大鼠心肌缺血/再灌注损伤模型的改进与评价
作者：王淑侠 裴著果 兰小莉 王吉文
单位：王淑侠 裴著果(中国医科大学第二临床学院核医学科 沈阳 110003)；兰小莉(中国人民解放军第202医院心内科)；王吉文(中山医科大学附属第二医院急诊科)
关键词：心肌再灌注损伤；大鼠；模型，TTC染色法
解剖科学进展000425 摘要 大鼠心肌缺血/再灌注损伤是模拟人类心梗及溶栓再通治疗，研究缺血预适应机制，评价抗心律失常药物疗效常用的动物模型。本文在传统造模方法的基础上进行了一定的改进，采用在压管下加一小垫片的方法，减少了传统推管法对心表面的机械损伤，方法简便，冠脉阻断确实。结果：大鼠左冠状动脉主干缺血30′，再灌120′，危险区/左室重量比为55%±5%，坏死区/危险区为58%±6%(N=10)。心肌酶谱结果：CK4301±251u/l,CK-MB 2288±328u/l, LDH 2329±216u/l, AST 371±57u/l(N=5)。
Improvement on Techniques for Making Myocardial Reperfusion Injury Rat Model
Wang Shuxia, Lan Xiao Li, Wang Jiwen, Pei Zhuguo
(Department of nuclear medicine, No2 affiliated hospital, China Medical University , 110003)
Abstract Rat myocardial ischemia/reperfusion in situ is the most frequently used animal model for studing coronary artery disease, ischemic preconditioning and selecting antiarrhythmic drugs. In this paper, we reported our improvement on making such model. After 30 minutes occlusion and 120 minutes reperfusion of left coronary artery, area at risk/left ventricle was 55%±5%, Infarct area/area at risk was 58%±6%(n=10). Myocardial enzyme spectrum results: CK 4301±251u/l,CK-MB 2288±328u/l, LDH 2329±216u/l, AST 371±57u/l(n=5).
Key word Myocardial reperfusion injury; rat; models; TTC method
参考文献
1，Selye H. Simple techniques for surgical occlusion of coronary vessels in the rat. Angiology, 1960,11:398
2，Pfefer MA. Myocardial infarct and ventricular function in rats. Circ Res, 1979,44:503
3，Fisbein MC. Experimental Myocardial infarction in the rat. Am J Pathol, 1978, 90:57
4，Wexler Bc. Repeated myocardial infarction in non-arteriosclerotic and arteriosclerotic prague-Dawley rats. Br J Exp pathol, 1978,59(6):564
5，汪谦.现代医学实验方法.北京，人民卫生出版社，1997，905, 百拇医药
单位：王淑侠 裴著果(中国医科大学第二临床学院核医学科 沈阳 110003)；兰小莉(中国人民解放军第202医院心内科)；王吉文(中山医科大学附属第二医院急诊科)
关键词：心肌再灌注损伤；大鼠；模型，TTC染色法
解剖科学进展000425 摘要 大鼠心肌缺血/再灌注损伤是模拟人类心梗及溶栓再通治疗，研究缺血预适应机制，评价抗心律失常药物疗效常用的动物模型。本文在传统造模方法的基础上进行了一定的改进，采用在压管下加一小垫片的方法，减少了传统推管法对心表面的机械损伤，方法简便，冠脉阻断确实。结果：大鼠左冠状动脉主干缺血30′，再灌120′，危险区/左室重量比为55%±5%，坏死区/危险区为58%±6%(N=10)。心肌酶谱结果：CK4301±251u/l,CK-MB 2288±328u/l, LDH 2329±216u/l, AST 371±57u/l(N=5)。
Improvement on Techniques for Making Myocardial Reperfusion Injury Rat Model
Wang Shuxia, Lan Xiao Li, Wang Jiwen, Pei Zhuguo
(Department of nuclear medicine, No2 affiliated hospital, China Medical University , 110003)
Abstract Rat myocardial ischemia/reperfusion in situ is the most frequently used animal model for studing coronary artery disease, ischemic preconditioning and selecting antiarrhythmic drugs. In this paper, we reported our improvement on making such model. After 30 minutes occlusion and 120 minutes reperfusion of left coronary artery, area at risk/left ventricle was 55%±5%, Infarct area/area at risk was 58%±6%(n=10). Myocardial enzyme spectrum results: CK 4301±251u/l,CK-MB 2288±328u/l, LDH 2329±216u/l, AST 371±57u/l(n=5).
Key word Myocardial reperfusion injury; rat; models; TTC method
参考文献
1，Selye H. Simple techniques for surgical occlusion of coronary vessels in the rat. Angiology, 1960,11:398
2，Pfefer MA. Myocardial infarct and ventricular function in rats. Circ Res, 1979,44:503
3，Fisbein MC. Experimental Myocardial infarction in the rat. Am J Pathol, 1978, 90:57
4，Wexler Bc. Repeated myocardial infarction in non-arteriosclerotic and arteriosclerotic prague-Dawley rats. Br J Exp pathol, 1978,59(6):564
5，汪谦.现代医学实验方法.北京，人民卫生出版社，1997，905, 百拇医药