内皮素在大鼠系膜增生性肾小球肾炎中的表达
作者：李美花 谌贻璞 黄泓 鄂洁
单位：100034 北京医科大学第一医院肾内科
关键词：内皮素-1；基因；表达；系膜增生性肾小球肾炎
中华肾脏病杂志000506摘 要：目的研究内皮素-1(ET-1)在系膜增生性肾小球肾炎(MsPGN)模型中的表达。方法以小鼠抗大鼠Thy1.1单克隆抗体单次尾静脉注射诱导的大鼠MsPGN模型，病理观察分析该模型的系膜细胞和系膜基质增生；Northern杂交分析肾小球前内皮素(ppET-1)mRNA表达水平；放射免疫分析(RIA)测定肾小球ET-1蛋白水平。结果在大鼠MsPGN模型中，第2d系膜细胞溶解消失，第4d系膜细胞增生，第6d系膜细胞明显增生伴系膜基质增生。MsPGN大鼠肾小球ppET-1mRNA表达上调，于第4d的表达趋势最强。肾小球ET-1蛋白分别于第2d、第6d两次明显升高[(87.13±20.16)ng/g，(100.19±14.16)ng/g]，与对照组[(46.50±4.28)ng/g]比较，差异有显著性意义(P<0.05)。结论在MsPGN模型中，伴随着系膜细胞和系膜基质增生，肾小球产生的ET-1增加，提示ET-1可能参与了体内系膜细胞和系膜基质增生。
, http://www.100md.com
Glomerular expression of endothelin-1 in anti-Thy1.1 glomerulonephritis
LI Meihua ,CHEN Yipu ,HUANG Hong,et al.
(Department of Nephrology,First Hospital,Beijing Medical University,Beijing 100034,China)
Abstract：Objective To investigate the expression of endothelin-1 (ET-1) in mesangioproliferative glomerulonephritis (MsPGN) in rats.Methods MsPGN rats were induced by a single intravenous injection of monoelonal anti-thy1.1 antibody.Glomerular lesions including mesangial cell proliferation and mesangial matrix expansion were observed pathologically.Preproendothelin-1 (ppET-1)mRNA expression in glomeruli were analyzed by Northern blot hybridization.Concentration of ET-1 in glomeruli were measured with radioimmunoassay (RIA).Results In MsPGN rats,mesangial cells dissolved at day 2,proliferated at day 4,proliferated obviously with mesangial matrix expansion at day 6.In parallel with mesangial cell proliferation and mesangial matrix expansion,glomernlar ppET-1 mRNA expression was up-regulated with its dominance at day 4.ET-1 protein content in glomemli was elevated at day 2(87.13±0.16) ng/g,day 6 (100.19±4.16) ng/g respectively,compared to control group (46.50±4.28) ng/g ,P<0.05.Conclusions In MsPGN,glomerular ET-1 production increases in association with mesangial cell proliferation and mesangial matrix expansion.ET-1 may be involved in the process of mesangial cell proliferation and mesangial matrix expansion in vivo.
, 百拇医药
Keywords：Endothelin-1;Gene;Expression;Mesangioproliferative glomerulonephritis 基金项目：国家自然科学基金资助项目(39470334)
参考文献：
[1]谌贻璞.炎症细胞与炎症介质.见：王海燕，主编.肾脏病学.第2版.北京：人民卫生出版社，1996.477-478.
[2]徐新来.细胞培养在杂交瘤细胞中的应用.见：鄂征，主编.组织培养和分子细胞学技术.第2版.北京：北京出版社，1994.207-214.
[3]金冬雁，黎孟枫，侯云德，等.分子克隆.第2版.北京：科技出版社，1992.363.
[4]Ehrenreich H.Anderson RW，Fox CH.Et al.Endothelins，peptides with potent vasoactive properties are produced by human macrophages.J Exp Med，1990，172:1741-1748.
, http://www.100md.com
[5]Bagchus WM，Hoedemaeker PhJ，Rozing J，et al.Glomerulonephritis induced by monoclonal anti-Thy1.1 antibody.Lab Invest，1986，55:680-687.
[6]Yoshimura A.Iwasaki S.Inui K.Et al.Endothelin-1 and endothelin B type receptor are induced in mesangial proliferative nephritis in the rat.Kidney Int，1995，48:1290-1297.
[7]马利军，谌贻璞，高进，等.内皮素对肾小球系膜细胞炎症效应的作用.肾脏病与透析肾移植杂志，1993，2:17-19.
[8]Chen Yipu，Sun Yijian，Lin Jingmei，et al.Research on the mechanism of endothelin inflammatory effects on human mesangial cells.Chin Med J，1997，1101 530-534.
, 百拇医药
[9]Saeada T，Haneda M，Togawa M，et al.Studies on type Ⅳ collagen production in cultured mesangial cells.Nippon Jinzo Gakkai Shi，1996，38:469-471.
[10]Iwamoto T，Tamaki K，Nakavama M，et al.Effect of endothelin 1 on fibrinolysis and plasminogen activator inhibitor 1 synthesis in rat mesangial cells.Nephron，1996，73:273.
[11]李美花，谌贻璞，张志文，等.凝血酶通过内皮素介导刺激肾小球系膜细胞增生.中华内科杂志，1999，38:153-159.
