Gourdie RG, Harfst E, et al. Intercellular junctions and the application of microscopical techniques: the cardiac gap junction as a case model. J Microsc, 1993, 169(3): 299
, 百拇医药
9，Spach MS, Dolber PC. Relating extracellular potential and their derivatives to anistropic propagation at a microscopic level in human cadiac muscle. Cir Res, 1986, 58: 356
10，Robert H, Cotorn H, Saffitz E. Distribution and threedimensional sturcture of intercellutar junctions in canine myocardium. Cir. Res, 1989, 64: 563
11，邵素霞，张雷，鹿萍.胎儿心肌细胞的超微结构研究.中国电子显微学报，1998, 17(4)： 417
12，Petra WC, Szabolcs V, Maygen EM, et al. Immunohistochemical delineation of the conduction system. Cir. Res, 1993, 73: 473
, 百拇医药
13，Beyer EC, Paul DL, Goodenough DA. Connexin family of gap junction prolein. J Membr Biol, 1990, 116: 187
14，Laird HW, Revel JP. Biochemical and immunochemical analysis of the arrangement of connexin 43 in rat heart gap junction membranes. J Cell Science, 1990, 97: 109
15，Maria JA, Van Kempen, Antoon FM, Mooman, WH, et al. Spatial distribution of connexin 43 the cardiac gap junction protein in the developing and adult rat heart. Circ Res, 1991, 68: 163
, 百拇医药
16，Beyer EC, Kistler J, Paul DL, et al. Antisera directed aginst connexin 43 peptides ratct with a 43-KD protein localized to gap junctions in myocardium and other tissue. J Cell Biol, 1989, 108: 595
17，Kadle R, Zhang JT. Nicholson BJ, et al. Tissue-specific distribution of differentially phosphorylated forms of Cx43. Mol Cell Biol, 1991, 11: 363
18，Beyer E, Paul Goodenough DA. Connexin 43, A protein from rat heart homologous to gap junction protein from liver. J Cell Biol, 1987, 195: 2621
, 百拇医药
19，Beyer EC, Steinberg TH. Evidence that the gap junction protein connexin 43 is ATP-induced pore of mouse macrophage. J Biol Chem, 1991, 266: 7971
20，Fishman GI, Spray DC, Leinwand LA. Molecular charactenzation and founctional expression of the human cardiac gap junction channel. J Cell Biol, 1999, 111: 589
21，Lash JA, Crister ES, Pressler ML. Cloning of a gap junctional protein frim vascular smooth muscle and expression in two-all mouse embryos. J Biol Chem, 1990, 265: 13113
, 百拇医药
22，Daniel G, Jarry Giuchard, Velde T, et al. Restricted distribution of connexin 40, a gap junctional protein, in mammalian heart. Circres, 1994, 74: 839
23，Willecke HKR, Dahle E. Mouse connexin 37:cloning and founctional expression of a gap junction gene highly expressed in lung. J Cell Biol, 1991, 114: 1049
24，Kanter HI, Saffitz E, Beyer EC. Cardiac myocytes express multiple gap junction protein. Cir Res, 1992, 70: 438
, 百拇医药
25，Gourdie RG, Harfst E, Severs NJ, et al. Cardiac gap junctions in Rat ventricle: lacalization using site-directed antibodies and Laser scanning confocal microcopy. Cardioscience, 1990, 1:75
26，Manjunath CK, Nicholson BJD, Teplow, et al. The cardiac gap junction protein (Mr 4700) has a tissue-specific cytoplasmic domain of Mr 17000 at its carboxyl teminus. Biolchem Biophys Res Commun, 1978, 142: 228
27，Yancey SB, John SA, Lal R, et al. The 43-KD polypeptide of heart gap junctions. Immun olocalization, topology and functional domains. J Cell Biol, 1989, 108: 2241
, 百拇医药
28，Aoumari A, Fromaget C, Dupont E, et al. Conservation of cytoplasmic carboxy-terminal domain of connexin 43, a gapjunctional protei nin mammal heart andbrain. J Membr Biol, 1990, 115: 229
29，Gros D, Challioe CE. Early development of gapjunctions between the mouse embryonic myocardial cells: A freeze-etching study. Experimentia, 1976, 32: 996