收稿日期：2000-03-21, http://www.100md.com
单位：100034 北京医科大学第一医院肾内科
关键词：内皮素-1；基因；表达；系膜增生性肾小球肾炎
中华肾脏病杂志000506摘 要：目的研究内皮素-1(ET-1)在系膜增生性肾小球肾炎(MsPGN)模型中的表达。方法以小鼠抗大鼠Thy1.1单克隆抗体单次尾静脉注射诱导的大鼠MsPGN模型，病理观察分析该模型的系膜细胞和系膜基质增生；Northern杂交分析肾小球前内皮素(ppET-1)mRNA表达水平；放射免疫分析(RIA)测定肾小球ET-1蛋白水平。结果在大鼠MsPGN模型中，第2d系膜细胞溶解消失，第4d系膜细胞增生，第6d系膜细胞明显增生伴系膜基质增生。MsPGN大鼠肾小球ppET-1mRNA表达上调，于第4d的表达趋势最强。肾小球ET-1蛋白分别于第2d、第6d两次明显升高[(87.13±20.16)ng/g，(100.19±14.16)ng/g]，与对照组[(46.50±4.28)ng/g]比较，差异有显著性意义(P<0.05)。结论在MsPGN模型中，伴随着系膜细胞和系膜基质增生，肾小球产生的ET-1增加，提示ET-1可能参与了体内系膜细胞和系膜基质增生。
, http://www.100md.com
Glomerular expression of endothelin-1 in anti-Thy1.1 glomerulonephritis
LI Meihua ,CHEN Yipu ,HUANG Hong,et al.
(Department of Nephrology,First Hospital,Beijing Medical University,Beijing 100034,China)
Abstract：Objective To investigate the expression of endothelin-1 (ET-1) in mesangioproliferative glomerulonephritis (MsPGN) in rats.Methods MsPGN rats were induced by a single intravenous injection of monoelonal anti-thy1.1 antibody.Glomerular lesions including mesangial cell proliferation and mesangial matrix expansion were observed pathologically.Preproendothelin-1 (ppET-1)mRNA expression in glomeruli were analyzed by Northern blot hybridization.Concentration of ET-1 in glomeruli were measured with radioimmunoassay (RIA).Results In MsPGN rats,mesangial cells dissolved at day 2,proliferated at day 4,proliferated obviously with mesangial matrix expansion at day 6.In parallel with mesangial cell proliferation and mesangial matrix expansion,glomernlar ppET-1 mRNA expression was up-regulated with its dominance at day 4.ET-1 protein content in glomemli was elevated at day 2(87.13±0.16) ng/g,day 6 (100.19±4.16) ng/g respectively,compared to control group (46.50±4.28) ng/g ,P<0.05.Conclusions In MsPGN,glomerular ET-1 production increases in association with mesangial cell proliferation and mesangial matrix expansion.ET-1 may be involved in the process of mesangial cell proliferation and mesangial matrix expansion in vivo.
, 百拇医药
Keywords：Endothelin-1;Gene;Expression;Mesangioproliferative glomerulonephritis 基金项目：国家自然科学基金资助项目(39470334)
参考文献：
[1]谌贻璞.炎症细胞与炎症介质.见：王海燕，主编.肾脏病学.第2版.北京：人民卫生出版社，1996.477-478.
[2]徐新来.细胞培养在杂交瘤细胞中的应用.见：鄂征，主编.组织培养和分子细胞学技术.第2版.北京：北京出版社，1994.207-214.
[3]金冬雁，黎孟枫，侯云德，等.分子克隆.第2版.北京：科技出版社，1992.363.
[4]Ehrenreich H.Anderson RW，Fox CH.Et al.Endothelins，peptides with potent vasoactive properties are produced by human macrophages.J Exp Med，1990，172:1741-1748.
, http://www.100md.com
[5]Bagchus WM，Hoedemaeker PhJ，Rozing J，et al.Glomerulonephritis induced by monoclonal anti-Thy1.1 antibody.Lab Invest，1986，55:680-687.
[6]Yoshimura A.Iwasaki S.Inui K.Et al.Endothelin-1 and endothelin B type receptor are induced in mesangial proliferative nephritis in the rat.Kidney Int，1995，48:1290-1297.
[7]马利军，谌贻璞，高进，等.内皮素对肾小球系膜细胞炎症效应的作用.肾脏病与透析肾移植杂志，1993，2:17-19.
[8]Chen Yipu，Sun Yijian，Lin Jingmei，et al.Research on the mechanism of endothelin inflammatory effects on human mesangial cells.Chin Med J，1997，1101 530-534.
, 百拇医药
[9]Saeada T，Haneda M，Togawa M，et al.Studies on type Ⅳ collagen production in cultured mesangial cells.Nippon Jinzo Gakkai Shi，1996，38:469-471.
[10]Iwamoto T，Tamaki K，Nakavama M，et al.Effect of endothelin 1 on fibrinolysis and plasminogen activator inhibitor 1 synthesis in rat mesangial cells.Nephron，1996，73:273.
[11]李美花，谌贻璞，张志文，等.凝血酶通过内皮素介导刺激肾小球系膜细胞增生.中华内科杂志，1999，38:153-159.
收稿日期：2000-03-21, http://www.100md.com