30，Yosaburo S. Ultrastructure changes during development of gap junctionsin rabbit left ventricular myocardial cells. J Ultrastructure Res, 1980,71: 258
, 百拇医药
31，Van Kempen MJA, Vermeulen JLM, Moorman AF, et al. Developmental changes of connexin40 and connexin43 mRNA distribution patterns in the rat heart. Cardiovasc Res, 1996, 32(5):886
32，Angst BD, Laceq URK, Severs K, et al. Dissociated spatial patterning of gap junctions and cell adnesion junctions during postnatal differentiation of entricular myocardium. Cir Res, 1997,80(1):88
33，Fishman IG, Elliot L, Hertzberg DC, et al. Expression of connexin43 in the developing rat heart. Cir Res, 1991, 68:782
, http://www.100md.com
34，Sternberger LA. The unlabelled antibody enzyme method of immunohistochemistry. J Histochem Cytochem, 1970, 18:315
35，蔡文琴，王泊纭，主编.实用免疫细胞化学与核酸分子杂交技术. 成都，四川科学出版社出版，1994，121
36，Faulk WP, Taylor GM. An immunocolloid method for the electron microscope. Immunocytochem, 1971,8:1081
37，倪灿荣主编.免疫组织化学实验新技术及应用. 北京：科学技术出版社，1993，252
38，Abdelhakim EL, Aoumari ED, Catherine F, et al. Immunolocalization of an extracellular domain of connexin 43 in rat heat gap junction. Eur J cell Biology, 1991,56: 391, http://www.100md.com
单位：石家庄河北师范大学实验中心 050016， 河北医科大学组胚教研室 河北 050017
关键词：
解剖科学进展000414摘要 缝管连接(gap junction)是相邻细胞质膜的特 化结构，允许相邻细胞间的离子和小分子代谢物质直接进行交换。在心肌组织中缝管连接具有重要的生理功能。本文综述了心肌组织中缝管连接的分布、超微结构、连接蛋白的种类和分子结构及其在发育中的变化，并对其研究方法进行了较详细的阐述。
参考文献
1，Warner Anne. The gap junction. Journal of Cell Science, 1988, 89: 1
2，成令忠主编.组织学，第二版.北京：人民卫生出版社，1993,188～190
, 百拇医药
3，Forbes MS, Sperelakis N. Intercalated discs of mammalian heart: A review of structure and founction. Tissue Cell, 1985, 17(5): 605
4，Mcrnutt NS, Weinstein RS. The ultrastructure of the nexus. A correlaed thin section and freeze-cleave study. J cell Biol, 1970, 47: 666
5，Mcuntt NS, Weinstein RS. Membrane ultrastructure at ammalian intercellular junctions. In Progress in Biophysics and Molecular Biology, 1973, 26: 45
, 百拇医药
6，Zampighi G. On gap junction structure. J Cell Biol, 1986, 86: 190
7，Zhang Lei, Keisuke Ina, Hirokazu Kitamuram, et al. The intercalated disc of monkey myocardial cell and purkinje fibers as revealed by scanning electromicroscepy. Arch Histol Cytol, 1996, 59(5): 453
8，Severs NJ, Gourdie RG, Harfst E, et al. Intercellular junctions and the application of microscopical techniques: the cardiac gap junction as a case model. J Microsc, 1993, 169(3): 299
, 百拇医药
9，Spach MS, Dolber PC. Relating extracellular potential and their derivatives to anistropic propagation at a microscopic level in human cadiac muscle. Cir Res, 1986, 58: 356
10，Robert H, Cotorn H, Saffitz E. Distribution and threedimensional sturcture of intercellutar junctions in canine myocardium. Cir. Res, 1989, 64: 563
11，邵素霞，张雷，鹿萍.胎儿心肌细胞的超微结构研究.中国电子显微学报，1998, 17(4)： 417
12，Petra WC, Szabolcs V, Maygen EM, et al. Immunohistochemical delineation of the conduction system. Cir. Res, 1993, 73: 473
, 百拇医药
13，Beyer EC, Paul DL, Goodenough DA. Connexin family of gap junction prolein. J Membr Biol, 1990, 116: 187
14，Laird HW, Revel JP. Biochemical and immunochemical analysis of the arrangement of connexin 43 in rat heart gap junction membranes. J Cell Science, 1990, 97: 109
15，Maria JA, Van Kempen, Antoon FM, Mooman, WH, et al. Spatial distribution of connexin 43 the cardiac gap junction protein in the developing and adult rat heart. Circ Res, 1991, 68: 163
, 百拇医药
16，Beyer EC, Kistler J, Paul DL, et al. Antisera directed aginst connexin 43 peptides ratct with a 43-KD protein localized to gap junctions in myocardium and other tissue. J Cell Biol, 1989, 108: 595
17，Kadle R, Zhang JT. Nicholson BJ, et al. Tissue-specific distribution of differentially phosphorylated forms of Cx43. Mol Cell Biol, 1991, 11: 363
18，Beyer E, Paul Goodenough DA. Connexin 43, A protein from rat heart homologous to gap junction protein from liver. J Cell Biol, 1987, 195: 2621
, 百拇医药
19，Beyer EC, Steinberg TH. Evidence that the gap junction protein connexin 43 is ATP-induced pore of mouse macrophage. J Biol Chem, 1991, 266: 7971
20，Fishman GI, Spray DC, Leinwand LA. Molecular charactenzation and founctional expression of the human cardiac gap junction channel. J Cell Biol, 1999, 111: 589
21，Lash JA, Crister ES, Pressler ML. Cloning of a gap junctional protein frim vascular smooth muscle and expression in two-all mouse embryos. J Biol Chem, 1990, 265: 13113
, 百拇医药
22，Daniel G, Jarry Giuchard, Velde T, et al. Restricted distribution of connexin 40, a gap junctional protein, in mammalian heart. Circres, 1994, 74: 839
23，Willecke HKR, Dahle E. Mouse connexin 37:cloning and founctional expression of a gap junction gene highly expressed in lung. J Cell Biol, 1991, 114: 1049
24，Kanter HI, Saffitz E, Beyer EC. Cardiac myocytes express multiple gap junction protein. Cir Res, 1992, 70: 438
, 百拇医药
25，Gourdie RG, Harfst E, Severs NJ, et al. Cardiac gap junctions in Rat ventricle: lacalization using site-directed antibodies and Laser scanning confocal microcopy. Cardioscience, 1990, 1:75
26，Manjunath CK, Nicholson BJD, Teplow, et al. The cardiac gap junction protein (Mr 4700) has a tissue-specific cytoplasmic domain of Mr 17000 at its carboxyl teminus. Biolchem Biophys Res Commun, 1978, 142: 228
27，Yancey SB, John SA, Lal R, et al. The 43-KD polypeptide of heart gap junctions. Immun olocalization, topology and functional domains. J Cell Biol, 1989, 108: 2241
, 百拇医药
28，Aoumari A, Fromaget C, Dupont E, et al. Conservation of cytoplasmic carboxy-terminal domain of connexin 43, a gapjunctional protei nin mammal heart andbrain. J Membr Biol, 1990, 115: 229
29，Gros D, Challioe CE. Early development of gapjunctions between the mouse embryonic myocardial cells: A freeze-etching study. Experimentia, 1976, 32: 996
30，Yosaburo S. Ultrastructure changes during development of gap junctionsin rabbit left ventricular myocardial cells. J Ultrastructure Res, 1980,71: 258
, 百拇医药
31，Van Kempen MJA, Vermeulen JLM, Moorman AF, et al. Developmental changes of connexin40 and connexin43 mRNA distribution patterns in the rat heart. Cardiovasc Res, 1996, 32(5):886
32，Angst BD, Laceq URK, Severs K, et al. Dissociated spatial patterning of gap junctions and cell adnesion junctions during postnatal differentiation of entricular myocardium. Cir Res, 1997,80(1):88
33，Fishman IG, Elliot L, Hertzberg DC, et al. Expression of connexin43 in the developing rat heart. Cir Res, 1991, 68:782
, http://www.100md.com
34，Sternberger LA. The unlabelled antibody enzyme method of immunohistochemistry. J Histochem Cytochem, 1970, 18:315
35，蔡文琴，王泊纭，主编.实用免疫细胞化学与核酸分子杂交技术. 成都，四川科学出版社出版，1994，121
36，Faulk WP, Taylor GM. An immunocolloid method for the electron microscope. Immunocytochem, 1971,8:1081
37，倪灿荣主编.免疫组织化学实验新技术及应用. 北京：科学技术出版社，1993，252
38，Abdelhakim EL, Aoumari ED, Catherine F, et al. Immunolocalization of an extracellular domain of connexin 43 in rat heat gap junction. Eur J cell Biology, 1991,56: 391, http://www.100md.com